Food and Fermentation Industries >

2021 , Vol. 47 >Issue 6: 221 - 227

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025494

Detection of higher alcohols content in turbid rice wine by the colorimetric method of microplate reader

  • YUAN Guoyi ,
  • WANG Chunxiao ,
  • HE Yulin ,
  • QIU Shuyi
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  • Province Key Laboratory of Fermentation Engineering and Biopharmacy, School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China

Received date: 2020-08-27

  Revised date: 2020-11-05

  Online published: 2021-04-15

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Abstract

The detection of higher alcohols content in turbid rice wine is very important for measuring its quality. The aim of this study is to explore the applicability of the colorimetric method using microplate reader for the detection of higher alcohols content in turbid rice wine. Single and mixed standard curves of six higher alcohols (isobutanol, isoamyl alcohol, phenylethanol, n-propanol, n-butanol, hexanol) were established for their detection in turbid rice wine using the colorimetric method of microplate reader. The contents of higher alcohols in turbid rice wine were examined and compared between the colorimetric method of microplate reader and gas chromatography method. The results showed that linearity of mixed standard curves was reliable in the range of 0-0.05 mg, and the linearity of a single standard curve of n-propanol was worse in the range of 0-1 mg, whereas the linearity of phenylethanol and n-butanol was not good in the range of 0-0.05 mg. The content of n-propanol, phenylethanol, and n-butanol would affect the detection of other higher alcohols to a certain extent. The mass concentration of six higher alcohols in rice wine fermented by FBKL2.8022 and FBKL2.8023 was examined to be 408.94 and 230.89 mg/L, respectively. The standard deviation range was 0.06%-3.94% in the rice wine fermented by FBKL2.8022, and 0.72%-1.58% in the rice wine fermented by FBKL2.8023. The content of higher alcohols examined by the colorimetric method of microplate reader was generally higher than the gas chromatography method. Different ratios of higher alcohols would affect the linear equation and correlation coefficient of standard curve established in the detection of higher alcohols in turbid rice wine using colorimetric method of microplate reader. When analyzing sample, it is necessary to select the standard curve with appropriate ratios of different higher alcohols base on the sample detection requirements.

Key words： rice wine; higher alcohols; fermentation; glutinous rice; gas chromatography; isobutanol; isoamylo

Cite this article

YUAN Guoyi , WANG Chunxiao , HE Yulin , QIU Shuyi . Detection of higher alcohols content in turbid rice wine by the colorimetric method of microplate reader[J]. Food and Fermentation Industries, 2021 , 47(6) : 221 -227 . DOI: 10.13995/j.cnki.11-1802/ts.025494

References

[1] WEI X L,LIU S P,YU J S,et al.Innovation Chinese rice wine brewing technology by bi-acidification to exclude rice soaking process[J].Journal of Bioscience & Bioengineering,2016,123(4):460-465.
[2] WANG Q J,SUN D W,JEONG S,et al.Screening of rice cultivars for brewing high quality turbid rice wine[J].LWT-Food Science and Technology,2014,56:145-152.
[3] LEE J,LEE Y,HA J,et al.Simultaneous determination of four bioactive compounds in Korean rice wine (makgeolli) by solvent extraction coupled with gas chromatography-mass spectrometry[J].International Journal of Food Properties,2018,21(1):139-146.
[4] 王春晓,唐佳代,吴鑫颖, 等.酿酒小曲中功能微生物的研究进展[J].食品科学,2019,40(17):309-316.
WANG C X,TANG J D,WU X Y,et al.Progress in research of functional microbes in xiaoqu,a Chinese traditional fermentation starter for liquor and rice wine[J].Food Science,2019,40(17):309-316.
[5] 黄桂东,徐珊珊,刘惠,等.降黄酒高级醇无机氮源的筛选[J].食品与生物技术学报,2018,37(1):82-87.
HUANG G D,XU S S, LIU H,et al.Selected inorganic nitrogen in reducing the contents of higher alcohols from Chinese rice wine[J].Food Science and Biotechnology,2018,37(1):82-87.
[6] 董洋,刘亚琼,刘姗姗.杂粮红曲酒高级醇及其挥发性风味物质研究[J].酿酒科技,2017(3):55-59.
DONG Y,LIU Y Q,LIU S S.Higher alcohols and volatile flavoring substances in monascus yellow rice wine produced by miscellaneous grains[J].Liquor-Making Science & Technology,2017(3):55-59.
[7] CHEN S,XU Y,QIAN M C.Aroma characterization of chinese rice wine by gas chromatography-olfactometry, chemical quantitative analysis, and aroma reconstitution[J].Journal of Agricultural and Food Chemistry, 2013,61(47):11 295-11 302.
[8] WANG P X,MAO J,MENG X Y,et al.Changes in flavour characteristics and bacterial diversity during the traditional fermentation of Chinese rice wines from Shaoxing region[J].Food Control,2014, 44:58-63.
[9] 苟静瑜,贾智勇,闫宗科, 等.降低白酒中高级醇含量的研究进展[J].酿酒,2016,3(4):25-29.
GOU J Y,JIA Z Y,YAN Z K,etal.Research progress in decreasing the contents of higher alcohols in baijiu(liguor)[J].Liquor Making,2016,3(4):25-29.
[10] 黄桂东, 吴子蓥, 唐素婷, 等.黄酒中高级醇含量控制与检测研究进展[J].中国酿造, 2018,37(1):7-11.
HUANG G D,WU Z Y,TANG S T,et al.Research progress on control and detection of higher alcohols contents from Chinese rice wine[J].China Brewing,2018,37(1):7-11.
[11] 王珍,周洋,李春丽,等.浅谈白酒中杂醇油的分析方法[J].酿酒科技,2017(6):77-79.
WANG Z,ZHOU Y, LI C L,etal.Analytic methods of fusel oil in baijiu[J].Liquor-Making Science & Technology,2017(6):77-79.
[12] 唐开永,周鸿翔,刘彩婷,等.米酒中6种醇的气相色谱外标法测定研究[J].中国酿造, 2019,38(4):183-187.
TANG K Y,ZHOU H X,LlU C T,etal.Determination of 6 alcohols in rice wine by GC external standard method[J].China Brewing,2019,38(4):183-187.
[13] 汤二将.高级醇毒性效应及其定量构效关系研究[D].福建师范大学,2013.
TANG E J.Higher alcohols toxicity effect and quanti-tative structure activity relationship study[D].Fujian Normal University, 2013.
[14] 卫生部. GB/T 5009.48—2003 蒸馏酒与配制酒卫生标准的分析方法[S].北京:中国标准出版社,2003.
Ministry of health. GB/T 5009.48—2003 Analysis method of hygienic standard for distilled liquor and mixed liquor[S].Beijing:China Standard Press,2003
[15] 国家质量监督检验检疫总局. GB/T 394.2—2008 酒精通用分析方法[S].北京:中国标准出版社,2008.
General administration of quality supervision, inspection and quarantine of the people's Republic of China. GB/T 394.2—2008 General analysis method of alcohol[S].北京:中国标准出版社,2003.
[16] 梁慧,薛正莲,周扬,等.酶标仪法测定γ-氨基丁酸[J].食品与发酵工业,2014,40(7):156-160.
LIANG H,XUE Z L, ZHOU Y,etal.Optimization of the determination of γ-aminobutyric acid by microplate reader[J].Food and fermentation industries,2014,40(7):156-160.
[17] 冉旭东,吴远根.百草枯核酸适配体的筛选及其比色检测研究[J].分析化学,2019,47(4):567-575.
RAN X D,WU Y G.Screening aptamers and development of colorimetric detection method of paraquat pesticide[J].Chinese Journal of Analytical Chemistry,2019,47(4):567-575.
[18] 石彬,李咏富,吴远根.氯化血红素比色法检测乳制品中土霉素[J].中国酿造,2018,37(7):168-172.
SHI B,LI Y F,WU Y G.Determination of oxytetracycline in dairy products by hemin colorimetry[J].China Brewing,2018,37(7):168-172.
[19] 石耀强,张晗依,刘双月.酶标仪检测技术应用的研究进展[J].科技展望,2016,26(24):73;107.
SHI Y Q,ZHANG H Y,LIU S Y.Research progress in the application of microplate reader testing technology[J].Science and Technology,2016,26(24):73;107.
[20] WANG C X,TANG J D,QIU S Y.Profiling of fungal diversity and fermentative yeasts in traditional Chinese Xiaoqu[J].Frontiers in Microbiology,2020,11:2 103.
[21] 范衍琼.分光光度法测定蒸馏酒及配制酒中杂醇油[J].光谱实验室,2005(2):304-306.
FAN Y Q.Determination of fusel oil in distilled wines and mixed wines by spectrophotometry[J].Guangpu Shiyanshi,2005(2):304-306.
[22] 穆文斌,刘国英,郭增,等.杂醇油测定误差原因和改进措施[J].酿酒科技,2004(2):87-88.
MU W B,LlU G Y,GUO Z,et al.Causations for the errors in the determination of fusel oiland the relative improvement measures[J].Liquor-Making Science & Technology,2004(2):87-88.
[23] 汪勇,王海龙.比色法测定啤酒中高级醇含量[J].啤酒科技,2008(8):48-49.
WANG Y,WANG H L.Determination of higher alcohols in beer by colorimetry[J].Beer Technology,2008(8):48-49.
[24] 杜威,林玲,张兴亚,等.分光光度法测定黄酒中的高级醇[J].酿酒科技,2011(7):105-107.
DU W,LIN L,ZHANG X Y,etal.Determination of higher alcohols content in yellow rice wine by spectrophotometry[J].Liquor-Making Science & Technology,2011(7):105-107.
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