Effect of sea cucumber enzymatic hydrolysate on skin wound healing in SD rats

  • CHENG Minjun ,
  • HU Jinhua ,
  • TANG Xue ,
  • YANG Tingting ,
  • ZHOU Peng ,
  • HU Wei
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  • 1(State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China)
    2(School of Food Science and Technology,Jiangnan University,Wuxi 214122,China)
    3(Shandong Homey Aquatic Development Co.Ltd.,Weihai 264200,China)

Received date: 2020-10-18

  Revised date: 2020-11-08

  Online published: 2021-05-20

Abstract

The impact of sea cucumber hydrolysate (SCH) on skin wound healing in SD rats was investigated, aiming to provide basis for the application of SCH in wound healing.A rat model of full-thickness cutaneous wounds was established in a model group, three SCH dose groups and a positive group, and the rats were sacrificed in batches on the 4th, 8th and 12nd day after surgery.The effect of SCH on wound healing was comprehensively analyzed in combination with the results of wound healing rate, observation of tissues stained with hematoxylin-eosin (H&E) and Masson’s trichrome method (Masson), changes of interleukin-8 (IL-8) and interleukin-10 (IL-10) in the blood, detection of hydroxyproline (Hyp), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) in wounds.Results showed that the wound healing rate of each dose group of SCH was faster than that of the model group.H&E results showed that SCH could regulate the infiltration of inflammatory cells and promote angiogenesis, and the epidermis recovered fastest in the medium dose group.Based on the results of IL-8 and IL-10, SCH could regulate their secretion to reduce the inflammatory reaction duration.Collagen fibers formation in the wound significantly promoted by SCH was observed in Masson’s trichrome stained tissues, and Hyp content increased accordingly.In addition, the mechanism of SCH wound healing may be related to the significant increase of growth factors secretion.The prepared SCH could effectively promote wound healing in SD rats, with best performance observed in the medium dose group (0.50 g/kg).

Cite this article

CHENG Minjun , HU Jinhua , TANG Xue , YANG Tingting , ZHOU Peng , HU Wei . Effect of sea cucumber enzymatic hydrolysate on skin wound healing in SD rats[J]. Food and Fermentation Industries, 2021 , 47(7) : 94 -101 . DOI: 10.13995/j.cnki.11-1802/ts.025916

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