Food and Fermentation Industries >

2021 , Vol. 47 >Issue 19: 35 - 42

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026850

Structure and antioxidant activity of Pediococcus lactis extracellular polysaccharide

  • LI Yao ,
  • LU Chengrong ,
  • LIU Dan ,
  • HAN Xiangpeng ,
  • ZHONG Qingping
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  • (College of Food Science,South China Agricultural University,Guangzhou 510642,China)

Received date: 2021-01-30

  Revised date: 2021-03-09

  Online published: 2021-11-04

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Abstract

Anion exchange chromatography and molecular exclusion chromatography were used to purify the extracellular polysaccharide (EPS) from Pediococcus lactis C6. EPS1, EPS2 and EPS3 were obtained. These three components possessed antioxidant capacities with the scavenging effects on DPPH, ABTS, OH, and ·O-2. EPS3 and EPS2 presented stronger antioxidant activities than EPS1. The molecular weights of EPS2 and EPS3 were 1.90×104, and 2.53×104 Da, respectively. The ion chromatography analysis indicated that EPS2 composed of xylose, glucosamine hydrochloride, galactose and glucose, and the molar ratios were 0.912∶0.016∶0.022∶0.051. EPS3 composed of glucosamine hydrochloride, galactose, glucose, xylose, galacturonic acid and glucuronic acid, and the molar ratios were 0.105∶0.231∶0.471∶0.170∶0.010∶0.013. The infrared spectroscopy scans demonstrated that there were functional sugar groups such as pyran ring, carbonyl group and hydroxyl group in EPS2 and EPS3. The nuclear magnetic resonance analysis showed that EPS2 and EPS3 were all pyranose rings, and presented as α or β configuration.

Key words： Pediococcus lactis; extracellular polysaccharide; structure analysis; antioxidant

Cite this article

LI Yao , LU Chengrong , LIU Dan , HAN Xiangpeng , ZHONG Qingping . Structure and antioxidant activity of Pediococcus lactis extracellular polysaccharide[J]. Food and Fermentation Industries, 2021 , 47(19) : 35 -42 . DOI: 10.13995/j.cnki.11-1802/ts.026850

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