Food and Fermentation Industries >

2021 , Vol. 47 >Issue 12: 83 - 89

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026920

In vitro antioxidant activities and inhibitory activities of α-glucosidase and acetylcholinesterase in Rubus parvifolius L. root extract

  • LIN Zilan ,
  • SHA Xiaomei ,
  • ZHANG Zhibin ,
  • XIA Qile ,
  • SUN Rui ,
  • WANG Zhenxing
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  • 1(College of Forestry, Southwest Forestry University, Kunming 650224, China)
    2(College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China)
    3(Zhejiang Academy of Agricultural Sciences, Food Science Institute, Hangzhou 310021, China)
    4(Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China,Ministry of Education,
    Southwest Forestry University, Kunming 650224, China)
    5(College of Life Sciences, Southwest Forestry University, Kunming 650224, China)

Received date: 2021-01-29

  Revised date: 2021-02-16

  Online published: 2021-07-22

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Abstract

To further investigate the function of Rubus parvifolius L. Root, an ultrasound-assisted methanol extraction assay was used to prepare Rubus parvifolius L. extract (RRE). The total phenolic content (TPC) and total flavonoid content (TFC) were determined, and the in vitro antioxidant activity was also assessed by measuring DPPH, ABTS, and superoxide anions radical scavenging abilities, as well as ferric reducing antioxidant power. In addition, the α-glucosidase and acetylcholinesterase inhibition activity of RRE were evaluated using fluorescence spectroscopy and UV-VIS spectrometry. The results showed that the TPC and TFC of RRE were 446.392 mg GAE/g and 638.337 mg Rutin/g. RRE had the antioxidant activities with values of 871.878 mg Trolox/g, 1 177.854 mg Trolox/g, 419.928 mg Trolox/g, and 1 827.378 mg FeSO4/g for DPPH, ABTS, superoxide anions radical scavenging abilities, and ferric reducing antioxidant power, respectively, and also inhibited α-glucosidase and acetylcholinesterase with the IC50 values of 1.314 mg/mL and 403.347 μg/mL. Taken together, RRE had certain in vitro antioxidant, α-glucosidase and acetylcholinesterase inhibition capacities, and could be developed as natural antioxidants, hypoglycemic and anti-dementia drugs. This research can provide scientific evidence for the development and utilization of Rubus parvifolius L.

Key words： Rubus parvifolius L.Root; extract; in vitro antioxidation; α-glucosidase inhibition; acetylcholinesterase inhibition

Cite this article

LIN Zilan , SHA Xiaomei , ZHANG Zhibin , XIA Qile , SUN Rui , WANG Zhenxing . In vitro antioxidant activities and inhibitory activities of α-glucosidase and acetylcholinesterase in Rubus parvifolius L. root extract[J]. Food and Fermentation Industries, 2021 , 47(12) : 83 -89 . DOI: 10.13995/j.cnki.11-1802/ts.026920

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