Food and Fermentation Industries >

2021 , Vol. 47 >Issue 11: 259 - 265

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026351

Determination of nine flavonoids in celery seed by liquid chromatography-tandem mass spectrometry

  • HUANG Huang ,
  • WANG Fengling ,
  • ZHAO Wei ,
  • WANG Xueqing ,
  • ZHANG Tingting
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  • (Tianjin Key Laboratory of Food Biotechnology,College of Biotechnology and Food Science, Tianjin University of Commerce,Tianjin 300134,China)

Received date: 2020-12-02

  Revised date: 2021-01-21

  Online published: 2021-07-16

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Abstract

A laboratory method for the detection of the concentration of nine flavonoids (apigenin, rutin, baicalin, baicalein, luteolin, quercetin, wogonin, apigenin, coylidin) in celery seeds were established based on liquid chromatography-tandem mass spectrometry. The relevant parameters such as sample extraction, purification, liquid phase separation and tandem mass spectrometry were optimized. The sample was extracted with a material-to-liquid ratio of 1∶20 (g∶mL), 70% volume fraction of ethanol aqueous solution by ultrasonic for 60 min, and purified by AB-8 macroporous resin. Using acetonitrile-0.1% formic acid water solution as mobile phase, the sample was separated by ZORBAX Eclipse XDB-C18 chromatographic column and detected by electrospray negative ions, MRM mode. The results showed that the nine flavonoids had a good linear relationship in the mass concentration range of 0.5-20.0 μg/mL, the correlation coefficient r2≥0.999 0, and the recovery rates of the nine flavonoids were all 88.0%-106.2% (relative standard deviation was 0.7%-4.9%), the detection limits of nine flavonoids were 10-66 μg/kg. This method is simple, and the results is accurate and reliable and with good stability. It is suitable for the simultaneous detection of nine flavonoids in celery seeds.

Key words： celery seed; flavonoids; macroporous resin purification; liquid chromatography-tandem mass spectrometry (LC-MS/MS)

Cite this article

HUANG Huang , WANG Fengling , ZHAO Wei , WANG Xueqing , ZHANG Tingting . Determination of nine flavonoids in celery seed by liquid chromatography-tandem mass spectrometry[J]. Food and Fermentation Industries, 2021 , 47(11) : 259 -265 . DOI: 10.13995/j.cnki.11-1802/ts.026351

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