To improve the biomass of recombinant Kluyveromyces lactis GG799(pKLAC1-ZPF1), the optimal high-density cultivation conditions was conducted. The optimal conditions were verified in a 5 L fermentor. The results showed that the optimized culture composition was as follows: 20.41 g/L of glucose, 30.46 g/L of peptone, 24.73 g/L of yeast extract, 4.48 g/L of KH2PO4 and 0.65 g/L of MgSO4, and the biomass was 5.5 times of control. While the optimized cultivation parameters were as follows: 26℃, 220 r/min and the initial pH 4.5, and the biomass were 7.5 times of control. The biomass was (34.05±3.34) g/L in 5 L fermentor, which was 29.6 times of control. The results provided important theoretical basis and reference data for the future industrial production of K. lactis.
XIA Yu
,
WU Zifeng
,
HU Xiuyu
,
BAO Hongduo
,
HE Rui
,
QIU Yangyu
,
CHENG Qianqian
,
WANG Zhouping
. Optimal cultivation conditions for food-grade recombinant yeast that simultaneously degrades two mycotoxins[J]. Food and Fermentation Industries, 2021
, 47(21)
: 86
-93
.
DOI: 10.13995/j.cnki.11-1802/ts.028751
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