Food and Fermentation Industries >

2021 , Vol. 47 >Issue 20: 44 - 51

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026454

Protective effects of plant fermentation extracts on alcohol liver injury in mice

  • LIU Chunhua ,
  • ZHANG Yifan ,
  • WANG Pu ,
  • LI Xiang ,
  • LIANG Yan
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  • 1(School of Resources and Environment, University of Electronic Science and Technology of China, Chengdu 611731, China)
    2(Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China)
    3(College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China)

Received date: 2020-12-16

  Revised date: 2021-02-22

  Online published: 2021-11-18

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Abstract

Protective effects of plant fermentation extracts (PFE) against alcohol-induced liver injury in mice was investigated. Male C57BL/6 mice were randomly divided into four groups. The normal control group and the model group were given distilled water per day, and the PFE low and high dose groups were given the corresponding PFE (1.25 or 5.00 mL/kg·BW). After 1 h, all groups were given 56 %vol liquor (10 mL/kg·BW) except the normal control group. The serum liver function indexes, cytokine levels, key enzyme activities of hepatic ethanol metabolism, antioxidant indexes and mRNA expression levels of related genes were detected, and the pathological changes of liver tissues were observed. PFE administration with ethanol resulted in prevention of ethanol-induced hepatotoxicity due to reductions of serum aspartate aminotransferase (AST) and alanine transaminase (ALT) levels in mice treated with PFE for 8, 10,12 weeks. Results of hematoxylin-eosin staining showed that administration of PFE significantly alleviated alcohol-induced hepatocellular lesions in mice. Alcohol-induced upregulation of alcohol dehydrogenase (ADH), aldehyde dehydrogenase (ALDH) activity and downregulation of recombinant cytochrome P450 2E1 (Cyp2e1) mRNA expression in liver tissue were dramatically inhibited by PFE treatment. Mice treated with PFE showed increased hepatic antioxidant system with normal activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC), and reduced formation of malondialdehyde (MDA) in liver. Furthermore, PFE treatment also significantly inhibited the alcohol-induced inflammatory by increasing the hepatic interleukin-6 (Il6) mRNA expression and serum interleukin-1β (IL-1β) level, and the alcohol-induced increase of signal transducer and activator of transcription 3 (Stat3) mRNA expression. This study showed that PFE exerted hepatoprotective effects against alcohol-induced hepatic injury by mediating ethanol metabolism, oxidation and inflammation.

Key words： plant fermentation extracts (PFE); alcohol liver injury; antioxidant activity; anti-inflammatory

Cite this article

LIU Chunhua , ZHANG Yifan , WANG Pu , LI Xiang , LIANG Yan . Protective effects of plant fermentation extracts on alcohol liver injury in mice[J]. Food and Fermentation Industries, 2021 , 47(20) : 44 -51 . DOI: 10.13995/j.cnki.11-1802/ts.026454

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