Food and Fermentation Industries >

2021 , Vol. 47 >Issue 8: 224 - 229

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026155

Determination of sugar and sugar alcohol in plant tissues by gas chromatography-tandem mass spectrometry

  • JIAN Naidan ,
  • LI Wenli ,
  • ZHANG Zhuli ,
  • ZHU Xia ,
  • XIAN Xuehai ,
  • NIU Yining
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  • 1(Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou 730070, China)
    2(Linxia Agricultural Product Quality and Safety Center, Linxia 731100, China)

Online published: 2021-05-20

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Abstract

To enhance the detection sensitivity of plant endogenous sugars and sugar alcohols, the quantitative analysis of multi-components by gas chromatography-tandem mass spectrometry (GC-MS/MS) for the determination of glucose, galactose, inositol, mannitol, sorbitol and sucrose was established by using adonitol as an internal standard substance. The sample was extracted by organic solvent and then mixed with pyridine, hydroxylamine hydrochloride and acetic anhydride for derivatization. The gas chromatography separation was performed on an HP-5MS capillary column. And the acetylated sugar and sugar alcohols were determined by MS in multiple reaction monitoring(MRM)mode. The results showed that, in the acquisition mode of mass spectrometry, there was a good linear relationship between the concentration of 6 acetylated sugars and sugar alcohols and detection response which with a correlation coefficients(R2) greater than 0.997. The detection limits (LODs) and quantification(LOQs) were 0.013-0.12 mg/L and 0.045-0.37 mg/L, respectively. The sample recovery rates ranged from 95.38％-102.6％, and the relative standard deviations were 1.28％-3.42％.The method has the advantages of low sample dosage, high accuracy, good precision, and thus could be successfully applied to the analysis of sugars and sugar alcohols in plant tissues at a low concentration.

Key words： sugar and sugar alcohol; derivatization; acetic anhydride; gas chromatography-tandem mass spectrometry; multiple reaction monitoring

Cite this article

JIAN Naidan , LI Wenli , ZHANG Zhuli , ZHU Xia , XIAN Xuehai , NIU Yining . Determination of sugar and sugar alcohol in plant tissues by gas chromatography-tandem mass spectrometry[J]. Food and Fermentation Industries, 2021 , 47(8) : 224 -229 . DOI: 10.13995/j.cnki.11-1802/ts.026155

References

[1] LAM P.Effects of consuming dietary fructose versus glucose on de novo lipogenesis in overweight and obese human subjects[J].Berkeley Scientific Journal, 2011, 15(2):1-4.
[2] GACHON C M M, LANGLOIS-MEURINNE M, SAINDRENAN P.Plant secondary metabolism glycosyltransferases:The emerging functional analysis[J].Trends in Plant Science, 2005, 10(11):542-549.
[3] 尹恒, 王文霞, 赵小明, 等.植物糖生物学研究进展[J].植物学报, 2010(5):521-529.
YIN H, WANG W X, ZHAO X M,et al.Research progress in plant glycobiology[J].Chinese Bulletin of Botany, 2010(5):521-529.
[4] 姜晓满, 田卫, 张海霞, 等.糖类物质的色谱分析[J].药物分析杂志, 2006, 26(8):1 181-1 186.
JIANG X M, TIAN W, ZHANG H X, et al.Determination of sugars by chromatography methods[J].Chinese Journal of Pharmaceutical Analysis, 2006,26(8):1 181-1 186.
[5] 于璐, 周光明, 沈洁, 等.离子交换色谱法测定10种热带水果中的葡萄糖、蔗糖和果糖[J].食品工业科技, 2016(37):94-98.
YU L, ZHOU G M, SHENG J, et al.Determination of glucose,sucrose and fructose in 10 kinds of tropical fruits by ion chromatography[J].Sience and Technology of Food Industry, 2016(37):94-98.
[6] 顾菲菲, 李佳, 杨晨东, 等.碱提灵芝多糖的分离纯化、结构表征及免疫活性评价[J].中草药, 2018(10):2 359-2 364.
GU F F, LI J, YANG C D, et al.Fractionation, structural characterization, and immunomodulatory activity of polysaccharide isolated from Ganoderma lucidum[J].Chinese Traditional and Herbal Drugs, 2018(10):2 359-2 364.
[7] 郑文静, 孙静, 张英, 等.毛细管气相色谱法测定山茱萸多糖的单糖组成[J].现代盐化工, 2018, 45(1):15-16.
ZHENG W J, SUN J, ZHANG Y, et al.Determination of monosaccharides of C.officinalis polysaccharide by capillary chromatography[J].Modern Salt and Chemical Industry, 2018, 45(1):15-16.
[8] 王川丕, 诸力, 刘新, 等.超高效液相色-谱质谱联用技术测定茶叶中8种单、寡糖的含量[J].食品科学, 2014,35(20):164-169.
WANG C P, ZHU L, LIU X, et al.Determination of eight saccharides in teas by ultra performance liquid chromatography-tandem mass spectrometry[J].Food Science, 2014,35(20):164-169.
[9] 吉鑫, 樊双喜, 杨彤晖,等.核磁共振法测定食品中水苏糖的含量[J].食品与发酵工业, 2019, 45(19):251-257.
JI X, FANG S X, YANG T H, et al.Quantitative determination of stachyose in foods by NMR[J].Food and Fermentation Industries, 2019, 45(19):251-257.
[10] 李波, 芦菲, 田素玉.气相色谱法同时测定多糖中的中性糖、糖醛酸、氨基糖和唾液酸[J].食品与发酵工业, 2011, 37(9):208-211.
LI B, LU F, TIAN S Y.Simultaneous determination of neutral sugar,uronic acid,amino sugar and sialic acid of polysaccharides by gas chromatography[J].Food and Fermentation Industries, 2011, 37(9):208-211.
[11] 肖文, 姜红石.MS/MS的原理和GC/MS/MS在环境分析中的应用[J].环境科学与技术, 2004, 27(5):26-28.
XIAO W, JIANG H S.Principle of MS/MS and application of GC/MS/MS to environmental analysis[J].Environmental Science and Technology, 2004, 27(5):26-28.
[12] 林常青. 质谱在多糖结构分析中的应用[J].分析测试技术与仪器, 2005, 11(3):221-227.
LIN C Q.The application of mass spectrum on amylose structure analysis[J].Analysis and Testing Technology and Instruments, 2005, 11(3):221-227.
[13] 丁洁, 闫光玲, 杨培, 等.金银花多糖的指纹图谱及体外抗病毒活性研究[J].中国药房,2020,31(9):1 061-1 067.
DING J, YAN G L, YANG P, et al.Study on fingerprint and in vitro antiviral activity of Lonicera japonica polysaccharide[J].China Pharmacy, 2020,31(9):1 061-1 067.
[14] BOLDIZAR I, FUZFAI Z, MOLNAR-PERL I.Characterization of the endogenous enzymatic hydrolyses of Petroselinum crispum glycosides:Determined by chromatography upon their sugar and flavonoid products[J].Journal of Chromatography A, 2013, 1 293:100-106.
[15] 项萍, 唐喆.气相色谱-质谱联用法测定植物组织中糖与糖醇[J].质谱学报, 2018, 39(3):360-365.
XIANG P, TANG Z.Determination of sugars and sugar alcohols in plant tissues by GC/MS[J].Journal of Chinese Mass Spectrometry Society, 2018, 39(3):360-365.
[16] 李小定, 荣建华, 吴谋成, 等.灰树花多糖粗品及各级分中单糖组成的气相色谱法测定[J].食品科学, 2003(4):110-113.
LI X D, RONG J H, WU M C, et al.Composition analysis in crude polysaccharide and polysaccharide fractions from grifola frondosa by gas chromatography[J].Food Science, 2003(4):110-113.
[17] HOOPER C M, STEVENS T J, SAUKKONEN A, et al.Multiple marker abundance profiling:Combining selected reaction monitoring and data-dependent acquisition for rapid estimation of organelle abundance in subcellular samples[J].Plant Journal, 2017, 104(4):1 345-1 352.
[18] 周洪斌, 熊治渝, 余杨, 等.醋酸酐衍生-气相色谱-质谱联用测定葡萄酒中的糖醇[J].色谱, 2013, 31(8):786-790.
ZHONG H B, XIONG Z Y, YU Y, et al.Determination of alditols in wine by gas chromatography-mass spectrometry after acetate derivatization[J].Chinese Journal of Chromatography, 2013, 31(8):786-790.
[19] KALIORA A C, DEDOUSSIS G V Z, SCHMIDT H, et al.Dietary antioxidants in preventing atherogenesis[J].Atherosclerosis, 2006, 187(1):1-17.
[20] 高庆超, 常应九, 马蓉,等.黑果枸杞酵素发酵前后主要成分分析及其体外抗氧化活性研究[J].食品与发酵工业, 2020, 46(5):275-283.
GAO Q C, CHANG Y J, MA R, et al.Analysis of main components and antioxidant activity in vitro for Lycium ruthenicum Murr.Jiaosu before and after fermentation[J].Food and Fermentation Industries, 2020, 46(5):275-283.
[21] 常秀莲, 王长海, 冯咏梅,等.4种芦荟的物理性质及多糖含量的测定[J].食品与发酵工业, 2003, 29(9):1-5.
CHANG X L, WANG C H, FENG Y M, et al.Research on physical properties and determination of polysaccharide content in aloe[J].Food and Fermentation Industries, 2003, 29(9):1-5.
[22] 胡磊, 郭蓓, 陆海, 等.植物组织中糖与糖醇乙酰化及毛细管气相色谱分析[J].植物学通报, 2004, 21(6):689-699.
HU L,GUO P,LU H, et al.Gas chromatographic analysis of sugar and sugar alcohol derivatives through 1-methylimidazole catalyzed acetylation[J].Chinese Bulletin of Botany, 2004, 21(6):689-699.
[23] 廖洪梅, 戴玲, 魏晓飞, 等.单糖乙酰化条件的优化[J].期刊论文, 2008(5):441-443.
LIAO H M, DAI L, WEI X F, et al.Optimization of conditions for acetylation of monosaccharides[J].Physical Testing and Chemical Analysis(Part B:Chemical Analysis), 2008(5):441-443.
[24] 陈焕文, 胡斌, 张燮, 等.复杂样品质谱分析技术的原理与应用[J].分析化学, 2010, 38(8):1 069-1 088.
CHEN H W, HU B, ZHANG X, et al.Principle and application of ambient mass spectrometry for direct analysis of complex samples[J].Chinese Journal of Analytical Chemistry, 2010, 38(8):1069-1088.
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