Food and Fermentation Industries >

2021 , Vol. 47 >Issue 9: 32 - 41

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026734

Research on the contents and difference of the main secondary metabolites in different kumquat fruits

  • GUO Pengmei ,
  • QIN Yan ,
  • ZHAO Xijuan ,
  • JIAO Bining
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  • 1(Citrus Research Institute,Southwest University & Chinese Academy of Agricultural Sciences,Chongqing 400712,China)
    2(Risk Evaluation Laboratory for Quality & Safety of Citrus Products (Chongqing),Ministry of Agriculture and Rural Affairs, Chongqing 400712,China)
    3(Supervision & Inspection & Testing Center for Quality of Citrus Fruits & Nursery Trees,Ministry of Agriculture and Rural Affairs,Chongqing 400712,China)
    4(College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,China)

Received date: 2021-01-13

  Revised date: 2021-02-23

  Online published: 2021-06-03

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Abstract

Sixty-six secondary metabolites in six kumquat fruits were analyzed by a UPLC-QqQ-MS/MS method.Through statistical analysis and cluster analysis,the difference of thirty-one flavonoids,eight phenolic acids,three limonoids,five alkaloids and nineteen coumarins in kumquat fruits were analyzed from multiple perspectives.The results showed that the contents of flavones were the highest among flavonoids in six kumquat fruits,followed by flavanones.The main phenolic acids of Ningbo round kumquat (Fortunella japonica Swingle),Wenzhou nagami kumquat (Fortunella margarita (Lour.) Swingle),Rongan meiwa kumquat (Fortunella crassifolia Swingle) and Changshou kumquat (Fortunella obovata Tanaka) were sinapic acid.Ferulic acid and chlorogenic acid were the main phenolic acids in Jindou kumquat (Fortunella hindsii Swingle) and Jinganzazhong (Fortunella obovata Tanaka),respectively.N-methyltyramine was the main alkaloid in Jinganzazhong,while tyramine was the main alkaloid in the other kumquats.Scoparone,imperatorin,isomeranzin,meranzin hydrate and auraptene were detected in six kumquats.The results of cluster analysis showed that the secondary metabolism patterns of Ningbo round kumquat and Rongan meiwa kumquat were similar,while the difference among Wenzhou nagami kumquat,Changshou kumquat,Jindou kumquat and Jinganzazhong was relatively large.This study is currently the largest study on the number of secondary metabolites detected by the UPLC-QqQ-MS/MS method.The results also provide data support and scientific basis for the application of kumquats in food,medicine and other fields.

Key words： kumquat; flavonoid; alkaloid; limonoid; coumarin; phenolic acid; UPLC-QqQ-MS/MS

Cite this article

GUO Pengmei , QIN Yan , ZHAO Xijuan , JIAO Bining . Research on the contents and difference of the main secondary metabolites in different kumquat fruits[J]. Food and Fermentation Industries, 2021 , 47(9) : 32 -41 . DOI: 10.13995/j.cnki.11-1802/ts.026734

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