Using Lactobacillus B2-1 to ferment whey protein, the product was separated and purified to obtain biologically active peptides with antioxidant and antitumor activities. Whey protein was fermented by Lactobacillus B2-1 under anaerobic cultivation for five weeks, and the metabolites were purified by macroporous adsorption resin D101 column chromatography and Sephadex G15 gel column chromatography. The antioxidant activity of biologically active peptides was studied by examining their ability to scavenge ABTS radical. Its antitumor activity was studied by examining the survival of colon and oral cancer cells. Molecular weight distribution was observed by polyacrylamide gel electrophoresis. The results showed that the purified fraction F3-1 was a good component with antitumor and antioxidant activities. In vitro antioxidation experiments showed that F3-1 had the best ability to eliminate ABTS free radicals. When the concentration was 8 g/L, the inhibition rate of human colon cancer LoVo cell proliferation was 75.36%. Based on these results, the Lactobacillus B2-1 can be used to ferment whey protein to obtain the biologically active peptide components with antitumor and antioxidant functions.
GUO Hao
,
DING Lin
,
FAN Lei
,
YANG Wenqin
,
JIN Jinjian
,
ZANG Chuangang
,
LIU Yuchao
,
GUAN Hong
. Study on biologically active peptides in whey protein products fermented by Lactobacillus B2-1[J]. Food and Fermentation Industries, 2021
, 47(18)
: 154
-158
.
DOI: 10.13995/j.cnki.11-1802/ts.026928
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