Bacillus cereus is a common food-borne pathogen and anti-SigP factor RsiP is an important regulator of penicillin resistance. B. cereus BC307 strain from the food residues of food poisoning patient was isolated, which has strong resistance to penicillin. The RsiP protein was expressed and purified by affinity chromatography. High-titer polyclonal antibody was obtained after immunizing mice. The results showed that the RsiP protein was highly conserved and identical in B. anthracis, B. cereus, and B. thuringiensis. Western blotting showed that the RsiP antiserum had specific immunological reactions with the whole bacterial protein of these three bacteria. The studies lay a good foundation for exploring the regulation of penicillin resistance of B. cereus by RsiP.
ZHENG Qingfang
,
LIU Lina
,
LIU Xiankai
,
LYU Yufei
,
WANG Hengliang
,
WANG Dongshu
,
QIU Zewu
. Identification of Bacillus cereus BC307 and the antibody preparation against RsiP[J]. Food and Fermentation Industries, 2021
, 47(21)
: 53
-58
.
DOI: 10.13995/j.cnki.11-1802/ts.027084
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