In this study, HPLC-MS/MS technology was used to analyze the main components of lemon juice, and the antioxidant activity was investigated as well at cellular level. The results demonstrated that the main components of lemon juice were organic acid (quininic acid, malic acid, and citric acid, etc), amino acid (aspartic acid and serine, etc) and flavonoids (vitexin, naringin, and polydatin, etc). The antioxidant activity assay in cells showed that the treatment of lemon juice could promote the synthesis of mitochondrion and expression of SOD2. And it could also increase the activity of SOD, and consequently reduce the cellular ROS level and effectively improve palmitic acid-induced oxidative damage in L-02 hepatocyte. The result of this study will provide theoretical foundations for the enhancement the functional value of lemon juice.
KUANG Wenling
,
LI Jia
,
HAN Lin
,
JIANG Yongbo
,
QIU Linglan
,
WANG Kaituo
,
WANG Min
. Analysis of the main water-soluble components of lemon juice and its effect on palmitic acid-induced oxidative damage in L-02 hepatocyte[J]. Food and Fermentation Industries, 2021
, 47(5)
: 43
-47
.
DOI: 10.13995/j.cnki.11-1802/ts.025361
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