The enzymatic hydrolysate of collagen was extracted from yak skin by hot water extraction coupled with enzyme hydrolysis. Four kinds of collagen antifreeze peptides with different molecular weights were separated by ultrafiltration membrane technique (<1 k, 1-3 k, 3-5 k and >5 kDa). On this basis, differential scanning calorimetry was used to measure the thermal hysteresis activity of collagen antifreeze peptides. Meanwhile, the cryogenic microscope was used to observe the recrystallization inhibition ability and the characteristics of modified ice crystal growth morphology. The results showed that collagen antifreeze peptides of different molecular weights had the ability to regulate ice growth and thermal hysteresis activity. Among them, the 3-5 kDa collagen antifreeze peptide exhibited the highest thermal hysteresis activity ( 5.77 ℃). Collagen antifreeze peptides with <1 kDa and >5 kDa had the best inhibitory activity against recrystallization. Moreover, collagen antifreeze peptides with 3-5 kDa had the strongest ability to inhibit the growth of single ice crystals. The binding of collagen antifreeze peptides and ice surface could induce rapid growth of ice crystals along with six directions. The collagen-antifreeze peptides less than 1 kDa and more than 5kDa resulted in hexagonal star pattern expansion of ice crystals, while collagen antifreeze peptides between 1-3 kDa and 3-5 kDa resulted in dendritic expansion.
LIU Han
,
CAO Hui
,
XU Fei
,
YUAN Min
,
YE Tai
,
YU Jingsong
,
LI Yan
,
YIN Fengqin
,
WU Xiuxiu
,
HAO Liling
. The antifreeze properties and mechanism of collagen antifreeze peptides with different molecular weights[J]. Food and Fermentation Industries, 2021
, 47(11)
: 104
-110
.
DOI: 10.13995/j.cnki.11-1802/ts.025969
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