Food and Fermentation Industries >

2021 , Vol. 47 >Issue 13: 98 - 106

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026722

Breeding of Saccharomyces cerevisiae strains with appropriate yield of acetate esters

  • WEI Yanan ,
  • WANG Jinxiao ,
  • LIN Liangcai ,
  • CHEN Bochao ,
  • CUI Danyao ,
  • ZHANG Cuiying
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  • 1(Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,Tianjin University of Science and Technology,Tianjin 300457,China)
    2(College of Bioengineering,Tianjin University of Science and Technology,Tianjin 300457,China)

Received date: 2021-01-15

  Revised date: 2021-02-20

  Online published: 2021-08-02

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Abstract

Ethyl acetate and isoamyl acetate are two important volatile flavor substances in Chinese Baijiu. Low concentration of ester could not give Baijiu the characteristic taste, while the flavor of Baijiu was also damaged by hyper-production of ester. In previous work, ATF1 overexpression with BAT2 deletion in Saccharomyces cerevisiae a45 (a45-AWT) enhanced the content of acetate esters and decreased the content of higher alcohols. However, the concentration of isoamyl acetate was quite high, thereby causing negative effects on the quality of Baijiu. In this work, isoamyl acetate hydrolyzing esterase coding gene IAH1 was deleted to evaluate the effect on the content of acetate esters and higher alcohols in Baijiu. The strain a45-AWT was used as the parental strain. IAH1 was overexpressed and a45-BAI strain was obtained. Fermentation analysis showed that the isoamyl acetate production of a45-BAI was only 10.73 mg/L, which was decreased by 83.2%. The proportion of ethyl acetate and isoamyl acetate of strain a45-BAI was increased by 87.3%. In this study, The strain with high yield of ethyl acetate was constructed by regulating the of ester biosynthesis and biolysis. It could moderate production of isoamyl acetate and low production of higher alcohols. The results provided new strategies for strain breeding.

Key words： Saccharomyces cerevisiae; isoamyl acetate hydrolysing esterase; ethyl acetate; isoamyl acetate; concentrated fermentation; Chinese Baijiu

Cite this article

WEI Yanan , WANG Jinxiao , LIN Liangcai , CHEN Bochao , CUI Danyao , ZHANG Cuiying . Breeding of Saccharomyces cerevisiae strains with appropriate yield of acetate esters[J]. Food and Fermentation Industries, 2021 , 47(13) : 98 -106 . DOI: 10.13995/j.cnki.11-1802/ts.026722

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