Combining qualitative and quantitative methods, a simple absorption spectrometric method for the determination of coexisting pigments in several carbonated beverages—brilliant blue, tartrazine and sunset yellow was established. The direct method was to measure bright blue under the following conditions: maximum absorption wavelength was 628 nm and the apparent molar absorption coefficient (κ) was 1.20×105 L/(mol·cm). The detection limit reached 0.018 mg/L, the linear range was 0.02-15.4 mg/L and the quantitative limit of beverages was 0.31 mg/kg. Moreover, the acidity control method was used to determine tartrazine and sunset yellow (bright blue didn't interfere). Under the condition of pH 3.50 Tris-HCl, brilliant green reacted with tartrazine and sunset yellow to form an ionic association complex. The maximum absorption wavelength of the tartrazine system was 426 nm, the κ was 2.59×104 L/(mol·cm), the detection limit was 0.028 mg/L, the linear range was 0.03-13.4 mg/L, and the quantitative limit of beverage was 0.47 mg/kg. In addition, the maximum absorption wavelength of the sunset yellow system was 500 nm, the κ was 1.46×104 L/(mol·cm), the detection limit was 0.030 mg/L, the linear range was 0.03-8.14 mg/L, and the quantitative limit of beverage was 0.51 mg/kg. This method developed here was simple and rapid for the determination of coexisting pigments in beverages—brilliant blue, tartrazine and sunset yellow, with satisfactory results.
JIANG Hong
,
PANG Xiangdong
. Combination of direct photometry and acidity control photometry to determine coexisting pigments in carbonated drinks[J]. Food and Fermentation Industries, 2021
, 47(19)
: 271
-276
.
DOI: 10.13995/j.cnki.11-1802/ts.027006
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