The identification of the species origin is of great significance in dairy products adulteration detection. In order to detect four common animal species (bovine, buffalo, goat and sheep) in milk and dairy products, a PCR high-resolution melting (HRM) detection method was established based on universal primers detection. By analyzing the melting curve of the universal primer-generated amplicons, PCR-HRM was capable of identifying the mixtures of various animal-derived components, and also of sensitively detecting the presence of bovine milk in goat milk and buffalo milk down to 5%. In addition, it could accurately identify the species origin of commercial milk and dairy products. The proposed method is rapid, sensitive, high throughput, and can realize the identification of bovine, buffalo, goat and sheep with only one pair of primer. It is an effective method for the identification of milk derived constituents. It could be further applied to identify the above four animal species in various meat and meat products or other foods.
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