Proteinase-producing Pseudomonas is the main harmful bacteria in raw milk. The aim of this study was to establish a feasible duplex PCR method for the rapid detection of proteinase-producing Pseudomonas. Two pairs of specific primers were designed according to the sequences of sucD gene and aprX gene in Pseudomonas. The primers were evaluated by their specificity, sensitivity and detection limits. The results demonstrated that this method had good specificity and the detection limit was 0.167 pg/μL for pure DNA. Meanwhile, after 12 h and 24 h enrichment cultivation, the detection limits of this method in artificially contaminated raw milk were 3.5×103 CFU/mL and 3.5 CFU/mL, respectively. In conclusion, this PCR method could be widely used for the rapid detection of proteinase-producing Pseudomonas in raw milk.
WU Tianci
,
LI Nan
,
ZHANG Juan
,
YU Yi
,
LIU Zhenmin
. Development and evaluation of a duplex PCR method for the detection of proteinase-producing Pseudomonas in raw milk[J]. Food and Fermentation Industries, 2021
, 47(14)
: 251
-256
.
DOI: 10.13995/j.cnki.11-1802/ts.026652
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