Food and Fermentation Industries >

2021 , Vol. 47 >Issue 8: 101 - 107

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025382

Identification and antimicrobial activity of the rare Actinomyces sp.from mangrove sediment

  • ZHANG Junliang ,
  • ZHANG Shiling ,
  • WU Jiahui ,
  • XU Shanshan ,
  • LIANG Jinyou ,
  • XU Ying
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  • 1(Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China)
    2(Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China)
    3(College of Engineering, China Pharmaceutical University, Nanjing 211198, China)

Online published: 2021-05-20

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Abstract

In this study, we reported the identification and antimicrobial activity of the rare actinomycete XY-R10 collected from the sediment of mangrove in Mai Po wetlands, Hong Kong. Actinobacteria were isolated on R2A plate and purified in ISP-2 medium. The 16S rRNA gene sequences were obtained by PCR amplification and sequencing. Through phylogenetic tree and morphology analysis, it was preliminarily identified that the strain may be Kibdelosporangium phytohabitans KLBMP 1111T. The antibacterial activity of fermentation extracts was assayed by the disk susceptibility test and micro-dilution test. The extracts showed a broad-spectrum anti-Gram-positive bacteria effect. Preliminary separation found that there were multiple active ingredients in the crude extract. Maipomycin A was identified in the crude extracts by liquid chromatography-tandem high-resolution mass spectrometry (LC-HRMS), and it showed antibacterial activity against Staphylococcus epidermidis, Listeria monocytogenes and Vibrio parahemolyticus. These findings provide a research basis for the exploitation of mangrove microbial resources to discover new drug leads for pharmaceutical industry as well as new antibacterial or antiseptic substances for the food industry.

Key words： mangrove microbes; rare actinomycete; Kibdelosporangium; antibacterial

Cite this article

ZHANG Junliang , ZHANG Shiling , WU Jiahui , XU Shanshan , LIANG Jinyou , XU Ying . Identification and antimicrobial activity of the rare Actinomyces sp.from mangrove sediment[J]. Food and Fermentation Industries, 2021 , 47(8) : 101 -107 . DOI: 10.13995/j.cnki.11-1802/ts.025382

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