The immunomodulatory activity of Lactobacillus plantarum WHH589 with extracellular polysaccharide-producing property and its application in fermented milk

  • SUN Sheng ,
  • CHEN Zuoguo ,
  • YU Yunxia ,
  • QU Dongmei ,
  • YU Tengfei ,
  • LI Yanjun ,
  • CHEN Su
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  • (Key Laboratory of Food and Biological Engineering of Zhejiang Province,Hangzhou Wahaha Technology Co.Ltd.,Hangzhou Wahaha Group Co.Ltd.,Hangzhou 310018,China)

Received date: 2021-01-08

  Revised date: 2021-01-28

  Online published: 2021-08-02

Abstract

Lactic acid bacteria (LAB) were isolated from Chinese traditional fermented foods to screen the specific strains with high exopolysaccharide yields. The basic characteristics, the immunomodulatory ability and the application in fermented milk were further explored of these LAB. Colony wire drawing test as well as sulfuric acid-phenol method had been utilized to screen the LAB with high exopolysaccharide production, and the effect of LAB on proliferation of mouse spleen lymphocytes were also determined through MTT assay. The results showed that Lactobacillus plantarum WHH589, which separated from Sichuan pickles, exhibited the strongest ability to produce exopolysaccharide. The yield reached 0.58 g/L. The results of MTT assay suggested that L. plantarum WHH589 and exopolysaccharide could significantly promote the proliferation of mouse spleen up to 79.79%-127.55% and 80.15%-164.42%, respectively. which lead to beneficial immunomodulatory effects. In the meanwhile, L. plantarum WHH589 exhibited good growth characteristics, acid, bile salt resistance and intestinal adhesion ability. Moreover, the yogurt fermented by L. plantarum WHH589 possessed a solid texture and obvious drawing with the length reached 25.57 cm. The sensory and flavor were good. The viable count reached 4.97×108 CFU/g. In summary, L. plantarum WHH589 showed great development and utilization value because of fermentation and probiotic properties.

Cite this article

SUN Sheng , CHEN Zuoguo , YU Yunxia , QU Dongmei , YU Tengfei , LI Yanjun , CHEN Su . The immunomodulatory activity of Lactobacillus plantarum WHH589 with extracellular polysaccharide-producing property and its application in fermented milk[J]. Food and Fermentation Industries, 2021 , 47(13) : 43 -50 . DOI: 10.13995/j.cnki.11-1802/ts.026564

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