Food and Fermentation Industries >

2021 , Vol. 47 >Issue 15: 77 - 83

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026448

Potentials of Lactobacillus strains as oral probiotics candidates

  • YU Yi ,
  • WANG Chaoyue ,
  • WU Zhengjun ,
  • ZHANG Jia ,
  • WU Tianci
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  • 1(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China)
    2(Research Institute,Bright Dairy and Food Co.Ltd.,Shanghai 200436,China)

Received date: 2020-12-21

  Revised date: 2020-12-30

  Online published: 2021-08-23

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Abstract

Four Lactobacillus strains were evaluated in vitro by demonstrate high hydrophobicity, electrostatic charge, tolerance to lysozyme and lower biofilm formation ability. The auto-aggregation ability of four tested Lacticaseibacillus rhamnosus B6, Limosilactobacillus fermentum B44, Lacticaseibacillus casei LC2W and Lactiplantiacillus plantarum ST-III were 37.67%-60.89%, while their co-aggregating with Streptococcus mutans, the predominant pathogen causing dental caries in oral cavity, reached 63.47%, 46.42%, 60.5% and 61.51%, respectively. When cocultured with S. mutans in BHI containing 3 g/L sucrose, they could reduce the formation of S. mutans biofilm by 31.04%-54.28%, which might be attributed to synergetic effect of organic acids as well as other antagonistic substances produced by Lactobacilli. In conclusion, four tested strains have potentials as oral probiotics candidates in preventing dental caries.

Key words： Lactobacillus; Streptococcus mutans; dental caries; biofilm; exopolysaccharides

Cite this article

YU Yi , WANG Chaoyue , WU Zhengjun , ZHANG Jia , WU Tianci . Potentials of Lactobacillus strains as oral probiotics candidates[J]. Food and Fermentation Industries, 2021 , 47(15) : 77 -83 . DOI: 10.13995/j.cnki.11-1802/ts.026448

References

[1] AYTAC BAL F,OZKOCAK I,CADIRCI B H,et al.Effects of photodynamic therapy with indocyanine green on Streptococcus mutans biofilm[J].Photodiagnosis and Photodynamic Therapy,2019,26:229-234.
[2] KASSEBAUM N J,BERNABÉ E,DAHIYA M,et al.Global burden of untreated caries:A systematic review and metaregression[J].Journal of Dental Research,2015,94(5):650-658.
[3] DU M Q,LI Z,JIANG H,et al.Dental caries status and its associated factors among 3-to 5-year-old children in china:A national survey[J].Chinese Journal of Dental Research,2018,21(3):167-179.
[4] XIAO J,KLEIN M I,FALSETTA M L,et al.The exopolysaccharide matrix modulates the interaction between 3D architecture and virulence of a mixed-species oral biofilm[J].PLoS Pathogens,2012,8(4):e1002623.
[5] YUE J X,YANG H Y,LIU S Y,et al.Influence of naringenin on the biofilm formation of Streptococcus mutans[J].Journal of Dentistry,2018,76:24-31.
[6] HE J Z,WANG S D,WU T X,et al.Effects of ginkgoneolic acid on the growth,acidogenicity,adherence,and biofilm of Streptococcus mutans in vitro[J].Folia Microbiologica,2013,58(2):147-153.
[7] KULSHRESTHA S,KHAN S,HASAN S,et al.Calcium fluoride nanoparticles induced suppression of Streptococcus mutans biofilm:An in vitro and in vivo approach[J].Applied Microbiology and Biotechnology,2016,100(4):1 901-1 914.
[8] KOO H,DUARTE S,MURATA R M,et al.Influence of cranberry proanthocyanidins on formation of biofilms by Streptococcus mutans on saliva-coated apatitic surface and on dental caries development in vivo[J].Caries Research,2010,44(2):116-126.
[9] KIM D,HWANG G,LIU Y,et al.Cranberry flavonoids modulate cariogenic properties of mixed-species biofilm through exopolysaccharides-matrix disruption[J].PLoS One,2015,10(12).DOI:10.1371/journal.pone.0145844.
[10] ANDRÉ C B,ROSALEN P L,GALVÃO L C D C,et al.Modulation of Streptococcus mutans virulence by dental adhesives containing anti-caries agents[J].Dental Materials,2017,33(10):1 084-1 092.
[11] NATTO Z S,HAMEEDALDAIN A.Methodological quality assessment of meta-analyses and systematic reviews of the relationship between periodontal and systemic diseases[J].Journal of Evidence Based Dental Practice,2019,19(2):131-139.
[12] JAKUBOVICS N S.Oral microbiology.Encyclopedia of Microbiology (Fourth Edition)[M].Oxford:Academic Press.2019.
[13] TEANPAISAN R,PIWAT S,TIANVIWAT S,et al.Effect of long-term consumption of Lactobacillus paracasei SD1 on reducing mutans streptococci and caries risk:A randomized placebo-controlled trial[J].Dentistry Journal,2015,3(2):43-54.
[14] SIDHU G K,MANTHA S,MURTHI S,et al.Evaluation of Lactobacillus and Streptococcus mutans by addition of probiotics in the form of curd in the diet[J].Journal of International Oral Health,2015,7(7):85-89.
[15] FERNANDES T,BHAVSAR C,SAWARKAR S,et al.Current and novel approaches for control of dental biofilm[J].International Journal of Pharmaceutics,2018,536(1):199-210.
[16] COMPARE D,ROCCO A,COCCOLI P,et al.Lactobacillus casei DG and its postbiotic reduce the inflammatory mucosal response:An ex-vivo organ culture model of post-infectious irritable bowel syndrome[J].BMC Gastroenterology,2017,17(1):53
[17] 姚沛琳. 乳酸菌抑制变异链球菌生物膜形成的研究[D].无锡:江南大学,2015.
YAO P L.Inhibition of lactic acid bacteria on the biofilm formation of Streptococcus mutans[D].Wuxi:Jiangnan University,2015.
[18] ZHANG G,LU M,LIU R M,et al.Inhibition of Streptococcus mutans biofilm formation and virulence by Lactobacillus plantarum K41 isolated from traditional Sichuan pickles[J].Frontiers in Microbiology,2020,11:774.
[19] WU C C,LIN C T,WU C Y,et al.Inhibitory effect of Lactobacillus salivarius on Streptococcus mutans biofilm formation[J].Molecular Oral Microbiology,2015,30(1):16-26.
[20] SAMOT J,LEBRETON J,BADET C.Adherence capacities of oral Lactobacilli for potential probiotic purposes[J].Anaerobe,2011,17(2):69-72.
[21] KHAN R,ZAKIR M,KHANAM Z,et al.Novel compound from Trachyspermum ammi (Ajowan caraway) seeds with antibiofilm and antiadherence activities against Streptococcus mutans:A potential chemotherapeutic agent against dental caries[J].Journal of Applied Microbiology,2010,109(6):2 151-2 159.
[22] WASFI R,MANSOUR L E,HASHEM A M,et al.Antimicrobial activities against biofilm formed by Proteus mirabilis isolates from wound and urinary tract infections[J].Indian Journal of Medical Microbiology,2012,30(1):76-80.
[23] RIBEIRO S C,O’CONNOR P M,ROSS R P,et al.An anti-listerial Lactococcus lactis strain isolated from Azorean Pico cheese produces lacticin 481[J].International Dairy Journal,2016,63:18-28.
[24] MATSUMOTO-NAKANO M,NAGAYAMA K,KITAGORI H,et al.Inhibitory effects of Oenothera biennis (evening primrose) seed extract on Streptococcus mutans and S.mutans-induced dental caries in rats[J].Caries Research,2011,45(1):56-63.
[25] 徐显睿, 李翠凤,杨洪来,等.具有益生潜力的乳酸杆菌筛选[J].乳业科学与技术,2020,43(2):8-12.
XU X R,LI C F,YANG H L,et al.Screening of Lactobacillus for probiotic potential[J].Journal of Dairy Science and Technology,2020,43(2):8-12.
[26] PERES C M,ALVES M,HERNANDEZ-MENDOZA A,et al.Novel isolates of Lactobacilli from fermented Portuguese olive as potential probiotics[J].LWT-Food Science and Technology,2014,59(1):234-246.
[27] 薛龙, 王立鹤,聂红兵,等.唾液乳酸杆菌w22a和种植体周围炎致病菌体外作用的初步研究[J].口腔医学研究,2016,32(10):1 042-1 046.
XUE L,WANG L H,NIE H B,et al.Antibacterial effects of Lactobacillus salivarius W22a on the periodontal pathogens[J].Journal of Oral Science Research,2016,32(10):1 042-1 046.
[28] 张秋香, 黄银,姚沛琳,等.植物乳杆菌FB-T9抑制变异链球菌及其生物膜形成的研究[J].食品与生物技术学报,2019,38(9):17-26.
ZHANG Q X,HUANG Y,YAO P L,et al.Inhibition of Streptococcus mutans and its biofilm formation by Lactobacillus plantarum FB-T9[J].Journal of Food Science and Biotechnology,2019,38(9):17-26.
[29] SULAIMAN N D,NIKEN A,NUR I J,et al.Isolation and partial purification of lysozyme from saliva of Bali cattle (Bos sondaicus) using an aqueous mixture of polyethylene glycol (PEG) with sodium sulfate[J].African Journal of Biotechnology,2012,11(8):1 977-1 980.
[30] TORLAKOVIC L,KLEPAC-CERAJ V,ØGAARD B,et al.Microbial community succession on developing lesions on human enamel[J].Journal of Oral Microbiology,2012,4(1):16 125.
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