Bovine α-lactalbumin (BLA) can induce allergic reactions and is related to intestinal microbiota.The effects of BLA modification on allergenicity and human intestinal microbiota were investigated by modifying BLA with different protein modification techniques.In this study,glycationed,phosphorylated and acetylated BLA was used as the object of study.Electrophoresis,spectroscopy and ELISA were used to study the changes of its structure and IgE binding ability.16S rRNA sequencing was used to analyze the structure and composition of human intestinal microbiota.The results showed that the structure of BLA was changed by phosphorylation,glycation and acetylation.After modification,the IgE binding ability of BLA decreased gradually.High-throughput sequencing of 16S rRNA showed that,compared with BLA,the modified BLA decreased the relative abundance of Bacteroidota and increased the relative abundance of Firmicutes at the phylum level.At the genus level,it significantly increased the relative abundance of Megamonas and Romboutsia,but decreased the relative abundance of Prevotella and Faecalibacterium.The results showed that glycation,phosphorylation and acetylation reduced the allergenicity of BLA by destroying its structure and affected the structure of human intestinal microbiota to the extent of acetylated>glycation>phosphorylation
LIU Jun
,
WANG Yang
,
XIONG Zihao
,
CHEN Wenmei
,
SHAO Yanhong
,
TU Zongcai
. Effects of glycation,phosphorylation and acetylation on allergenicity and human intestinal microbiota of α-lactalbumin[J]. Food and Fermentation Industries, 2021
, 47(17)
: 91
-97
.
DOI: 10.13995/j.cnki.11-1802/ts.028011
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