Improvement of low-mass β-glucan yield by adaptive laboratory evolution and its antioxidant activity

  • WANG Bing ,
  • ZHU Li ,
  • LI Maowei ,
  • ZHAN Xiaobei
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  • 1(Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education,School of Biotechnology, Jiangnan University,Wuxi 214122,China)
    2(Wuxi Galaxy Biotech Co.Ltd.,Wuxi 214125,China)

Received date: 2020-12-11

  Revised date: 2021-01-06

  Online published: 2021-09-27

Abstract

β-Glucan is considered as a potential functional polysaccharide. The adaptive laboratory evolution was used to induce Agrobacterium sp. to produce low molecular weight β-glucan with glycerol, and the antioxidant activity was determined. Agrobacterium sp.WS-8E3T produced, a novel polysaccharide, β-gluco-oligosaccharides (BGOs) with 2 800-3 600 Da stably after 120 h in 100 g/L glycerol shake flask. The BGOs was analyzed by infrared spectrum, monosaccharide composition and NMR, which proved that BGOs was β-1,3-glucan. The BGOs had antioxidant capacity with the scavenging rate of DPPH radical and hydroxyl radical reached to 80% of ascorbic acid. The results showed that the BGOs had better antioxidant properties, and provides the basis for the application of BGOs in the functional food and medicine field.

Cite this article

WANG Bing , ZHU Li , LI Maowei , ZHAN Xiaobei . Improvement of low-mass β-glucan yield by adaptive laboratory evolution and its antioxidant activity[J]. Food and Fermentation Industries, 2021 , 47(17) : 27 -33 . DOI: 10.13995/j.cnki.11-1802/ts.026445

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