Food and Fermentation Industries >

2021 , Vol. 47 >Issue 11: 208 - 215

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026327

Extraction, structural identification and biological activity study of flavonoids from bamboo shoot processing residue (BSDF)

  • YANG Bo ,
  • YI Sha ,
  • SHI Kaiyun ,
  • LI Qin ,
  • YANG Guang ,
  • HE Liang
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  • 1(School of Medical Instrument and Food Engineering,University of Shanghai for Science and Technology,Shanghai 200931,China)
    2(Bamboo Shoots Engineering Research Center of the State Forestry Bureau,Zhejiang Forestry Academy, Zhejiang Provincial Key Laboratory of Bamboo Shoot Research,Hangzhou 310231,China)

Received date: 2020-12-03

  Revised date: 2021-01-01

  Online published: 2021-07-16

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Abstract

In this study, flavonoids were extracted from bamboo shoot processing residue (BSDF) using alcohol. The extraction rate of total flavonoids was taken as the response to analyzing the influences of ethanol concentration, solid-liquid ratio and extraction time on the extraction effect. Then, the extraction process of BSDF was optimized by response surface to determine the optimal extraction conditions,which were 55% ethanol, material-to-liquid ratio of 27∶1 (mL∶g) extraction for 66 min and the optimal extraction rate was 0.33%. The results of liquid chromatography-mass spectrometry (UPLC-Triple TOF/MS) showed that there were four main types of carbon glycosides in BSDF which were schaftoside, chrysin 6-C-arabinoside 8-C-glucoside, apigenin-6,8-C-arabinoside, chrysin 6-C-β-D-glucoside-8-C-α-L-arabinoside. Moreover, in vitro biological activity of BSDF showed that low, medium and high concentrations of BSDF had inhibitory effects on human hepatoma (HepG2), which were enhanced with the increase of BSDF concentration. It was proved that BSDF had certain anti-tumor activity. Meanwhile, the model of HepG2 cells insulin resistance (HepG2-IR) was established to detect the hypoglycemic activity of BSDF extracts in vitro. Results showed that when BSDF extract on HepG2-IR cells (15 μg/mL insulin-induced) in 400 μg/mL, the glucose consumption were (3.51±0.28) mmol/L. It was greater than insulin treatment group and less than metformin treatment group. This indicates that BSDF extract can improve the glucose consumption of HepG2-IR cells.

Key words： bamboo shoots processing residue; bamboo shoots roots; flavonoids; antitumor activity; hypoglycemic activity

Cite this article

YANG Bo , YI Sha , SHI Kaiyun , LI Qin , YANG Guang , HE Liang . Extraction, structural identification and biological activity study of flavonoids from bamboo shoot processing residue (BSDF)[J]. Food and Fermentation Industries, 2021 , 47(11) : 208 -215 . DOI: 10.13995/j.cnki.11-1802/ts.026327

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