Determination of volatile components in Rosa roxburghii Tratt juice and the analysis of its contribution for aroma

  • LI Tingting ,
  • HUANG Mingzheng ,
  • TANG Weiyuan ,
  • LI Qinyang ,
  • LOU Kangning
Expand
  • 1(College of Brewing and Food Engineering, Guizhou University, Guiyang 550025, China)
    2(College of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550025, China)

Received date: 2020-10-21

  Revised date: 2020-11-13

  Online published: 2021-03-16

Abstract

In order to comprehensively analyze volatile components in Rosa roxburghii Tratt and its aroma contribution, the volatile components in Rosa roxburghii Tratt juice were qualitatively and quantitatively analyzed by head space-solid phase microextraction (HS-SPME) and solvent-assisted flavor evaporation (SAFE) combined with GC-MS. And the volatile compounds' contributions for the overall Rosa roxburghii Tratt flavors were evaluated employing odor active value (OAV). The results showed that 67 and 86 volatile compounds were detected in Rosa roxburghii tratt juice by HS-SPME and SAFE methods, and a total of 119 volatile compounds were identified which consisted of 49 esters, 28 alcohols, 9 acids, 16 aldehydes and ketones, 6 terpenes, 9 aromatics and 2 others. The two methods had different extraction efficiency for volatiles, and HS-SPME was more effective in extracting compounds with low boiling point and high volatility, while SAFE could extract more compounds with relatively high boiling point and low volatility. Moreover, comprehensive results could be obtained through combination analysis of the results from these two methods and more kinds of volatile compounds could be identified.Furthermore, OAV analysis indicated that 38 volatiles, mainly esters, alcohols, aldehydes, and ketones, contribute appreciably to the aroma of Rosa roxburghii Tratt. Among them, 16 volatile components were identified as aroma-active compounds in Rosa roxburghii Tratt juice for the first time. The study could provide a scientific basis for aroma quality control and flavor evaluation during the storage and processing of Rosa roxburghii Tratt.

Cite this article

LI Tingting , HUANG Mingzheng , TANG Weiyuan , LI Qinyang , LOU Kangning . Determination of volatile components in Rosa roxburghii Tratt juice and the analysis of its contribution for aroma[J]. Food and Fermentation Industries, 2021 , 47(4) : 237 -246 . DOI: 10.13995/j.cnki.11-1802/ts.025956

References

[1] HE J Y, ZHANG Y H, MA N, et al.Comparative analysis of multiple ingredients in Rosa roxburghii and R.sterilis fruits and their antioxidant activities[J].Journal of Functional Foods, 2016, 27:29-41.
[2] 夏仕青, 张爱华.刺梨的营养保健功能及其开发利用研究进展[J].贵州医科大学学报, 2018, 43(10):1 129-1 132;1 153.
XIA S Q, ZHANG A H.Research progress on nutrition and health care function of Rosa roxburghii Tratt and its development and utilization[J].Journal of Guizhou Medical University, 2018, 43(10):1 129-1 132;1 153.
[3] 付慧晓, 王道平, 黄丽荣, 等.刺梨和无籽刺梨挥发性香气成分分析[J].精细化工, 2012, 29(9):875-878.
FU H X, WANG D P, HUANG L R, et al.Analysis of the volatile aroma compounds of Rosa roxburghii Tratt and Rosa sterilis[J].Fine Chemicals, 2012, 29(9):875-878.
[4] 周志, 马琼, 朱玉昌, 等.GC-O-MS法分析野生刺梨汁游离态和O-糖苷键合态香气活性物质[J].食品科学, 2015, 36(22):80-84.
ZHOU Z, MA Q, ZHU Y C, et al.Analysis of free and O-glycosidically bound aroma active compounds of wild Rosa roxburghii juice by GC-O-MS[J].Food Science, 2015, 36(22):80-84.
[5] 陈思奇, 彭邦远, 丁筑红.不同贮藏温度下刺梨汁挥发性风味物质的变化特征[J].食品科技, 2019, 44(4):36-44.
CHEN S Q, PENG B Y, DING Z H.Variation characteristics of volatile flavor compounds in Rosa roxburghii juice under different storage temperature[J].Food Science and Technology, 2019, 44(4):36-44.
[6] 周志, 徐永霞, 胡昊, 等.顶空固相微萃取和同时蒸馏萃取应用于GC-MS分析野生刺梨汁挥发性成分的比较研究[J].食品科学, 2011, 32(16):279-282.
ZHOU Z, XU Y X, HU H, et al.Comparative application of head space-solid phase micro-extraction and simultaneous distillation extraction for GC-MS analysis of volatile components in Chestnut Rose (Rosa roxburghii Tratt) juice[J].Food Science, 2011, 32(16):279-282.
[7] 彭邦远, 张洪礼, 孙小静, 等.热处理刺梨汁香气物质的SPME-GC-MS检测与主成分分析[J].食品科学, 2018, 39(6):230-236.
PENG B Y, ZHANG H L, SUN X J, et al.Analysis of volatile aroma compounds of heated Rosa roxbuighii Tratt juice by solid-phase microextraction combined with gas chromatography-mass spectrometry and principal component analysis[J].Food Science, 2018, 39(6):230-236.
[8] 王雪雅, 丁筑红, 梁芳, 等.热处理条件对刺梨果汁风味物质和营养成分的影响研究[J].食品工业科技, 2016, 37(15):107-111.
WANG X Y, DING Z H, LIANG F, et al.Effects of heating treatment on the flavor compounds and the quality change of the Rosa roxbuighii Tratt juice[J].Science and Technology of Food Industry, 2016, 37(15):107-111.
[9] 周志, 汪兴平, 罗祖友, 等.野生刺梨汁中游离态和O-糖苷键合态挥发性物质的检测[J].食品科学, 2012, 33(14):192-197.
ZHOU Z, WANG X P, LUO Z Y, et al.Identification and analysis of free and O-glycoside-bound volatile components in wild Rosa roxburghii juice[J].Food Science, 2012, 33(14):192-197.
[10] NACCARATO A, PAWLISZYN J.Matrix compatible solid phase microextraction coating, a greener approach to sample preparation in vegetable matrices[J].Food Chemistry, 2016, 206:67-73.
[11] 蒋彰, 周志磊, 姬中伟, 等.即墨黄酒煮糜工艺对挥发性物质的影响[J].食品与发酵工业, 2020.https://doi.org/10.13995/j.cnki.11-1802/ts.025547.
JIANG Z, ZHOU Z L, JI Z W, et al.Effect of millet boiling process on volatile compounds in Jimo Huangjiu[J].Food and Fermentation Industries, 2020.https://doi.org/10.13995/j.cnki.11-1802/ts.025547.
[12] ENGEL W, BAHR W, SCHIEBERLE P.Solvent assisted flavor evaporation-a new and versatile technique for the careful and direct isolation of aroma compound from complex food matrices[J].European Food Research and Technology, 1999, 209(3-4):237-241.
[13] 宋焕禄. 分子感官科学[M].北京:科学出版社, 2014.
SONG H L.Molecular Sensory Science[M].Beijing:Science Press, 2014.
[14] 宫俐莉, 李安军, 孙金沅, 等.溶剂辅助风味蒸发法与顶空-固相微萃取法结合分析白酒酒醅中挥发性风味成分[J].食品与发酵工业, 2016, 42(9):169-177.
GONG L L, LI A J, SUN J Y, et al.Analysis on volatile flavor compounds of fermented grains by SAFE and HS-SPME coupled with GC-MS[J].Food and Fermentation Industries, 2016, 42(9):169-177.
[15] 李凯, 王金晶, 李永仙, 等.红心火龙果果酒特征香气分析[J].食品与发酵工业, 2019, 45(13):217-223.
LI K, WANG J J, LI Y X, et al.Characteristic aroma components of red pitaya wine[J].Food and Fermentation Industries, 2019, 45(13):217-223.
[16] MOSNDL A, GUENTHER C.Stereoisomeric flavor compounds.20.structure and properties of γ-lactone enantiomers[J].Journal of Agricultural and Food Chemistry, 1989, 37(2):413-418.
[17] SCHWAB W, DAVIDOVICH-RIKANATI R, LEWINSOHN E.Biosynthesis of plant-derived flavor compounds[J].Plant Journal, 2008, 54(4):712-732.
[18] BUETTBER A.施普林格气味手册[M].北京:科学出版社, 2019.
BUETTBER A.Springer Handbook of Odor[M].Beijing:Science Press, 2019.
[19] 李秋棉, 罗均, 李雪萍, 等.果实香气物质的合成与代谢研究进展[J].广东农业科学, 2012, 39(19):104-107.
LI Q M, LUO J, LI X P, et al.Advances in the study on the biosynthesis and metabolism of volatile compounds in fruits[J].Guangdong Agricultural Sciences, 2012, 39(19):104-107.
[20] WEIN M, LEWINSOHN E, SCHWAB W.Metabolic fate of isotopes during the biological transformation of carbohydrates to 2,5-dimethyl-4-hydroxy-3(2H)-furanone in strawberry fruits[J].Journal of Agricultural and Food Chemistry, 2001, 49(5):2 427-2 432.
[21] RAAB T, LÓPEZ-RÁEZ J A, KLEIN D, et al.FaQR, required for the biosynthesis of the strawberry flavor compound 4-hydroxy-2,5-dimethyl-3(2H)-furanone, encodes an enone oxidoreductase[J].Plant Cell, 2006, 18(4):1 023-1 037.
[22] SCHIEFNER A, SINZ Q, NEUMAIER I, et al.Structural basis for the enzymatic formation of the key strawberry flavor compound 4-hydroxy-2,5-dimethyl-3(2H)-furanone[J].Journal of Biological Chemistry, 2013, 288(23):16 815-16 826.
[23] LAVID N, SCHWAB W, KAFKAS E, et al.Aroma biosynthesis in strawberry:S-adenosylmethionine:furaneol O-methyltransferase activity in ripening fruits[J].Journal of Agricultural and Food Chemistry, 2002, 50(14):4 025-4 030.
[24] TRAPP S C, CROTEAU R B.Genomic organization of plant terpene synthases and molecular evolutionary implications[J].Genetics, 2001, 158(2):811-832.
[25] MAEDA H, DUDAREVA N.The shikimate pathway and aromatic amino acid biosynthesis in plants[J].Annual Review of Plant Biology, 2012, 63:73-105.
[26] MARTINS C, RUEFF J, RODRIGUES A S.Genotoxic alkenylbenzene flavourings, a contribution to risk assessment[J].Food and Chemical Toxicology, 2018, 118:861-879.
[27] 谢克林, 冯涛, 庄海宁, 等.外源可发酵糖对葡萄酒挥发性化合物的影响[J].食品科学, 2016, 37(18):113-119.
XIE K L, FENG T, ZHUANG H N, et al.Influence of different exogenous fermentable sugars on volatile compounds of wine[J].Food Science, 2016, 37(18):113-119.
[28] GEMERT L J V.Odour Thresholds-Compilations of Odour Threshold Values in Air, Water and Other Media[M].The Netherlands:Oliemans Punter & Partners BV, 2011.
[29] ZHU Y, LYU H P, SHAO C Y, et al.Identification of key odorants responsible for chestnut-like aroma quality of green teas[J].Food Research International, 2018, 108:74-82.
Outlines

/