Food and Fermentation Industries >

2021 , Vol. 47 >Issue 11: 68 - 74

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026022

Effect of natto freeze-dried powder on alkaline phosphatase and inflammation in type 2 diabetes

  • WANG Yucong ,
  • XIE Zhixin ,
  • ZHANG Xueyan ,
  • WANG Yingnan ,
  • HAN Jianchun
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  • 1(College of Food Science,Northeast Agricultural University,Harbin 150030,China)
    2(Heilongjiang Green Food Science Research Institute,Harbin 150028,China)
    3(Northeast Agricultural University Hospital,Harbin 150030,China)

Received date: 2020-11-02

  Revised date: 2020-11-21

  Online published: 2021-07-16

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Abstract

To investigate the effects of natto lyophilized powder on alkaline phosphatase, bacterial endotoxin and intestinal inflammatory factors in type 2 diabetes mellitus (T2DM), the type 2 diabetic model was established by intraperitoneal injection of high sugar diet and streptozotocin. The changes of body weight and blood glucose, the activity of intestinal alkaline phosphatase (IAP) in feces and the concentration of bacterial endotoxin were measured, and the concentrations of inflammatory factors (interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interferon gamma (IFN-γ), interleukin-1 (IL-1β), interleukin-4 (IL-4), interleukin-5 (IL-5), interleukin-10 (IL-10), and interleukin-13 (IL-13) growth regulatory oncogene α (KC/GRO)) in the intestine were analyzed by MSD multifactor method. The result showed that compared with the model control group, the fasting blood glucose concentration significantly decreased (P<0.05), the IAP activity increased significantly (P<0.05), the concentration of bacterial endotoxin decreased significantly (P<0.05), and the concentrations of proinflammatory cytokines IL-6, TNF-α, IFN-γ, and IL-1β in the intestinal fluid were significantly reduced (P<0.05), while the concentrations of cytokines IL-4, IL-5, IL-10, and IL-13 increased significantly (P<0.05), and the concentration of chemokine KC/GRO decreased significantly (P<0.05) in the natto lyophilized powder dose group. Therefore, natto lyophilized powder can promote the activity of intestinal alkaline phosphatase, and effectively regulate the blood glucose and inflammatory response in T2DM mice.

Key words： inflammation; diabetes; natto freeze-dried powder; cytokine; intestinal alkaline phosphatase

Cite this article

WANG Yucong , XIE Zhixin , ZHANG Xueyan , WANG Yingnan , HAN Jianchun . Effect of natto freeze-dried powder on alkaline phosphatase and inflammation in type 2 diabetes[J]. Food and Fermentation Industries, 2021 , 47(11) : 68 -74 . DOI: 10.13995/j.cnki.11-1802/ts.026022

References

[1] SHOELSON S E,LEE J,GOLDFINE A B.Inflammation and insulin resistance[J].The Journal of Clinical Investigation,2006,116(7):1 793-1 801.
[2] 李筠.内毒素在肝衰竭形成中的作用及中医药治疗[J].传染病信息,2010,23(5):260-262.
LI J.Effect of endotoxin on the development of liver failure and TCM therapy for endotoxemia[J].Infectious Disease Information,2010,23(5):260-262.
[3] BILSKI J,MAZUR-BIALY A,WOJCIK D,et al.The role of intestinal alkaline phosphatase in inflammatory disorders of gastrointestinal tract[J].Mediators of Inflammation,2017,2017:9 074 601.
[4] BATES J M,AKERLUND J,MITTGE E,et al.Intestinal alkaline phosphatase detoxifies lipopolysaccharide and prevents inflammation in zebrafish in response to the gut microbiota[J].Cell Host Microbe,2007,2(6):371-382.
[5] JANG J Y,KIM T S,CAI J,et al.Nattokinase improves blood flow by inhibiting platelet aggregation and thrombus formation[J].Laboratory Animal Science,2013,29(4):221-225.
[6] 王天琦. 纳豆激酶体内药效和毒性研究[D].沈阳:辽宁大学,2018.
WANG T Q.Study on pharmacodynamics and toxicity of nattokinase in vivo[D].Shenyang:Liaoning University,2018.
[7] IWAI K,NAKAYA N,KAWASAKI Y,et al.Antioxidative functions of natto,a kind of fermented soybeans:Effect on LDL oxidation and lipid metabolism in cholesterol-fed rats[J].Journal of Agricultural Food Chemistry,2002,50(12):3 597-3 601.
[8] 安秀峰,刘河汝,代晓曼,等.纳豆脂肽对人乳腺癌细胞MCF-7的作用[J].食品工业科技,2014,35(3):343-345;350.
AN X F,LIU H R,DAI X M,et al.Effect of natto lipopeptide on human breast cancer MCF-7 cells[J].Science and Technology of Food Industry,2014,35(3):343-345;350.
[9] 晏永球,戴关海,江石平,等.两种发酵食品对糖尿病模型小鼠血糖及血脂的影响[J].中国应用生理学杂志,2018,34(2):130-133.
YAN Y Q,DAI G H,JIANG S P,et al.Effect of two kinds of fermented food on blood glucose and lipids in induced diabetic mice[J].Chinese Journal of Applied Physiology,2018,34(2):130-133.
[10] 牛雅杰.紫锥菊提取物抗胰岛素抵抗作用及功能饮料的研制[D].杨凌:西北农林科技大学,2017.
NIU Y J.Anti-insulin resistance effect of echinacea extract and development of functional drink[D].Yangling:Northwest A&F University,2017.
[11] 金祖汉,王香英,毛培江,等.治疗糖尿病高频中药的降血糖作用研究[J].中国现代应用药学,2009,26(4):267-270.
JIN Z H,WANG X Y,MAO P J,et al.Studies on hypoglycemic effect of high frequency chinese medicines for diabetes[J].Chinese Journal of Modern Applied Pharmacy,2009,26(4):267-270.
[12] 李璐, 王永香,王秀海,等.地龙及其复方治疗糖尿病肾病的机制研究进展[J].中国实验方剂学杂志,2017,23(7):227-234.
LI L,WANG Y X,WANG X H,et al.Research progress on mechanism of pheretima and compound on diabetic nephropathy[J].Chinese Journal of Experimental Traditional Medical Formulae,2017,23(7):227-234.
[13] SUN H L,SUN L,LI Y Y,et al.ACE-inhibitor suppresses the apoptosis induced by endoplasmic reticulum stress in renal tubular in experimental diabetic rats[J].Experimental and Clinical Endocrinology&Diabetes,2009,117(7):336-344.
[14] 舒玲. 银杏叶和丹参提取物与纳豆粉组方的辅助降血脂功能研究[D].大连:大连理工大学,2017.
SHU L.Study on the auxiliary hypolipidemic function of Ginkgo biloba,Salvia miltiorrhiza extract and natto powder[D].Dalian:Dalian University of Technology,2017.
[15] 晏成倩, 黄梅梅,王红平,等.一种难溶性原料药细菌内毒素检查方法的建立[J].中国药师,2018,21(2):365-368.
YAN C Q,HUANG M M,WANG H P,et al.Establishment of bacterial endotoxin test for an insoluble drug substance[J].China Pharmacist,2018,21(2):365-368.
[16] TIGHE P,NEGM O,TODD I,et al.Utility,reliability and reproducibility of immunoassay multiplex kits[J].Methods(San Diego,Calif.),2013,61(1):23-29.
[17] INOOKA S,UEHARA S,KIMURA M.The effect of bacillus natto on the T and B lymphocytes from spleens of feeding chickens[J].Poultry Science,1986,65(6):1 217-1 219.
[18] FUJITA M,HONG K,ITO Y,et al.Thrombolytic effect of nattokinase on a chemically induced thrombosis model in rat[J].Biol Pharm Bull,1995,18(10):1 387-1 391.
[19] 朱运平,李秀婷,李里特.天然α-葡萄糖苷酶抑制剂来源及应用研究现状[J].中国食品学报,2011,11(4):154-159.
ZHU Y P,LI X T,LI L T.The source of the natural α-glucosidase inhibitor and its appliction research situation[J].Journal of Chinese Institute of Food Science and Technology,2011,11(4):154-160.
[20] REED M J,MESZAROS K,ENTES L J.et al.A new rat model of type 2 diabetes:The fat fed,streptozotocin treated rat[J].Metabolism Clinical Experimental,2000,49(11):1 390-1 394.
[21] ZHOU L,HU Y H,LI C Y,et al.Levo corydalmine alleviates vincristine induced neuropathic pain in mice by inhibiting an NF-kappa B dependent CXCL1/CXCR2 signaling pathway[J].Neuropharmacology,2018,135:34-47.
[22] ABBAS A,ASTER J,KUMAR V.Robbins basic pathology[M].9th ed.Amsterdam:Elseviser,2012.
[23] LALLÉS L P.Intestinal alkaline phosphatase:Novel functions and protective effects[J].Journal of Nutrition Reviews,2014(2):82-94.
[24] LU Y C,YEH W C,OHASHI P S.LPS/TLR4 signal transduction pathway[J].Journal of Cytokine,2008,42(2):145-151.
[25] KLAAS P,WINSTON W B,PIETER A K,et al.Dephosphorylation of endotoxin by alkaline phosphatase in vivo[J].American Journal of Pathology,1997,151(4):1 163-1 169.
[26] LALLÈS J P.Intestinal alkaline phosphatase:Multiple biological roles in maintenance of intestinal homeostasis and modulation by diet[J].Nutrition Reviews,2010,68(6):323-332.
[27] 贺云,杨丽霞,邱连利.从肠道微生态角度探讨2型糖尿病的发病机制以及治疗措施[J].中国实验方剂学杂志,2020,26(15):229-234.
HE Y,YANG L X,QIU L L.Discussion on pathogenesis and treatment of type 2 diabetes from perspective of intestinal microecology[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(15):229-234.
[28] 曹靖文,黄占旺,吴少福,等.纳豆菌糖肽对小鼠腹腔巨噬细胞炎症因子分泌的影响[J].营养学报,2015,37(5):509-511.
CAO J W,HUANG Z W,WU S F,et al.Effect of Bacillus natto glycopeptide on inflammatory cytokines secretion of mouse peritoneal macrophages[J].Acta Nutrimenta Sinica,2015,37(5):509-511.
[29] 吴全峰,赵立波,王晓玲,等.炎症性肠病的治疗药物研究进展[J].中国药师,2020,23(10):2 011-2 019.
WU Q F,ZHAO L B,WANG X L,et al.Research progress in therapeutic drugs for inflammatory bowel disease[J].China Pharmacist,2020,23(10):2 011-2 019.
[30] WYNN T A,CHAWLA A,POLLARD J W.Macrophage biology in development,homeostasis and disease[J].Nature,2013,496(7 446):445-455.
[31] 沈柱英,黄占旺,曹靖文.纳豆菌糖肽对免疫功能低下小鼠的免疫调节作用[J].营养学报,2014,36(4):361-365.
SHEN Z Y,HUANG Z W,CAO J W.The immunoregulating effect of bacillus natto glycopeptides in immuno-suppressed mice[J].Acta Nutrimenta Sinica,2014,36(4):361-365.
[32] 尚沁沁,黄琴,徐歆,等.纳豆芽孢杆菌B4对Caco-2细胞活性、黏附与细胞因子分泌的影响[J].动物营养学报,2014,26(1):279-286.
SHANG Q Q,HUANG Q,XU X,et al.Effects of bacillus subtilis var.natto B4 on activity,adhesion and cytokine secretion of caco-2 cells[J].Chinese Journal of Animal Nutrition,2014,26(1):279-286.
[33] 江振洲,袁子航,孙丽新,等.中国药物作用靶点研究进展(Ⅰ)[J].药学进展,2020,44(6):433-450.
JIANG Z Z,YUAN Z H,SUN L X,et al.Progress in research on drug targets in china(Ⅰ)[J].Progress in Pharmaceutical Sciences,2020,44(6):433-450.
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