Effects of fortifying patterns on the characteristics of strong flavor type Daqu

  • CHEN Xiaoru ,
  • HUANG Jun ,
  • ZHOU Rongqing ,
  • ZHANG Suyi ,
  • DONG Yi ,
  • WANG Chao ,
  • WANG Xiaojun ,
  • WU Chongde ,
  • JIN Yao
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  • 1(College of Biomass Science and Engineering,Sichuan University,Chengdu 610065,China)
    2(Sichuan LUZHOULAOJIAO Co.Ltd.,Luzhou 646000,China)

Received date: 2020-11-16

  Revised date: 2020-12-12

  Online published: 2021-08-23

Abstract

Similarities and differences of metabolic components and microbial communities in strong-flavor type Daqu that fortified by isolate culture and high quality Daqu powder, which were referred as C and S samples, were investigated by polyphasic examination approaches. The results indicated that there were significant differences of the microbial community diversity, physicochemical properties and volatiles between C and S samples. When fortified by isolate, the abundance of bacteria and fungi increased. The diversity of fungi increased, while bacteria and physicochemical indexes decreased. The content of pyrazines and alcohols were enhanced by 5 074.49% and 440.50%, respectively. Nevertheless, the richness and diversity of bacteria increased, while the fungi decreased in S samples. Besides, the contents of major volatiles, including esters, alcohols, acids and pyrazines were increased significantly. The esterification ability was enhanced by 34.41%, meanwhile, some unique components were only detected in S sample, such as caryophyllene (209.06±15.07) μg/kg and ledol (183.88±15.07) μg/kg. The results laid the foundation for the bioturbation effect of fortified Daqu on microbial communities and metabolic profiles.

Cite this article

CHEN Xiaoru , HUANG Jun , ZHOU Rongqing , ZHANG Suyi , DONG Yi , WANG Chao , WANG Xiaojun , WU Chongde , JIN Yao . Effects of fortifying patterns on the characteristics of strong flavor type Daqu[J]. Food and Fermentation Industries, 2021 , 47(15) : 50 -55 . DOI: 10.13995/j.cnki.11-1802/ts.026190

References

[1] ZHENG X W,HAN B Z.Baijiu (白酒),Chinese liquor:History,classification and manufacture[J].Journal of Ethnic Foods,2016,3(1):19-25.
[2] 王小平, 黄永光,周文美.酱香型白酒大曲细菌菌群结构及功能研究进展[J].中国酿造,2020,39(2):19-25.
WANG X P,HUANG Y G,ZHOU W M.Research progress on structure and function of bacterial community in Moutai-flavor Baijiu Daqu[J].China Brewing,2020,39(2):19-25.
[3] DU H,WANG X S,ZHANG Y H,et al.Exploring the impacts of raw materials and environments on the microbiota in Chinese Daqu starter[J].International Journal of Food Microbiology,2019,297:32-40.
[4] LI H,HUANG J,LIU X,et al.Characterization of interphase microbial community in Luzhou-flavored liquor manufacturing pits of various ages by polyphasic detection methods[J].Journal of Microbiol and Biotechnol,2016,27(1):130-140.
[5] LI P,LIN W F,LIU X,et al.Effect of bioaugmented inoculation on microbiota dynamics during solid-state fermentation of Daqu starter using autochthonous of Bacillus,Pediococcus,Wickerhamomyces and Saccharomycopsis[J].Food Microbiology,2017,61:83-92.
[6] 崔香香, 白飞荣,于学健,等.谢瓦散囊菌CICC 41584产香特性及在浓香型白酒大曲生产中的应用[J].食品与发酵工业,2019,45(21):60-67.
CUI X X,BAI F Y,YU X J,et al.Aroma characteristics of Eurotium chevalieri CICC 41584 and its application in aroma Baijiu Daqu production[J].Food and Fermentation Industries,2019,45(21):60-67.
[7] 冯利芳, 李素琴,刘鹏烩,等.清香大曲中性蛋白酶产生菌的分离鉴定及应用研究[J].食品科技,2018,43(11):1-6.
FENG L F,LI S Q,LIU P H,et al.Isolation,identification and application of bacteria producing neutral protease form light-flavor Daqu[J].Food Science and Technology,2018,43(11):1-6.
[8] Li W,FAN G,FU Z,et al.Effects of fortification of Daqu with various yeasts on microbial community structure and flavor metabolism[J].Food Research International,2019,129:108 837.
[9] 吴秋霞, 黄钧,江东材,等.基于共培强化提高浓香大曲吡嗪类及4-乙基愈创木酚组分含量的研究[J].食品科技,2017,42(5):2-6.
WU Q X,HUANG J,JIANG D C,at al.Exploring the improvement of contents pf pyrazines and 4-EG in Luzhou-flavor Daqu based on fortifying by co-culture[J].Food Science and Technology,2017,42(5):2-6.
[10] 敖宗华, 陕小虎,沈才洪,等.国内主要大曲相关标准及研究进展[J].酿酒科技,2010(2):104-108.
AO Z H,SHAN X H,SHEN C H,at al.Domenstic related quality standards of Daqu & its research progress[J].Liquor-Making Science & Technology,2010(2):104-108.
[11] 中华人民共和国工业和信息化部. QB/T 4 257-2011 酿酒大曲通用分析方法[S].北京:食品发酵标准化中心,2012.
Ministry industry and information Technology of the People’s Republic of China.QB/T 4 257-2011 General methods of analysis for Daqu[S].Beijing:Standardization Center for Food Fermentation,2012.
[12] DING X F,WU C D,HUANG J,et al.Characterization of interphase volatile compounds in Chinese Luzhou-flavor liquor fermentation cellar analyzed by head space-solid phase micro extraction coupled with gas chromatography mass spectrometry (HS-SPME/GC/MS)[J].LWT-Food Science and Technology,2016,66:124-133.
[13] CAPORASO J G,KUCZYNSKI J,STOMBAUGH J,et al.QIIME allows analysis of high-throughput community sequencing data[J].Nature Methods,2010,7:335-336.
[14] EDGAR R C.Search and clustering orders of magnitude faster than BLAST[J].Bioinformatics,2010,26(19):2 460-2 461.
[15] 刘婷, 陈可丹,黄冰静,等.不同生产环境对特香型大曲的影响[J].中国酿造,2019,38(7):80-84.
LIU T,CHEN K D,HUANG B J,et al.Effect of different production environment on Te-flavour Daqu[J].China Brewing,2019,38(7):80-84.
[16] WANG X S,DU H,ZHANG Y,et al.Environmental microbiota drives microbial succession and metabolic profiles during Chinese liquor fermentation[J].Applied and Environmental Microbiology,2018,84(4):e2 369-2 317.
[17] 束莹. 古井贡酒大曲中微生物群落结构的鉴定及分析[D].哈尔滨:哈尔滨工业大学,2013.
SU Y.Identification and analysis of Microbial community structure for Gujing liquor Daqu[D].Harbin:Harbin Institute of Technology,2013.
[18] YAN S B,TONG Q Q,GUANG J Q.Yeast dynamics and changes in volatile compounds during the fermentation of the traditional Chinese strong-flavor Daqu[J].LWT,2019,106:57-63.
[19] 夏屿. 浓香型大曲主发酵阶段微生物群落结构变化及与挥发性化合物的相关性研究[D].自贡:四川轻化工大学,2019.
XIA Y.Correlationship between microbiota and volatile compounds in Luzhou-flavour Daqu of the main fermentation stages[D].Zigong:Sichuan University of Science & Engineering.
[20] 鲜文东, 明红,李文均.高温放线菌科最新研究进展小型综述[J].微生物学报,2015,55(1):1-11.
XIAN W D,MING H,LI W J.The family Thermoactinomycetaceae-A review[J].Journal of Microbiology,2015,55(1):1-11.
[21] WANG P,WU Q,JIANG X,et al.Bacillus licheniformis affects the microbial community and metabolic profile in the spontaneous fermentation of Daqu starter for Chinese liquor making[J].International Journal of Food Microbiology,2017,250:59-67.
[22] 梁晨, 杜海,徐岩.大曲贮存过程中原核微生物群落结构及风味成分演替规律[J].微生物学通报,2017,44(2):384-393.
LIANG C,DU H,XU Y.The succession of procaryotic microbial community and the flavor components in storage process of Daqu[J].Microbiology China,2017,44(2):384-393.
[23] 刘从艾, 穆文斌,汤有宏.大曲糖化力、发酵力最佳量比关系的研究[J].酿酒,2000,27(2):41-43.
LIU C A,MU W B,TANG Y H.Study on optimum quantity ratio of Saccharification power and fermentation power of Daqu[J].Liquor Making,2000,27(2):41-43.
[24] 唐玉明, 沈才洪,任道群,等.酒曲理化品质指标相关性探讨[J].酿酒科技,2006(7):37-41.
TANG Y M,SHENG C H,REN D Q,et al.Investigation on the correlations of physiochemical indexes of starter[J].Liquor-Making Science & Technology,2006(7):37-41.
[25] 范文来, 徐岩.白酒79个风味化合物嗅觉阈值测定[J].酿酒,2011,38(4):80-84.
FAN W L,XU Y.Determination of odor thresholds of volatile aroma compounds in Baijiu by a forced-choice ascending concentration series method of limits[J].Liquor Making,2011,38(4):80-84.
[26] 易琍娟. 白酒中α-联酮类物质的作用及其来源之探讨[J].中国酿造,1983,2(4):14-19.
YI L J.Study on the effects of α-diketones in liquor and their sources[J].China Brewing,1983,2(4):14-19.
[27] WANG H J,QI H Y,ZHANG J S.Analysis of volatile components in black pepper essential oil[J].China Condiment,2017,42(12):138-140;146.
[28] 王超, 张连忠,彭福田.顶空固相微萃取法分析肥桃香气成分[C].西安:中国园艺学会桃分会第三届学术研讨会论文集,2011.
WANG C,ZHANG L Z,PENG F T.Headspace solid phase microextraction was used to analyze the aroma components of fatty peach[C].Xi’an:Proceedings of the Third Symposium of the Peach Branch of the Chinese Horticultural Society,2011.
[29] ZHANG R,WU Q,XU Y.Aroma characteristics of Moutai-flavour liquor produced with Bacillus licheniformis by solid-state fermentation[J].Letters in Applied Microbiology,2013,57(1):11-18.
[30] 龙可, 赵中开,马莹莹,等.酿酒根霉菌研究进展[J].现代食品科技,2013,29(2):443-447.
LONG K,ZHAO Z K,MA Y Y,et al.Progresses of researches on rhizopus for liquor-making[J].Modern Food Science and Technology,2013,29(2):443-447.
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