Food and Fermentation Industries >

2021 , Vol. 47 >Issue 8: 6 - 13

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025804

Synergetic regulation of gut microbiota by dietary fiber and phenolic compounds in hulless barley

  • LU Chaofeng ,
  • HUANG Jiaqi ,
  • HUANG Yonghua ,
  • YANG Shihua ,
  • CHEN Bi ,
  • YANG Mingjing ,
  • LI Yongqiang
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  • 1(College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China)
    2(Hangzhou Hanku Medical Laboratory Limited Company, Hangzhou 310000, China)
    3(West Yunnan University of Applied Sciences College of Tea(Pu'er), Pu'er 665099, China)
    4(College of Foreign Languages, Yunnan Agricultural University, Kunming 650201, China)

Online published: 2021-05-20

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Abstract

In this study, hulless barley flour (HBF), insoluble dietary fiber-phenolic compounds (IDF-PC) and soluble dietary fiber-phenolic compounds (SDF-PC) were used as experimental materials to analyze the synergetic regulation of gut microbiota by dietary fiber and polyphenol, according to the abundance and diversity of microbes by in vitro colonic fermentation and 16S rRNA high-throughput sequencing. Ferulic acid (FA) was taken as the control. IDF-PC and SDF-PC were extracted and isolated by enzymatic hydrolytic methods. The total phenolic content was determined using Folin-Ciocalteu assay. Moreover, short-chain fatty acids were analyzed by high performance liquid chromatography. The results showed that HBF, IDF-PC and SDF-PC were rich in dietary fibers and phenolic compounds. After in vitro colonic fermentation, these three samples could improve the abundance and diversity of gut microbiota and promote the growth of bacterial groups, including Lactobacillus, Akkermansia, Ruminococcaceae-UCG-005, Ruminococcaceae-NK4A214-group, compared with FA. Fermentation of HBF, IDF-PC and SDF-PC resulted in an increase in SCFAs production and the peak contents in acetic acid, which were (0.29±0.21), (0.15±0.05) and (0.14±0.10) mg/mL, respectively. This study suggests that hulless barley dietary fibers and phenolic compounds have better synergistic regulation on gut microbiota, which can provide scientific basis for hulless barley health promotion and disease prevention.

Key words： hulless barley; dietary fiber; phenolics; in vitro colonic fermentation; gut microbiota; synergetic regulation

Cite this article

LU Chaofeng , HUANG Jiaqi , HUANG Yonghua , YANG Shihua , CHEN Bi , YANG Mingjing , LI Yongqiang . Synergetic regulation of gut microbiota by dietary fiber and phenolic compounds in hulless barley[J]. Food and Fermentation Industries, 2021 , 47(8) : 6 -13 . DOI: 10.13995/j.cnki.11-1802/ts.025804

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