Food and Fermentation Industries >

2021 , Vol. 47 >Issue 10: 58 - 65

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025766

The molecular mechanism of Saccharomyces cerevisiae in response to formic acid stress based on RNA-seq technology

  • ZENG Lingjie ,
  • HUANG Jinxiang ,
  • FENG Pixue ,
  • AN Jiaxing ,
  • SI Zaiyong ,
  • LONG Xiufeng ,
  • YI Yi
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  • 1(College of Biological and Chemical Engineering, Guangxi University of Science and Technology,Liuzhou 545006, China)
    2(Guangxi Key Laboratory of Green Processing of Sugar Resources, Liuzhou 545006, China)

Received date: 2020-09-29

  Revised date: 2020-11-20

  Online published: 2021-06-17

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Abstract

Formic acid, a small molecule acid, is a representative cell inhibitor in lignocellulose hydrolysate, which can inhibit cell growth during the alcohol fermentation of lignocellulose. The differentially expressed genes (DEGs) of Saccharomyces cerevisiae before and after 1.8 g/L formic acid treatment were analyzed by RNA-seq analysis. The results indicated that under formic acid stress, a total of 1 504 DEGs were identified, of which 797 were up-regulated genes and 707 were down-regulated genes. The enrichment analysis of differential genes through GO and KEGG found that these DEGs mainly involved in the pathways including ribosome pathway, meiosis-yeast, MAPK signaling pathway, glycolysis/gluconeogenesis pathway, starch and sucrose metabolism pathway, cysteine and methionine metabolism pathway, and purine metabolism pathway. This study preliminarily revealed the molecular response mechanism of S. cerevisiae under formic acid stress, and can provide a theoretical basis for further research on methods to improve the tolerance of cell inhibitors in lignocellulose hydrolysate.

Key words： formic acid; Saccharomyces cerevisiae; RNA-Seq; molecular mechanism

Cite this article

ZENG Lingjie , HUANG Jinxiang , FENG Pixue , AN Jiaxing , SI Zaiyong , LONG Xiufeng , YI Yi . The molecular mechanism of Saccharomyces cerevisiae in response to formic acid stress based on RNA-seq technology[J]. Food and Fermentation Industries, 2021 , 47(10) : 58 -65 . DOI: 10.13995/j.cnki.11-1802/ts.025766

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