The safety of strains 1-4 and 4-2, two potential probiotics isolated from the oropharynx of healthy children with broad-spectrum antagonism against Staphylococcus aureus and other common pathogenic bacteria, were evaluated so as to provide safety basis for the development and preparation of probiotics as respiratory tract products. The acute toxicity test and the determination of drug resistance of SPF mice with different doses of bacterial liquid containing strains 1-4 and 4-2 were conducted, and evaluation were made by observing death of the animals, signs of acute toxicity, bacterial translocation and growth, etc. Death of animals, or significant difference between the experimental group and the control group in weight change, food intake and organ index were not observed. Strains 1-4 and 4-2 were sensitive to 8 antibiotics tested and were resistant to cefotaxime and ceftriaxone, in addition strain 4-2 was also resistant to erythromycin. The safety evaluation of strains 1-4 and 4-2 showed that they were harmless to mice and had good safety, and could be used as backup strains for preparation of respiratory microecological and other products.
WANG Nan
,
TIAN Han
,
ZHANG Wenxiao
,
BAI Xiaocui
,
XIAO Chunling
. Safety evaluation of two strains of human Streptococcus with potential probiotics[J]. Food and Fermentation Industries, 2021
, 47(5)
: 12
-16
.
DOI: 10.13995/j.cnki.11-1802/ts.025289
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