Gene regulation of lager yeast (Saccharomyces pastorianus) against high gravity wort in the early stage of high gravity brewing is crucial for the fermentation process, but the response mechanism is still unclear. In this study, the typical lager yeast M14 was selected as the research object, and the cells of M14 strain were obtained after treatment with normal gravity wort and high gravity wort. The total RNA was extracted for transcriptomic sequencing analysis, and the intracellular metabolites were extracted for metabolomic detection and analysis. The fermentation indexes and sugar utilization under two conditions were also investigated. Transcriptomic analysis showed that 191 genes were significantly regulated, which were identified mainly existed in carbon metabolism and amino acid metabolism pathways through KEGG enrichment and GO enrichment analysis. Metabolomic analysis revealed significant differences for 30 metabolites, and KEGG enrichment analysis indicated that differential metabolites mainly existed in amino acid metabolism. 13 related genes were identified through further analysis. The molecular response mechanism of lager yeast to high gravity wort was explored, which laid a foundation for further revealing the brewing mechanism of high gravity beer and the breeding of high gravity tolerant yeast.
WU Zhuofan
,
HU Zixuan
,
WANG Jinjing
,
LIU Chunfeng
,
NIU Chengtuo
,
ZHENG Feiyun
,
LI Qi
. Multi-omics analysis reveals the mechanism of Saccharomyces pastorianus responding to high gravity wort[J]. Food and Fermentation Industries, 2021
, 47(9)
: 76
-83
.
DOI: 10.13995/j.cnki.11-1802/ts.026049
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