To substitute nitrite with fresh roses in semi-dried fermented sausage processing, rose extract was prepared by sugar extraction, and response surface methodology was used to optimize the procedure of rose extraction. Sensory scores, total flavonoids content, and color indexes were used as indicators, and single-factor experiments were performed on the ratio of water to flowers, the ratio of sugar to flowers, citric acid addition amount, and the extraction time. On this basis, the response surface design of Box-Behnken was carried out, and the regression equation was constructed to obtain the optimal extraction parameters. The rose extract prepared under the optimal process conditions was added to the fermented sausage to replace partial nitrite. The color difference, nitrous residue and sensory quality characteristics of fermented sausages were studied through the combination of different added amounts of nitrite (40, 60, 80, 100 mg/kg) and different contents of rose extract (6%, 10% and 14%). The optimal combination of sodium nitrite and rose extract was determined. The final results showed that the rose solution was bright red in color, moderate in acidity and sweetness, and rich in total flavonoids under the conditions of water flowers ratio of 0.7∶1 (mL∶g), sugar flowers ratio of 1.5∶1 (g∶g), citric acid 0.3% (mass fraction), and the extraction time for 1.75 h. When 10% rose extract and 80 mg/kg sodium nitrite were added to the fermented sausage, the quality was significantly better than that of the fermented sausage with 150 mg/kg sodium nitrite. It is feasible to partially replace the nitrite in semi-dried fermented sausage with rose extract.
[1] 龙强, 聂乾忠,刘成国.发酵香肠研究进展及展望[J].食品科学,2017,38(13):291-298.
LONG Q,NIE Q Z,LIU C G.Fermented sausage:Recent progress and prospects[J].Food Science,2017,38(13):291-298.
[2] HAMMAD H H M,MEIHU M,ELGASIM A E,et al.Nitroso-hemoglobin-ginger conjugates effects on bacterial growth and color stability in a minced beef model[J].International Journal of Food Microbiology,2020,331.DOI:10.1016/j.ijfoodmicro.2020.108731.
[3] MICHAUD D S,HOLICK C N,BATCHELOR T T,et al.Prospective study of meat intake and dietary nitrates,nitrites,and nitrosamines and risk of adult glioma[J].American Journal of Clinical Nutrition,2009,90(3):570-577.
[4] NAMRYE L,CHEONGSIK K,GEONSEONG Y,et al.Effect of nitrite substitution of sausage with addition of purple sweet potato powder and purple sweet potato pigment[J].Journal of the Korean Society of Food Science and Nutrition,2015,44(6):896-903.
[5] 朱培培, 唐宏刚,肖朝耿,等.甜菜粉与亚硝酸钠复合对中式香肠品质的影响[J].食品工业科技,2017,38(1):269-272;327.
ZHU P P,TANG H G,XIAO C G,et al.Effects of beet powder combined with sodium nitrite on the quality of Chinese sausage[J].Science and Technology of Food Industry,2017,38(1):269-272;327.
[6] 郑淑彦, 王伟,董金金,等.食用玫瑰营养保健功能及产品开发研究进展[J].食品研究与开发,2016,37(23):206-211.
ZHEN S Y,WANG W,DONG J J,et al.Research progress on nutrition health function and product development of edible rose[J].Food Research and Development,2016,37(23):206-211.
[7] SHIKOV V,KAMMERER D R,MIHALEV K,et al.Heat stability of strawberry anthocyanins in model solutions containing natural copigments extracted from rose(Rosa damascena Mill.) petals[J].Journal of Agricultural and Food Chemistry,2008,56(18):8 521-8 526.
[8] 高春燕, 刘颖,李文鹏.玫瑰花色素微波辅助提取及其对亚硝酸盐清除作用的研究[J].食品工业科技,2008,29(4):207-209.
GAO C Y,LIU Y,LI W P.Study on extraction of rose pigment by microwave and its scavenging capability to nitrite[J].Science and Technology of Food Industry,2008,29(4):207-209.
[9] 冯作山, 谢琼,余永婷,等.玫瑰花渣中抗氧化成分提取工艺研究[J].食品工业科技,2007,28(8):143-145.
FENG Z S,XIE Q,YU Y T,et al.Study on extraction technology of antioxidant components from rose residue[J].Science and Technology of Food Industry,2007,28(8):143-145.
[10] 孙迪,付本宁,张志国.玫瑰花生物活性物质的研究进展[J].食品工业,2016,37(7):243-245.
SUN D,FU B N,ZHANG Z G.Research progress of roses bioactive substances[J].Food Industry,2016,37(7):243-245.
[11] 刘万臣,刘爱萍,赵榕,等.肉制品加工及贮存过程中亚硝酸盐含量的变化与安全性分析[J].食品科学,2010,31(1):113-116.
LIU W C,LIU A P,ZHAO R,et al.Change and safety assessment of nitrite in meat products during processing and storage[J].Food Science,2010,31(1):113-116.
[12] DEWANTO V,WU X Z,ADOM K K,et al.Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity[J].Journal of Agricultural and Food Chemistry,2002,50(10):3 010-3 014.
[13] 张冰晶, 蒋玉梅,米兰,等.发酵型苦水玫瑰花酱的制作工艺参数优化[J].中国酿造,2014,33(6):147-152.
ZHANG B J,JIANG Y M,MI L,et al.Brewing technology of rosa rugosa jam[J].China Brewing,2014,33(6):147-152.
[14] 李美娟, 陈国宏,陈衍泰.综合评价中指标标准化方法研究[J].中国管理科学,2004,12(增刊1):45-48.
LI M J,CHEN G H,CHEN Y T.Research on index standardization method in comprehensive evaluation[J].Chinese Journal of Management Science,2004,12(Sup.1):45-48.
[15] 黄金枝, 杨荣玲,刘学铭,等.发酵广式香肠菌种配比优化研究[J].食品科技,2014,39(11):143-146.
HUANG J Z,YANG R L,LIU X M,et al.Optimize of strain ratio on cantonese fermented sausage[J].Food Science and Technology,2014,39(11):143-146.
[16] 王慧英. 玫瑰红色素的理化性质、生物活性及提取应用研究进展[J].食品与机械,2018,34(11):174-177.
WANG H Y.Research advances on property,extractionand application of redpigment from rose[J].Food & Machinery,2018,34(11):174-177.
[17] 郑华, 张弘,张忠和.天然动植物色素的特性及其提取技术概况[J].林业科学研究,2003,16(5):628-635.
ZHEN H,ZHANG H,ZHANG Z H.Characteristics and extraction technology of natural pigments from animals and plants[J].Forest Research,2003,16(5):628-635.
[18] 陆庆. 桃花黄酮提取纯化、稳定性与抗氧化性研究及其结构鉴定[D].南昌:江西农业大学,2015.
LU Q.Extraction,purification,stability,antioxidation and structural identification of flavonoids from prunus persica flos[D].Nanchang:Jiangxi Agricultural University,2015.
[19] 张志国, 姜闪.食用玫瑰花褪色原因及控制措施研究进展[J].食品科学,2017,38(9):322-328.
ZHANG Z G,JIANG S.Causes and control measures of the fading of edible roses[J].Food Science,2017,38(9):322-328.
[20] ELS V,MARIANA U,STEFAAN D S,et al.Dog rose(Rosa canina L.) as a functional ingredient in porcine frankfurters without added sodium ascorbate and sodium nitrite[J].Meat Science,2012,92(4):451-457.
[21] LARA M S,GUTIERREZ J I,TIMÓN M,et al.Evaluation of two natural extracts(Rosmarinus officinalis L.and Melissa officinalis L.) as antioxidants in cooked pork patties packed in MAP[J].Meat Science,2011,88(3):481-488.