The rhizome of Amomum villosum was used as raw material to obtain the purified polysaccharide (AVRP-1), and the structural characteristics and antioxidant activities of AVRP-1 were further studied. DEAE-52 and Sephadex G-100 column chromatography were used to obtain AVRP-1. And the structure of AVRP-1 was characterized by chemical composition, high-performance gel permeation chromatography (HPGPC), HPLC and Fourier transform infrared (FT-IR) spectroscopy. The antioxidant activity of AVRP-1 was assessed based on the DPPH and hydroxyl radical scavenging rates in vitro. The results showed that the content of total sugar, protein and uronic acid of AVRP-1 was 90.34%, 0.64% and 43.59%, respectively. The relative molecular weight of AVRP-1 was 3 372.01 kDa. Moreover, AVRP-1 was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose in the molar ratio of 4.14∶4.98∶5.10∶23.77∶17.30∶28.51∶16.19. The glycosidic bonds existed in both and configurations. The assay of antioxidant activity of AVRP-1 revealed that its half-maximal effective concentration (EC50) for DPPH and hydroxyl radical scavenging activity was 4.50 and 2.47 mg/mL, respectively. These results showed that AVRP-1 could be used as a natural antioxidant substance in the pharmaceutical and functional food industry, and provide a theoretical basis for making full use of A. villosum.
[1] 姜春兰, 蔡锦源, 梁莹, 等.砂仁的有效成分及其药理作用的研究进展[J].轻工科技, 2020, 36(7):43-47.
JIANG C L, CAI J Y, LIANG Y, et al.Research progress on active components and pharmacological effects of Amomum villosum[J].Light Industry Science and Technology, 2020, 36(7):43-45;47.
[2] 尤小梅, 李远志, 廖有传, 等.春砂仁根和叶提取物抗氧化活性研究[J].食品科技, 2012, 37(2):226-229.
YOU X M, LI Y Z, LIAO Y C, et al.Antioxidant activity of the extracts from Amomum villosum roots and leaves[J].Food Science and Technology, 2012, 37(2):226-229.
[3] WANG J Q, HU S Z, NIE S P, et al.Reviews on mechanisms of in vitro antioxidant activity of polysaccharides[J].Oxidative Medicine and Cellular Longevity, 2016,64:1-13.
[4] RAMBERG J E, NELSON E D, SINNOTT R A.Immunomodulatory dietary polysaccharides:A systematic review of the literature[J].Nutrition Journal, 2010, 9(1):54-76.
[5] YANG X, JI H Y, FENG Y Y, et al.Structural characterization and antitumor activity of polysaccharides from Kaempferia galanga L.[J].Oxidative Medicine and Cellular Longevity, 2018.DOI:10.1155/2018/9579262.
[6] CUI J F, YE H, ZHU Y J, et al.Characterization and hypoglycemic activity of a rhamnan-type sulfated polysaccharide derivative[J].Marine Drugs, 2019, 17(21):1-14.
[7] ZHANG D Y, LI S J, XIONG Q P, et al.Extraction, characterization and biological activities of polysaccharides from Amomum villosum[J].Carbohydrate Polymers, 2013, 95(1):114-122.
[8] ZHANG J N, XIONG Q P, LI S J, et al.A comparison study on polysaccharides from novel hybrids of Amomum villosum and its female parent[J].International Journal of Biological Macromolecules, 2015, 81:396-399.
[9] YAN Y J, LI X, WAN M J, et al.Effect of extraction methods on property and bioactivity of water-soluble polysaccharides from Amomum villosum[J].Carbohydrate Polymers, 2015, 117(6):632-635.
[10] DUBOIS M, GILLES K A, HAMILTON J K, et al.Colorimetric method for determination of sugars and related substances[J].Analytical Chemistry, 1956, 28:350-356.
[11] BRADFORD M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J].Analytical Biochemistry, 1976, 72(1-2):248-254.
[12] BLUMENKRANTZ N, ASBOE-HANSEN G.New method for quantitative determination of uronic acids[J].Analytical Biochemistry, 1973, 54(2):484-489.
[13] 黄冬. 狗脊糖聚物的分离纯化、结构鉴定及抗骨质疏松活性研究[D].广州:广东药科大学, 2018.
HUANG D.Isolation, purification, structural characterization of saccharides from Cibotium barometz and its anti-osteoporosis activity[D].Guangzhou:Guangdong Pharmaceutical University, 2018.
[14] WANG Y F, JIA J X, REN X J, et al.Extraction, preliminary characterization and in vitro antioxidant activity of polysaccharides from Oudemansiella radicata mushroom[J].International Journal of Biological Macromolecules, 2018, 120:1 760-1 769.
[15] LI C, HUANG Q, FU X, et al.Characterization, antioxidant and immunomodulatory activities of polysaccharides from Prunella vulgaris Linn[J].International Journal of Biological Macromolecules, 2015, 75:298-305.
[16] DU B, ZENG H S, YANG Y D, et al.Anti-inflammatory activity of polysaccharide from Schizophyllum commune as affected by ultrasonication[J].International Journal of Biological Macromolecules, 2016, 91:100-105.
[17] WANG Y, LI X, ZHAO P, et al.Physicochemical characterizations of polysaccharides from Angelica sinensis Radix under different drying methods for various applications[J].International Journal of Biological Macromolecules, 2019, 121:381-389.
[18] DUAN M Y, SHANG H M, CHEN S L, et al.Physicochemical properties and activities of comfrey polysaccharides extracted by different techniques[J].International Journal of Biological Macromolecules, 2018, 115:876-882.
[19] HU Z Y, ZHOU H L, ZHAO J L, et al.Microwave-assisted extraction, characterization and immunomodulatory activity on RAW264.7 cells of polysaccharides from Trichosanthes kirilowii Maxim seeds[J].International Journal of Biological Macromolecules, 2020, 164:2 861-2 872.
[20] GUO R, TIAN S, LI X J, et al.Pectic polysaccharides from purple passion fruit peel:A comprehensive study in macromolecular and conformational characterizations[J].Carbohydrate Polymers, 2020, 229(1):115406.
[21] ZHU D Y, MA Y L, WANG C H, et al.Insights into physicochemical and functional properties of polysaccharides sequentially extracted from onion (Allium cepa L.)[J].International Journal of Biological Macromolecules, 2017, 105:1 192-1 201.
[22] BELTRAME G, TRYGG J, RAHKILA J, et al.Structural investigation of cell wall polysaccharides extracted from wild Finnish mushroom Craterellus tubaeformis (Funnel Chanterelle)[J].Food Chemistry, 2019, 301(15):125255.
[23] CHEN Y Q, LIU D, WANG D Y, et al.Hypoglycemic activity and gut microbiota regulation of a novel polysaccharide from Grifola frondosa in type 2 diabetic mice[J].Food and Chemical Toxicology, 2019, 126:295-302.
[24] FU Y, LI F, DING Y, et al.Polysaccharides from loquat (Eriobotrya japonica) leaves:Impacts of extraction methods on their physicochemical characteristics and biological activities[J].International Journal of Biological Macromolecules, 2020, 146:508-517.
[25] ZHAO C C, LI X, MIAO J, et al.The effect of different extraction techniques on property and bioactivity of polysaccharides from Dioscorea hemsleyi[J].International Journal of Biological Macromolecules, 2017, 102:847-856.
[26] ZHANG L, HU Y, DUAN X Y, et al.Characterization and antioxidant activities of polysaccharides from thirteen boletus mushrooms[J].International Journal of Biological Macromolecules, 2018, 113:1-7.
[27] ZHU Z Y, DONG F Y, LIU X C, et al.Effects of extraction methods on the yield, chemical structure and anti-tumor activity of polysaccharides from Cordyceps gunnii mycelia[J].Carbohydrate Polymers, 2016, 140(20):461-471.
