In this study, nobiletin nanoemulsions based on functional oils (perilla seed oil, hemp oil, and camellia oil) were successfully prepared by a high-energy method. Then, their digestive properties in the oral cavity, stomach and small intestine, including key parameters such as particle size distribution and potential changes, the digestion laws of different oil phase and the effects on the bioaccessibility of nobiletin were systematically analyzed through in vitro digestion simulation experiments. The results indicated that the three prepared nanoemulsions had similar particle size distributions and potential changes during digestion, however, the degree of oil phase digestion was significantly different. Particularly, The highest degree of digestion was camellia oil [(93.7±1.4)%], followed by hemp oil [(83.0±1.8)%], and the minimum was perilla seed oil [(76.4±0.9)%]. In terms of the effect on the bioaccessibility, the bioaccessibility of nobiletin in camellia oil nanoemulsion, perilla seed oil nanoemulsion and hemp seed oil nanoemulsion were (66.4±2.9)%, (48.0±1.5)%, and (46.0±1.0)%, respectively. These results suggested the superior effect in increasing the bioaccessibility of nobiletin by camellia oil nanoemulsion that rich in monounsaturated fatty. In conclusion, this study provides a new strategy to improve the bioaccessibility of nobiletin, and promote its industrial application.
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