The authenticity and safety of meat directly affect the health of consumers. A rapid identification method for beef in meat food using PCR-nucleic acid test strip and a rapid detection kit was developed in this paper. First, the cattle specific primers in GB were labeled, and the beef DNA rapid extraction reagents and beef gene detection reagents were developed, and nucleic acid test strips were used for visual detection. Then, molecular cloning and sequencing technologies were applied to clone standard beef DNA products. The specificity, reproducibility, stability and sensitivity of the reagents were investigated next. The method for genomic DNA extracting was simple and rapid, the integrity, concentration, and purity of DNA were excellent. The test strip showed the most specific results when annealing at 59 ℃ for 20 cycles. Two strips appeared in all the authentic beef samples, and one strip appeared in the confusing beef samples and the blank control samples. The sequence of the cloned beef DNA was 100% homologous to the specific fingerprint sequence of bovine mt DNA. The self-developed kit had good specificity, reproducibility and stability, the minimum detectable amount of template DNA was 1 pg/μL. The PCR-nucleic acid test strip method established in this study is specific, sensitive, accurate, simple and rapid. The developed kit is easy to operate, and the results are visible and stable, which is suitable for field monitoring.
JIANG Haiying
,
ZHANG Zhijie
,
WANG Yanshuang
,
ZHANG Li
,
GAO Lijun
,
LI Mingcheng
,
SUN Liyuan
,
ZHANG Lihua
. Establishment of a rapid identification method on PCR-nucleic acid strip for beef and development of kits[J]. Food and Fermentation Industries, 2021
, 47(18)
: 275
-281
.
DOI: 10.13995/j.cnki.11-1802/ts.027563
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