Food and Fermentation Industries >

2021 , Vol. 47 >Issue 16: 258 - 262

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027120

Determination of 2-methylimidazole and 4-methylimidazole in caramel pigment using GC-PCI MS/MS

  • QIAO Qingqing ,
  • HAO Lihua ,
  • GONG Fan ,
  • GE Jing ,
  • FANG Yuke
Expand
  • 1(Henan Institute of Quality Supervision and Inspection, Zhengzhou 450000, China)
    2(Henan key laboratory of food safety data intelligence, Zhengzhou 450000, China)
    3(Zhongkai University of Agriculture and Engineering, Guangzhou 510000, China)

Received date: 2021-02-25

  Revised date: 2021-04-16

  Online published: 2021-09-10

Fold

Abstract

Gas chromatography-positive chemical ionization-tandem triple quadruple mass spectrometry (GC-PCI MS/MS) for determination of 2-methylimidazole and 4-methylimidazole residue in caramel pigment was established. The samples were first alkalized and purified by kieselguhr. And then they were eluted with ethyl acetate. The detection was carried out by GC-PCI MS/MS. A good linear relationship was obtained in the concentrations of 0.5-8.0 μg/mL, while the correlation coefficient of 2-methylimidazole was 0.999 7 and 4-methylimidazole was 0.999 4 respectively. The detection limit of this method was 20 mg/kg. The recoveries of three spiked levels (20, 80 and 320 mg/kg) were in the range of 102.1%-107.9% with RSDs (n=3) which between 1.33%-5.79%. This method developed here was simple, rapid, sensitive and accurate to meet the needs of the analysis. And it could be used for the detection of 2-methylimidazole and 4-methylimidazole in caramel pigment.

Key words： gas chromatography-positive chemical ionization-tandem triple quadruple mass spectrometry (GC-PCI MS/MS); caramel pigment; 2-methylimidazole; 4-methylimidazole

Cite this article

QIAO Qingqing , HAO Lihua , GONG Fan , GE Jing , FANG Yuke . Determination of 2-methylimidazole and 4-methylimidazole in caramel pigment using GC-PCI MS/MS[J]. Food and Fermentation Industries, 2021 , 47(16) : 258 -262 . DOI: 10.13995/j.cnki.11-1802/ts.027120

References

[1] 熊珺, 陈美玲, 赖毅东.固相萃取-选择离子气质联用法测定液态调味料中的4-甲基咪唑[J].现代食品科技, 2012, 28(5):567-569;578.
XIONG J, CHEN M L, LAI Y D.Determination of 4-methylimidazole in liquid seasoning by solid phase extraction combined with gas chromatography/selected ion mass spectrometry[J].Modern Food Science and Technology, 2012, 28(5):567-569;578.
[2] CUNHA S C, BARRADO A I, FARIA M A, et al.Assessment of 4-(5-) methylimidazole in soft drinks and dark beer[J].Journal of Food Composition and Analysis, 2011, 24(4-5):609-614.
[3] 庞美蓉, 林钦, 戴明, 等.超高效液相色谱-串联质谱法同步检测食品中的2-甲基咪唑及4-甲基咪唑[J].分析测试学报, 2016, 35(8):943-948.
PANG M R, LIN Q, DAI M, et al.Simultaneous determination of 2-methylimidazole and 4-methylimidazole in various foods by ultrahigh performance liquid chromatography-tandem mass spectrometry[J].Journal of Instrumental Analysis, 2016, 35(8):943-948.
[4] 张丽华, 邵瑞婷, 杨丽梅,等.超高效液相色谱串联质谱法测定蚝油中 3种甲基咪唑类物质[J].食品与发酵工业, 2019, 45(24):229-233.
ZHANG L H, SHAO R T, YANG L M, et al.Determination of three methylimidazoles in oyster sauce using ultra-high performance liquid chromatography-tandem mass spectrometry[J].Food and Fermentation Industries, 2019, 45(24):229-233.
[5] 王淼, 陈海珍.高效液相色谱法测定焦糖中4-甲基咪唑的含量[J].中国处方药, 2015, 13(10):25-26.
WANG M, CHEN H Z.Determination of 4-methylimidazole in caramel by high performance liquid chromatography[J].Journal of China Prescription Drug, 2015, 13(10):25-26.
[6] 吴玉銮, 冼燕萍, 郭新东, 等.分散固相萃取-超高效液相色谱-串联质谱法测定酱油中的2-甲基咪唑和4-甲基咪唑[J].食品安全质量检测学报, 2014, 5(5):1 357-1 364.
WU Y L, XIAN Y P, GUO X D, et al.Quantitative determination of 2- and 4-methylimidazole in soy sauce using dispersive solid phase extraction and ultra-high performance liquid chromatography-tandem mass spectrometry[J].Journal of Food Safety & Quality, 2014, 5(5):1 357-1 364.
[7] CHAN P, MAHLER J, TRAVLOS G, et al.Induction of thyroid lesions in 14-week toxicity studies of 2 and 4-methylimidazole in Fischer 344/N rats and B6C3F1 mice[J].Archives of Toxicology, 2006, 80(3):169-180.
[8] CHAN P C, HILLlS G D, KISSLING G E, et al.Toxicity and carcinogenicity studies of 4-methylimidazole in F344/N rats and B6C3F1 mice[J].Archives of Toxicology, 2008, 82(1):45-53.
[9] 陈洪兴, 戴军, 陈尚卫,等.高效液相色谱法测定焦糖色素中的4-甲基咪唑[J].中国调味品, 2006,31(6):53-56.
CHEN H X, DAI J, CHEN S W, et al.Determination of 4 - methylimdiazole in caramel pingments by high performance liquid chromatography[J].China Condiment, 2006,31(6):53-56.
[10] 程利侠, 郝华玲, 吕长平, 等.三种咪唑物质检测技术研究进展[J].湖南文理学院学报 (自然科学版), 2021, 33(1):86-89.
CHENG L X, HAO H L, LYU C P,et al.Recent advances in analytical methods of three imidazole substances[J].Journal of Hunan University of Arts and Science (Science and Technology), 2021, 33(1):86-89.
[11] 孟颖. 焦糖色素着色食品中甲基咪唑测定方法的研究及应用[D].太原:山西大学, 2014.
MENG Y.The research and application of the detection methods of methylimidazoles in Caramel food[D].Taiyuan:Shanxi University, 2014.
[12] 肖义夫, 廖百森.高效液相色谱法测定焦糖色素中4-甲基咪唑[J].现代预防医学, 2005,32(3):249-250.
XIAO Y F, LIAO B S.Determination of 4- Methyl inidazole in caramel by HPLC[J].Modern Preventive Medicine, 2005,32(3):249-250.
[13] 王颖, 黄璐瑶, 余晓琴, 等.高效液相色谱-串联质谱法测定豆制品中3种甲基咪唑类化合物[J].中国卫生检验杂志, 2018, 28(4):415-418.
WANG Y, HUANG L Y, YU X Q, et al.Simultaneous determination of 3 methylimidazoles in bean product by high performance liquid chromatography-tandem mass spectrometry[J].Chinese Journal of Health Laboratory Technology, 2018, 28(4):415-418.
[14] 王爱军. 气相色谱串联三重四级杆质谱测定焦糖色中4-甲基咪唑[J].食品工业, 2019,40(7):321-323.
WANG A J.Determination of 4-methylimidazole in caramel color by capillary gas chromatography-tandem mass spectrometry[J].The Food Industry, 2019,40(7):321-323.
[15] 王冰玉, 赵文英, 曹晓荣, 等.高效液相色谱法测定咪唑生产工艺中反应液中咪唑及2种杂质2-甲基咪唑和4-甲基咪唑[J].理化检验-化学分册, 2020, 56(6):692-695.
WANG B Y, ZHAO W Y, CAO X R, et al.HPLC determination of the imidazole and 2 impurities of 2-methylimidazole and 4-methylimidazole in reaction solutions in production process of imidazole[J].Physical Testing and Chemical Analysis Part B(Chemical Analysis), 2020, 56(6):692-695.
[16] 公丕学, 廉贞霞, 薛霞, 等.固相萃取UPLC-MS/MS测定黄酒中2-甲基咪唑和4-甲基咪唑[J].食品研究与开发, 2019, 40(14):136-142.
GONG P X, LIAN Z X, XUE X, et al.Determination of 2-methylimidazole and 4-methylimidazole in yellow wine by solid phase extraction ultra-high performance liquid chromatography-tandem mass spectrometry[J].Food Research and Development, 2019, 40(14):136-142.
[17] 钮正睿, 赵梅, 刘素丽,等.超高效液相色谱-串联质谱法检测保健食品中4-甲基咪唑含量[J].食品安全质量检测学报, 2019, 10(14):4 613-4 618.
NIU Z R, ZHAO M, LIU S L, et al.Determination of 4-methylimidazole in health food by ultra performance liquid chromatography tandem mass spectrometry[J].Journal of Food Safety & Quality, 2019, 10(14):4 613-4 618.
[18] 林晓珊, 杭义萍, 吴惠勤, 等.气相色谱-串联质谱法测定焦糖色素中2-甲基咪唑和4-甲基咪唑[J].分析试验室, 2011, 30(11):85-88.
LIN X S, HANG Y P, WU H Q, et al.Determination of 2-methylimidazole and 4-methylimidazole in caramel pigment by gas chromatography-tandem mass spectrometry[J].Chinese Journal of Analysis Laboratory, 2011, 30(11):85-88.
[19] 张丽华, 邵瑞婷, 杨丽梅, 等.超高效液相色谱串联质谱法测定蚝油中3种甲基咪唑类物质[J].食品与发酵工业, 2019, 45(24):229-233.
ZHANG L H,SHAO R T,YANG L M, et al.Determination of three methylimidazoles in oyster sauce using ultra-high performance liquid chromatography-tandem mass spectrometry[J].Food and Fermentation Industries, 2019, 45(24):229-233.
[20] 幸苑娜, 王欣, 陈泽勇, 等.气相色谱-正化学源质谱法测定家用橡胶手套中7种N-亚硝胺及其前体物的迁移量[J].分析测试学报, 2011, 30(5):503-508.
XING Y N, WANG X, CHEN Z Y, et al.Determination of migration levels of 7 N-nitrosamines and N-nitrosatable substances from household gloves by chromatography-positive chemical ionization mass spectrometry[J].Journal of Instrumental Analysis, 2011, 30(5):503-508.
Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.