Food and Fermentation Industries >

2021 , Vol. 47 >Issue 17: 126 - 132

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027283

Protective effects of shrimp peptides on hydrogen peroxide-induced oxidative damage in RAW264.7 Cells

  • SHAO Wan ,
  • WANG Rui ,
  • ZHOU Yufang ,
  • XIANG Xingwei ,
  • SUN Jipeng ,
  • WANG Jiaxing ,
  • LIAO Miaofei ,
  • DENG Shanggui ,
  • ZHENG Bin
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  • 1(College of Food and Pharmacy,Zhejiang Ocean University,Zhoushan 316021,China)
    2(Zhejiang Marine Development Institute,Zhoushan 316021,China)
    3(College of Food Science and Technology,Zhejiang University of Technology,Hangzhou 310014,China)

Received date: 2021-03-11

  Revised date: 2021-04-01

  Online published: 2021-09-27

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Abstract

The aim of the study was to investigate the protective effects of shrimp peptides against hydrogen peroxide (H2O2) induced oxidative damage in RAW264.7 cells by CCK-8 assay,enzyme linked immunosorbent assay (ELISA),realtime-PCR and Western Blotting.The results from CCK-8 assay showed that shrimp peptides could significantly increase viability of cells compared with RAW 264.7 cells exposed to H2O2(P<0.05).Moreover,shrimp peptides significantly increase the levels of superoxide dismutase (SOD),glutathione reductase (GSH-Px) and catalase (CAT) in RAW264.7 cells.RT-PCR showed that shrimp peptides could increase the gene expression levels of SOD1,GPX1,Nrf2,NQO1 and HO-1(P<0.05).In addition,shrimp peptides could also observably increase the protein expression level of Nrf2 and decrease the protein expression level of Keap1 with Western Blotting.Collectively,this study suggested that shrimp peptides could effectively protect RAW264.7 cells against H2O2-induced oxidative damage.This protection mechanism may be related to activation of the Nrf2/Keap1 signaling pathway.

Key words： shrimp peptides; hydrogen peroxide; RAW264.7 cells; oxidative damage; protect

Cite this article

SHAO Wan , WANG Rui , ZHOU Yufang , XIANG Xingwei , SUN Jipeng , WANG Jiaxing , LIAO Miaofei , DENG Shanggui , ZHENG Bin . Protective effects of shrimp peptides on hydrogen peroxide-induced oxidative damage in RAW264.7 Cells[J]. Food and Fermentation Industries, 2021 , 47(17) : 126 -132 . DOI: 10.13995/j.cnki.11-1802/ts.027283

References

[1] WOJTOVICH A P,BERRY B J,GALKIN A.Redox signaling through compartmentalization of reactive oxygen species:Implications for health and disease[J].Antioxidants and Redox Signaling,2019,31(9):591-593.
[2] DI MEO S,VENDITTI P.Evolution of the knowledge of free radicals and other oxidants[J].Oxidative Medicine and Cellular Longevity,2020,2020:9 829 176.
[3] LI Y,LI J,LIN S J,et al.Preparation of antioxidant peptide by microwave-assisted hydrolysis of collagen and its protective effect against H2O2-induced damage of RAW 264.7 cells[J].Marine Drugs,2019,17(11):E642.
[4] HU D,SUN X Y,LIAO X D,et al.Alpha-synuclein suppresses mitochondrial protease ClpP to trigger mitochondrial oxidative damage and neurotoxicity[J].Acta Neuropathologica,2019,137(6):939-960.
[5] ROBINSON N,GANESAN R,HEGEDÜS C,et al.Programmed necrotic cell death of macrophages:Focus on pyroptosis,necroptosis,and parthanatos[J].Redox Biology,2019,26:101 239.
[6] MORE G K,MAKOLA R T.In-vitro analysis of free radical scavenging activities and suppression of LPS-induced ROS production in macrophage cells by Solanum sisymbriifolium extracts[J].Scientific Reports,2020,10:6 493.
[7] GROM A A,MELLINS E D.Macrophage activation syndrome:advances towards understanding pathogenesis[J].Current Opinion in Rheumatology,2010,22(5):561-566.
[8] WANG X,MA J,BAI X,et al.Antioxidant properties of astaxanthin produced by cofermentation between Spirulina platensis and recombinant Saccharomyces cerevisiae against mouse macrophage RAW 264.7 damaged by H2O2[J].Food and Bioproducts Processing,2019,118:318-325.
[9] 江杨阳,杨水兵,余海霞,等.不同冻结方式对红虾肌肉品质的影响[J].现代食品科技,2017,33(10):142-148;116.
JIANG Y Y,YANG S B,YU H X,et al.Effects of different freezing methods on the quality of red shrimp[J].Modern Food Science and Technology,2017,33(10):142-148;116.
[10] ZHANG Z W,JIANG S Q,ZENG Y,et al.Antioxidant peptides from Mytilus coruscus on H2O2-induced human umbilical vein endothelial cell stress[J].Food Bioscience,2020,38:100 762.
[11] 邢瀚文,韩玮,施文正,等.固态发酵法制备罗非鱼皮胶原蛋白肽及其抗氧化活性研究[J].食品与发酵工业,2020,46(19):104-110.
XING H W,HAN W,SHI W Z,et al.Tilapia skin collagen peptides prepared by solid-state fermentationand its antioxidant activity[J].Food and Fermentation Industries,2020,46(19):104-110.
[12] WONG F C,XIAO J B,ONG M G L,et al.Identification and characterization of antioxidant peptides from hydrolysate of blue-spotted stingray and their stability against thermal,pH and simulated gastrointestinal digestion treatments[J].Food Chemistry,2019,271:614-622.
[13] LI Y,LI J,LIN S J,et al.Preparation of antioxidant peptide by microwave-assisted hydrolysis of collagen and its protective effect against H2O2-induced damage of RAW 264.7 cells[J].Marine Drugs,2019,17(11):642.
[14] TIAN X X,ZHENG J W,XU B G,et al.Optimization of extraction of bioactive peptides from Monkfish (Lophius litulon) and characterization of their role in H2O2-induced lesion[J].Marine Drugs,2020,18(9):468.
[15] LAN C,ZHAO Y Q,LI X R,et al.High Fischer ratio oligopeptides determination from Antartic krill:Preparation,peptides profiles,and in vitro antioxidant activity[J].Journal of Food Biochemistry,2019,43(5):e12 827.
[16] 韩立华,王菲,戴宇峰,等.南极磷虾肽对去卵巢骨质疏松症小鼠肠钙吸收的改善作用研究[J].中国海洋药物,2017,36(5):42-48.
HAN L H,WANG F,DAI Y F,et al.The improvement of peptide from Antarctic krill on intestinal calcium absorption in ovariectomized mice with osteoporosis[J].Chinese Journal of Marine Drugs,2017,36(5):42-48.
[17] JI W,ZHANG C H,JI H W.Two novel bioactive peptides from Antarctic krill with dual angiotensin converting enzyme and dipeptidyl peptidase IV inhibitory activities[J].Journal of Food Science,2017,82(7-9):1 742-1 749.
[18] JIANG S Q,ZHANG Z W,YU F M,et al.Ameliorative effect of low molecular weight peptides from the head of red shrimp (Solenocera crassicornis) against cyclophosphamide-induced hepatotoxicity in mice[J].Journal of Functional Foods,2020,72:104 085.
[19] XU G X,CUI L J,LIN Y,et al.Protective effect of haliotidis on the oxidative damage in the human lens epithelial cells[J].Chinese Journal of Ophthalmology,2013,49(9):817-821.
[20] FANG Y,SU T,QIU X R,et al.Protective effect of alpha-mangostin against oxidative stress induced-retinal cell death[J].Scientific Reports,2016,6:21 018.
[21] WANG Z C,WANG Z,HUANG W H,et al.Antioxidant and anti-inflammatory activities of an anti-diabetic polysaccharide extracted from Gynostemma pentaphyllum herb[J].International Journal of Biological Macromolecules,2020,145:484-491.
[22] HOU Y N,LI X M,PENG S J,et al.Lipoamide ameliorates oxidative stress via induction of Nrf2/ARE signaling pathway in PC12 cells[J].Journal of Agricultural and Food Chemistry,2019,67(29):8 227-8 234.
[23] LOBODA A,DAMULEWICZ M,PYZA E,et al.Role of Nrf2/HO-1 system in development,oxidative stress response and diseases:an evolutionarily conserved mechanism[J].Cellular and Molecular Life Sciences,2016,73(17):3 221-3 247.
[24] BAO L P,LI J S,ZHA D Q,et al.Chlorogenic acid prevents diabetic nephropathy by inhibiting oxidative stress and inflammation through modulation of the Nrf2/HO-1 and NF-κB pathways[J].International Immunopharmacology,2018,54:245-253.
[25] YANG S B,ZHENG Y,HOU X H.Lipoxin A4 restores oxidative stress-induced vascular endothelial cell injury and thrombosis-related factor expression by its receptor-mediated activation of Nrf2-HO-1 axis[J].Cellular Signalling,2019,60:146-153.
[26] ZHANG C Y,REN X M,LI H B,et al.Simvastatin alleviates inflammation and oxidative stress in rats with cerebral hemorrhage through Nrf2-ARE signaling pathway[J].European Review for Medical and Pharmacological Sciences,2019,23(14):6 321-6 329.
[27] FENG S,XU Z F,WANG F,et al.Sulforaphane prevents methylmercury-induced oxidative damage and excitotoxicity through activation of the Nrf2-ARE pathway[J].Molecular Neurobiology,2017,54(1):375-391.
[28] SCHMIDLIN C J, DODSON M B, MADHAVAN L,et al.Redox regulation by NRF2 in aging and disease[J].Free Radical Biology and Medicine,2019,134:702-707.
[29] ZHANG Y,WANG G Y,WANG T R,et al.Nrf2-Keap1 pathway-mediated effects of resveratrol on oxidative stress and apoptosis in hydrogen peroxide-treated rheumatoid arthritis fibroblast-like synoviocytes[J].Annals of the New York Academy of Sciences,2019,1 457(1):166-178.
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