Food and Fermentation Industries >

2021 , Vol. 47 >Issue 7: 244 - 250

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025869

Detection of pefloxacin residue in milk based on aptamer recognizing and time-resolved fluorescence resonance energy transfer assay

  • SONG Yaning ,
  • HU Chaoqiong ,
  • HUO Qiuyu ,
  • WANG Lijun ,
  • CHEN Xianggui ,
  • HUANG Yukun
Expand
  • 1(School of Food and Bioengineering,Xihua University,Chengdu 610039,China)
    2(Key Laboratory of Food Non Thermal Processing,Engineering Technology Research Center of Food Non Thermal Processing,Yibin Xihua University Research Institute,Yibin 644004,China)

Received date: 2020-10-13

  Revised date: 2020-12-04

  Online published: 2021-05-20

Fold

Abstract

Pefloxacin (PEF) is widely used in the prevention and treatment of livestock and poultry diseases,which is frequently causing veterinary drug residues in food and thus affecting human health.Therefore,it is necessary to construct an effective and rapid detection method for PEF.NaYF4:Ce/Tb nanomaterial with time-resolved fluorescence properties was synthesized by using the solvent hot method in this study.Simultaneously,complementary strand (cDNA) of PEF aptamer was designed by computer aided molecular docking.Gold nanoparticles and luminescent nanomaterials were modified onto aptamer and cDNA respectively.Thereby time-resolved fluorescence resonance energy transfer (TR-FRET) between these two nanomaterials was induced in case of complementary base pairing.Based on this principle,the novel detection method of PEF in milk was constructed.Under the optimal experimental conditions,the linear range of detection was 0.2-20 μ/kg (y=1 567.3x+3 956.3,R2=0.974 2).The recovery rates were 73.81%-99.86%.These results showed that the present method can be applied in the detection of PEF in the practical milk sample.

Key words： pefloxacin; time-resolved fluorescence; aptamer; milk

Cite this article

SONG Yaning , HU Chaoqiong , HUO Qiuyu , WANG Lijun , CHEN Xianggui , HUANG Yukun . Detection of pefloxacin residue in milk based on aptamer recognizing and time-resolved fluorescence resonance energy transfer assay[J]. Food and Fermentation Industries, 2021 , 47(7) : 244 -250 . DOI: 10.13995/j.cnki.11-1802/ts.025869

References

[1] LI S,CHOE W S.Screening of pefloxacin-binding single strand DNA aptamer[J].Journal of Biotechnology,2008,136:S86.
[2] 李宵宁,柴芸,刘明亮.喹诺酮类抗菌药的作用机制及耐药机制研究进展[J].国外医药(抗生素分册),2015,36(3):97-102.
LI X N,CHAI Y,LIU M L.Research progress on action mechanism and resistance mechanism of quinolone antimicrobials[J].World Notes on Antibiotics,2015,36(3):97-102.
[3] ZHU M F,LI R,LAI M S,et al.Copper nanoparticles incorporating a cationic surfactant-graphene modified carbon paste electrode for the simultaneous determination of gatifloxacin and pefloxacin[J].Journal of Electroanalytical Chemistry,2020,857:113 730.
[4] 中华人民共和国农业部.中华人民共和国农业部公告 第2292号[EB/OL].[2015-09-07] http://www.moa.gov.cn/gk/tzgg_1/gg/201509/t20150907_4819267.htm
Ministry of Agriculture of the People’s Republic of China.Notice No.2292 of the Ministry of Agriculture of the People’s Republic of China[EB/OL].[2015-09-07] http://www.moa.gov.cn/gk/tzgg_1/gg/201509/t20150907_4819267.htm
[5] 王远丽.对畜产品质量安全检测需求的思考[J].中国畜牧业,2019(12):68.
WANG Y L.Thoughts on the demand of quality and safety inspection of animal products[J].China Animal Husbandry,2019 (12):68.
[6] DANG P K,DEGAND G,DOUNY C,et al.Optimisation of a new two-plate screening method for the detection of antibiotic residues in meat[J].International Journal of Food Science and Technology,2011,46(10):2 070-2 076.
[7] 曹敬政,安静,蒋蔚,等.直接竞争化学发光酶免疫法检测十种氟喹诺酮类药物方法的建立[J/OL].中国动物传染病学报:1-16.http://kns.cnki.net/kcms/detail/31.2031.S.20200310.1050.004.html.
CAO J Z,AN Jing,Jiang W,et al.Establishment of a direct competitive chemiluminescence enzyme immunoassay for the detection of ten fluoroquinolones [J/OL].Chinese Journal of Animal Infectious Diseases: 1-16.http://kns.cnki.net/kcms/detail/31.2031.S.20200310.1050.004.html.
[8] YUAN M F,XIONG Q R,ZHANG G G,et al.Silver nanoprism-based plasmonic ELISA for sensitive detection of fluoroquinolones[J].Journal of Materials Chemistry.B,2020,8(16):3 667-3 675.
[9] WU Y C,GUO S,DONG Q,et al.Development of an immunochromatographic test strip for rapid simultaneous detection of enrofloxacin and ofloxacin in tissue of chicken muscle and pork[J].Food Analytical Methods,2016,9(10):2 807-2 813.
[10] 王婷,陈明涛.基于多反应监测模式下的超高效液相色谱-串联质谱法检测动物源性食品中19种氟喹诺酮类抗生素残留[J].食品安全导刊,2020(7):66-70.
WANG T,CHEN M T.Detection of 19 fluoroquinolones in animal food by ultra high performance liquid chromatography-tandem mass spectrometry based on multi-reaction monitoring mode[J].China Food Safety Magazine,2020(7):66-70.
[11] TIAN Y P,JIA J H,HE J J,et al.Simultaneous detection of 46 veterinary drug residues in animal meat by UHPLC[J].Chromatographia,2016,79(7-8):457-471.
[12] 才凤,赵飞,贾宏新.超高效液相-串联质谱法测定速冻调理肉制品中18种喹诺酮类药物残留量[J].食品安全质量检测学报,2019,10(24):8 456-8 461.
CAI F,ZHAO F,JIA H X.Determination of 18 quinolones residues in quick-frozen meat products by ultra-high performance liquid chromatography-tandem mass spectrometry[J].Journal of Food Safety & Quality,2019,10(24):8 456-8 461.
[13] 张今君,夏慧丽,高海波.分散固相萃取-超高效液相色谱-串联质谱法测定豆芽中6种喹诺酮类药物[J].食品工业科技,2020,41(23):260-265;271.
ZHANG J J,XIA H L,GAO H B.Determination of 6 kinds of fluoroquinolones in bean sprouts based on dispersed solid phase extraction UPLC-MS/MS method[J].Science and Technology of Food Industry,2020,41(23):260-265,271.
[14] HU X B,GOUD K Y,KUMAR V S,et al.Disposable electrochemical aptasensor based on carbon nanotubes-V2O5-chitosan nanocomposite for detection of ciprofloxacin[J].Sensors and Actuators B-Chemical,2018,268:278-286.
[15] PAGANI A P,IBANEZ G A.Analytical approach for the simultaneous determination of quinolones in edible animal products.Modeling pH-modulated fluorescence excitation-emission matrices four-way arrays[J].Talanta,2019,192:52-60.
[16] LU W J,JIAOY,CAO Y F,et al.Bright yellow fluorescent carbon dots as a multifunctional sensing platform for the label-free detection of fluoroquinolones and histidine[J].Acs Applied Materials & Interfaces,2018,10(49):42 915-42 924.
[17] 朱俊,李叶平,邹金汕,等.碳点与曙红B间的荧光共振能量转移效应测定培氟沙星[J].光谱学与光谱分析,2019,39(8):2 554-2 560.
ZHU J,LI Y P,ZOU J S,et al.Determination of pefloxacin by fluorescence resonance energy transfer effect between carbon point and eosin B[J].Spectroscopy and Spectral Analysis,2019,39(8):2 554-2 560.
[18] 田红静,刘通,王秀娟,等.动物源性食品中抗生素残留的快速检测方法[J].食品安全质量检测学报,2020,11(11):3 391-3 397.
TIAN H J,LIU T,WANG X J,et al.Rapid methods for the detecting of antibiotic residues in animal-derived food[J].Journal of Food Safety & Quality,2020,11(11):3 391-3 397.
[19] 李嘉慧,刘凤银,王宇,等.食品中喹诺酮类抗生素多残留同时检测研究[J].生物化工,2019,5(4):149-151.
LI J H,LIU F Y,WANG Y,et al.Progress in simultaneous detection of quinolone antibiotic residues in food[J].Biological Chemical Engineering,2019,5(4):149-151.
[20] 白文荟,陈爱亮.适配体传感器在农药残留检测中的应用[J].分析测试学报,2016,35(10):1 360-1 368.
BAI W H,CHEN A L.Applications of aptasensor in determination for pesticide residues[J].Journal of Instrumental Analysis,2016,35(10):1 360-1 368.
[21] YIN Z Q,CHAI T T,MU P Q,et al.Multi-residue determination of 210 drugs in pork by ultra-high-performance liquid chromatography-tandem mass spectrometry[J].Journal of Chromatography A,2016,1 463:49-59.
[22] XU G H,ZHAO J J,LIU N,et al.Structure-guided post-SELEX optimization of an ochratoxin A aptamer[J].Nucleic Acids Research,2019,47(11):5 963-5 972.
[23] 张敏,张先舟,李聪,等.黄曲霉毒素B1核酸适配体筛选和检测方法的建立[J].食品科学,2020,41(24):295-303.
ZHANG M,ZHANG X Z,LI C,et al.Screening of aptamers for aflatoxin B1 and establishment of a method for aflatoxin B1 detection[J].Food Science,2020,41(24):295-303.
[24] 卢军利, 葛玮东,孙建霞,等.核酸适配体技术在食品有害物检测中的研究进展[J].中国农业科技导报,2015,17(2):151-158.
LU J L,GE W D,SUN J X,et al.Research progress of aptamer in detecting harmful substances in food[J].Journal of Agricultural Science and Technology,2015,17(2):151-158.
[25] 王巍,贾凌云.适配体筛选方法研究进展[J].分析化学,2009,37(3):454-460.
WANG W,JIA L Y.Progress in aptamer screening methods[J].Chinese Journal of Analytical Chemistry,2009,37(3):454-460.
[26] 樊晓博,谢兰心.酶标抗原直接竞争ELISA检测食品中氟喹诺酮类药物多残留[J].食品科学,2015,36(24):265-269.
Fan X B,XIE L X.Detection of fluoroquinolones residues in food by enzyme-labeled antigen direct competitive ELISA [J].Food science,2015,36(24):265-269.
[27] CHEN J H,LU N,SHEN X,et al.Investigation of an immunoassay with broad specificity to quinolone drugs by genetic algorithm with linear assignment of hypermolecular alignment of data sets and advanced quantitative structure-Activity relationship analysis[J].Journal of Agricultural and Food Chemistry,2016,64(13):2 772-2 779.
[28] 赵秋伶, 张振宇,周广原,等.恩诺沙星酶联适配体分析检测试剂盒的研制[J].分析科学学报,2018,34(5):644-648.
ZHAO Q L,ZHANG Z Y,ZHOU G Y,et al.Preparation of enzyme-linked aptamer assay kit for determining enrofloxacin residues[J].Journal of Analytical Science,2018,34(5):644-648.
[29] YU W L,LIU M X,LIU R B,et al.Development of biomimetic enzyme-linked immunosorbent assay based on molecular imprinting technique for semicarbazide detection[J].Food and Agricultural Immunology,2020,31(1):17-32.
[30] 周晓彤. 基于核酸适配体的氧氟沙星检测方法研究[D].上海:上海交通大学,2019.
ZHOU X T.The detetion methods for ofloxacin based on aptamer [D].Shanghai:Shanghai Jiaotong University,2019.
[31] REINEMANN C,FREIION V F U,RUDOLPH S,et al.Generation and characterization of quinolone-specific DNA aptamers suitable for water monitoring[J].Biosensors & Bioelectronics,2016,77:1 039-1 047.
[32] ZUKER M.Mfold web server for nucleic acid folding and hybridization prediction[J]Nucleic Acids Research,2003,31(13):3 406-3 415.
[33] TU D T,LIU L Q,JU Q,et al.Time-resolved fret biosensor based on amine-functionalized lanthanide-doped NaYF4 nanocrystals[J].Angewandte Chemie-International Edition,2011,50(28):6 306-6 310.
[34] HUANG Y K,WANG C,HUO Q Y,et al.A time-resolved luminescence aptasensor of ofloxacin based on rolling circle amplification and magnetic separation[J].Analytical and Bioanalytical Chemistry,2020,412(19):4 555-4 563.
[35] 史艳艳,高琳,李俊,等.液相色谱-串联质谱法检测饲料中洛美沙星、培氟沙星、氧氟沙星、诺氟沙星残留[J].食品安全质量检测学报,2019,10(11):3 367-3 375.
SHI Y Y,GAO L,LI J,et al.Determination of lomefloxacin,pefloxacin,ofloxacin and norfloxacin residues in feed by liquid chromatography-tandem mass spectrometry[J].Journal of Food Safety & Quality,2019,10(11):3 367-3 375.
[36] 宗婧婧,张小军,严忠雍,等.胶体金免疫层析法检测水产品中15种喹诺酮类药物[J].理化检验-化学分册,2018,54(5):591-595.
ZONG J J,ZHANG X J,YAN Z Y,et al.Detection of 15 quinolones in aquatic products by colloidal gold immunochromatography assay[J].Physical Testing and Chemical Analysis (Part B: Chemical Analysis),2018,54(5):591-595.
[37] 姜金庆,杨雪峰,王自良,等.氟喹诺酮类药物多残留间接竞争ELISA检测方法的建立[J].中国预防兽医学报,2011,33(11):887-892.
JIANG J Q,YANG X F,WANG Z L,et al.Development of indirect competitive ELISA for detection of fluoroquinolone residues[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(11):887-892.
[38] 李周敏,李心爱,姚开安,等.可视化蛋白芯片法同时检测牛奶中喹诺酮类抗生素残留的含量[J].药物分析杂志,2019,39(6):1 139-1 147.
LI Z M,LI X A,YAO K A,et al.Visual protein chip method for simultaneous determination of quinolone antibiotic residues in milk[J].Chinese Journal of Pharmaceutical Analysis,2019,39(6):1 139-1 147.
Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.