Food and Fermentation Industries >

2021 , Vol. 47 >Issue 6: 18 - 25

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025179

The study on stability of food-grade Pickering emulsion and the loading of β-carotene

  • FENG Xin ,
  • MA Liang ,
  • DAI Hongjie ,
  • FU Yu ,
  • YU Yong ,
  • ZHU Hankun ,
  • WANG Hongxia ,
  • ZHANG Yuhao
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  • 1(College of Food Science, Southwest University, Chongqing 400715, China)
    2(Biological Science Research Center, Academy for Advanced Interdisciplinary Studies, Southwest University, Chongqing 400715, China)
    3(Chongqing Key Laboratory of Soft-Matter Material Chemistry and Function Manufacturing, Chongqing 400715, China)

Received date: 2020-07-27

  Revised date: 2020-09-27

  Online published: 2021-04-15

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Abstract

The pH, ionic strength and thermal stability of food-grade Pickering emulsion based on gelatin were studied systematically, and three kinds of Pickering emulsion were used to realize the loading of β-carotene. The results showed that stable Pickering emulsion could hardly be formed at the near isoelectric point (pH 5). Increasing the electrostatic repulsion force of the GNPs dispersion was beneficial to the formation of uniform Pickering emulsion. Meanwhile, the addition of NaCl could increase droplet size and creaming (CI) due to electrostatic shielding. With the further increase of ionic strength (100-500 mmol/L), the droplet size and the CI showed a decrease and tend to be stable due to the improvement of the interaction among droplets. Furthermore, the droplet size and CI increased with the prolongation of heating time. The study of β-carotene loading showed that the high viscoelastic Pickering emulsion could significantly reduce the photo-degradation of β-carotene, and remained over 70% after 30 d of storage (4 ℃). Moreover, the high viscoelastic emulsion would significantly improve the bio-accessibility of β-carotene, which was more conducive to digestion and absorption in vivo.

Key words： food-grade; gelatin nanoparticles; Pickering emulsion; stability; β-carotene

Cite this article

FENG Xin , MA Liang , DAI Hongjie , FU Yu , YU Yong , ZHU Hankun , WANG Hongxia , ZHANG Yuhao . The study on stability of food-grade Pickering emulsion and the loading of β-carotene[J]. Food and Fermentation Industries, 2021 , 47(6) : 18 -25 . DOI: 10.13995/j.cnki.11-1802/ts.025179

References

[1] 谭天仪, 李璟, 夏锐, 等.超细化豆渣作为皮克林乳液稳定剂的特性研究[J].食品与发酵工业, 2020,46(2):47-54.
TAN T Y, LI J, XIA R, et al.Preparation of ultrafine okara and its characteristics as a stabilizer for Pickering emulsion[J].Food and Fermentation Industries, 2020, 46(2):47-54.
[2] 刘兴丽, 赵双丽, 肖乃勇, 等.马铃薯蛋白微凝胶对皮克林乳液乳化特性的影响[J].轻工学报, 2019,34(5):1-9.
LIU X L, ZHAO S L, XIAO N Y, et al.Effect of potato protein microgel on emulsifying properties of Pickering emulsion[J].Journal of Light Industry, 2019, 34(5):1-9.
[3] SU J, GUO Q, CHEN Y, et al.Characterization and formation mechanism of lutein pickering emulsion gels stabilized by β-lactoglobulin-gum arabic composite colloidal nanoparticles[J].Food Hydrocolloids, 2020,98:105276.
[4] LIU G, WANG Q, HU Z, et al.Maillard-reacted whey protein isolates and epigallocatechin gallate complex enhance the thermal stability of the pickering emulsion delivery of curcumin[J].Journal of Agricultural and Food Chemistry, 2019,67(18):5 212-5 220.
[5] 朱雨晴, 刘伟, 陈兴, 等.食品级皮克林乳液的稳定机制及稳定性研究进展[J].食品工业科技, 2018,39(7):315-322.
ZHU Y Q, LIU W, CHEN X, et al.Advances in stabilization mechanism and stability control of food grade Pickering emulsion[J].Science and Technology of Food Industry, 2018, 39(7):315-322.
[6] 刘洋.大豆蛋白纳米颗粒稳定的乳液及其油凝胶性质[D].无锡:江南大学, 2016.
LIU Y.Properties of emulsions stabilized with soy protein nanoparticles and their oleogels[D].Wuxi:Jiangnan University, 2016
[7] DESTRIBATS M, ROUVET M, GEHIN-DELVAL C, et al.Emulsions stabilised by whey protein microgel particles:Towards food-grade Pickering emulsions[J].Soft Matter, 2014,10(36):6 941-6 954.
[8] ZHU X, ZHENG J, LIU F, et al.Freeze-thaw stability of Pickering emulsions stabilized by soy protein nanoparticles.Influence of ionic strength before or after emulsification[J].Food Hydrocolloids, 2018,74:37-45.
[9] DEGNER B M, CHUNG C, SCHLEGEL V, et al.Factors influencing the freeze-thaw stability of emulsion-based foods[J].Comprehensive Reviews in Food Science and Food Safety, 2014,13(2):98-113.
[10] WEI Z, HUANG Q.Developing organogel-based Pickering emulsions with improved freeze-thaw stability and hesperidin bioaccessibility[J].Food Hydrocolloids, 2019,93:68-77.
[11] ZHU Y, MCCLEMENTS D J, ZHOU W, et al.Influence of ionic strength and thermal pretreatment on the freeze-thaw stability of Pickering emulsion gels[J].Food Chemistry, 2020,303:125 401.
[12] GE S, XIONG L, LI M, et al.Characterizations of Pickering emulsions stabilized by starch nanoparticles:Influence of starch variety and particle size[J].Food Chemistry, 2017,234:339-347.
[13] 米雪, 刘树兴, 曾桥, 等.响应面法优化超声辅助提取柿皮类胡萝卜素及其体外抗氧化性研究[J].中国调味品, 2019,44(11):26-31.
MI X, LIU S X, ZENG Q, et al.Optimization of ultrasound-assisted extraction of carotenoids from persimmon peel by response surface methodology and its antioxidant activity in vitro[J].China Condiment, 2019, 44(11):26-31.
[14] LU Y, MAO L, ZHENG H, et al.Characterization of β-carotene loaded emulsion gels containing denatured and native whey protein[J].Food Hydrocolloids, 2020,102:105 600.
[15] RODRIGUES R M, RAMOS P E, CERQUEIRA M F, et al.Electrosprayed whey protein-based nanocapsules for β-carotene encapsulation[J].Food Chemistry, 2020,314:126 157.
[16] LIN Q, LIANG R, ZHONG F, et al.Effect of degree of octenyl succinic anhydride (OSA) substitution on the digestion of emulsions and the bioaccessibility of β-carotene in OSA-modified-starch-stabilized-emulsions[J].Food Hydrocolloids, 2018,84:303-312.
[17] CHEN L, YOKOYAMA W, LIANG R, et al.Enzymatic degradation and bioaccessibility of protein encapsulated β-carotene nano-emulsions during in vitro gastro-intestinal digestion[J].Food Hydrocolloids, 2020,100:105 177.
[18] DAI L, SUN C, WEI Y, et al.Characterization of Pickering emulsion gels stabilized by zein/gum arabic complex colloidal nanoparticles[J].Food Hydrocolloids, 2018,74:239-248.
[19] MAO L, MIAO S, YUAN F, et al.Study on the textural and volatile characteristics of emulsion filled protein gels as influenced by different fat substitutes[J].Food Research International, 2018,103:1-7.
[20] ROOHINEJAD S, OEY I, WEN J, et al.Formulation of oil-in-water β-carotene microemulsions:Effect of oil type and fatty acid chain length[J].Food Chemistry, 2015,174:270-278.
[21] FENG X, DAI H, MA L, et al.Food-grade gelatin nanoparticles:preparation, characterization, and preliminary application for stabilizing pickering emulsions[J].Foods, 2019,8(10):479.
[22] FENG X, DAI H, MA L, et al.Properties of Pickering emulsion stabilized by food-grade gelatin nanoparticles:Influence of the nanoparticles concentration[J].Colloids and Surfaces B:Biointerfaces, 2020.196:111 294.
[23] COESTER C J,LANGER K,VON BRIESEN H,et al.Gelatin nanoparticles by two step desolvation a new preparation method, surface modifications and cell uptake[J].Journal of Microencapsulation, 2000,17(2):187-193.
[24] LIU F, TANG C.Soy glycinin as food-grade Pickering stabilizers:Fabrication of gel-like emulsions and their potential as sustained-release delivery systems for β-carotene[J].Food Hydrocolloids, 2016,56:434-444.
[25] YUAN Y, GAO Y, ZHAO J, et al.Characterization and stability evaluation of β-carotene nanoemulsions prepared by high pressure homogenization under various emulsifying conditions[J].Food Research International, 2008,41(1):61-68.
[26] TAN H, ZHAO L, TIAN S, et al.Gelatin particle-stabilized high-internal phase emulsions for use in oral delivery systems:protection effect and in vitro digestion study[J].Journal of Agricultural and Food Chemistry, 2017,65(4):900-907.
[27] LIU W, WANG J, MCCLEMENTS D J, et al.Encapsulation of β-carotene-loaded oil droplets in caseinate/alginate microparticles:Enhancement of carotenoid stability and bioaccessibility[J].Journal of Functional Foods, 2018,40:527-535.
[28] 王启明, 唐瑜婉, 杨雅轩, 等.pH对麦醇溶蛋白/槲皮素相互作用及其Pickering乳液特性的影响[J].食品科学, 2020,41(20):27-34.
WANG Q M, TANG Y W, YANG Y X, et al.Effects of pH on the interaction of wheat gliadin/quercetin and pickering emulsion properties[J].Food Science, 2020,41(20):27-34.
[29] 张亚珍, 熊文飞, 裴亚琼, 等.盐离子对花生粕分离蛋白纳米粒子及其稳定Pickering乳液特性的影响[J].食品科学, 2019,40(24):1-7.
ZHANG Y Z, XIONG W F,PEI Y Q, et al.Effects of salt ions on the properties of peanut protein isolate nanoparticles and pickering emulsion stabilized with the nanoparticles[J].Food Science, 2019,40(24):1-7.
[30] LIMA CARDIAL M R, PAULA H C B, DA SILVA R B C, et al.Pickering emulsions stabilized with cashew gum nanoparticles as indomethacin carrier[J].International Journal of Biological Macromolecules, 2019,132:534-540.
[31] DAI H, LI Y, MA L, et al.Fabrication of cross-linked β-lactoglobulin nanoparticles as effective stabilizers for Pickering high internal phase emulsions[J].Food Hydrocolloids, 2020,109:106 151.
[32] KASIRI N, FATHI M.Production of cellulose nanocrystals from pistachio shells and their application for stabilizing Pickering emulsions[J].International Journal of Biological Macromolecules, 2018,106:1 023-1 031.
[33] LIU W, GAO H, MCCLEMENTS D J, et al.Stability, rheology, and β-carotene bioaccessibility of high internal phase emulsion gels[J].Food Hydrocolloids, 2019,88:210-217.
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