In this study, Lactobacillus bulgaricus, Streptococcus thermophilus, Lactobacillus plantarum and Lactobacillus paracasei were used to ferment whey protein and blueberry juice mixture system.The bacteriostatic effect of the fermentation system on three pathogenic bacteria (Staphylococcus aureus, Bacillus subtilis and Salmonella) and the mechanism action on the three pathogenic bacteria were investigated.This study provided a theoretical basis for the whey protein and blueberry juice mixture of lactic acid bacteria fermentation system as a new natural antibacterial agent.It showed that blueberry juice and whey protein mixture of lactic acid bacteria fermentation system has certain inhibition effects on the pathogenic bacteria.The blueberry juice lactic acid bacteria fermentation system was mainly breaking cell wall and cell membrane integrity of pathogenic bacteria, the permeability of suppressing.This means that the blueberry juice fermentation system was mainly characterized by the sterilization effect.The fermentation system of blueberry juice and whey protein mixture was mainly bacteriostatic.
[1] 韩鹏祥, 张蓓, 冯叙桥, 等.蓝莓的营养保健功能及其开发利用[J].食品工业科技, 2015, 36(6):370-375;379.
HAN P X, ZHANG B, FENG X Q, et al.Nutritional and health functions of blueberry and its development and utilization[J].Science and Technology of Food Industry, 2015, 36(6):370-375;379.
[2] 李兴元. 蓝莓花青素、多酚类物质的分离纯化与生物活性研究[D].天津:天津大学, 2012.
LI X Y.Investigation on separation, purification and biological activity of anthocyanins and polyphenols in blueberry[D].Tianjin:Tianjin University, 2012.
[3] 孙希云, 赵秀红, 张琦, 等.红树莓花色苷粗提物抗氧化性能与抑菌作用研究[J].食品工业科技, 2009,30(3):132-135.
SUN X Y, ZHAO X H, ZHANG Q, et al.Study on the ability to antioxidantion and bacteriostasis of crude extract of red raspberry anthocyanins[J].Science and Technology of Food Industry, 2009,30(3):132-135.
[4] ZHOU T T, WEI C H, LAN W Q, et al.The effect of Chinese wild blueberry fractions on the growth and membrane integrity of various foodborne pathogens[J].Journal of Food Science, 2020, 85(5):1 513-1 522.
[5] LACOMBE A, WU V C H.The potential of berries to serve as selective inhibitors of pathogens and promoters of beneficial microorganisms[J].Food Quality and Safety, 2017, 1(1):3-12.
[6] LACOMBE A, WU V, TYLER S, et al.Antimicrobial action of the American cranberry constituents;phenolics, anthocyanins, and organic acids, against Escherichia Coli O157:H7[J].International Journal of Food Microbiology, 2010, 139 (1-2):102-107.
[7] 王亚丽, 李颖畅, 马春颖, 等.蓝莓叶多酚提取物对3种细菌的抑菌活性[J].食品与发酵工业, 2015, 41(3):163-167.
WANG Y L, LI Y C, MA C Y, et al.Study on antimicrobial activity of blueberry leaf polyphenol against three microorganisms[J].Food and Fermentation Industries, 2015, 41(3):163-167.
[8] 陈嘉琪, 崔树茂, 唐鑫, 等.德式乳杆菌保加利亚亚种的乳清蛋白利用能力比较[J].食品与发酵工业, 2020, 46(7):28-34.
CHEN J Q, CUI S M, TANG X, et al.Comparison of whey protein hydrolytic ability of Lactobacillus delbrueckii subsp.bulgaricus[J].Food and Fermentation Industries, 2020, 46(7):28-34.
[9] YADAV J S S, YAN S, PILLI S, et al.Cheese whey:A potential resource to transform into bioprotein, functional/nutritional proteins and bioactive peptides[J].Biotechnology Advances, 2015, 33(6):756-774.
[10] 王文琼, 孙志勇, 黄冬成, 等.乳酸菌发酵蓝莓乳清混合体系体外抗氧化特性分析[J].现代食品科技, 2021, 37 (1):142-149.
WANG W Q, SUN Z Y, HUANG D C, et al.Antioxidant properties analysis in vitro of lactic acid bacteria fermentation blueberry and whey mixture system[J].Modern Food Science and Technology, 2021, 37 (1):142-149.
[11] 王文琼, 孙志勇, 黄冬成, 等.乳酸菌发酵对乳清蛋白蓝莓果汁体系特性的影响[J].食品与发酵工业, 2020, 46(13):42-48.
WANG W Q, SUN Z Y, HUANG D C, et al.Effect of lactic acid bacteria fermentation on the characteristics of whey protein /blueberry juice system[J].Food and Fermentation Industries, 2020, 46(13):42-48.
[12] 高鹏. 广谱抗菌乳酸菌的分离鉴定及细菌素的提取、纯化及结构鉴定[D].南京:南京农业大学, 2016.
GAO P.Isolation and identification of LAB strains with a broad-spectrum antibacterial activity and extraction purification and identification of the bacteriocin[D].Nanjing:Nanjing Agricultural University, 2016.
[13] 陈华, 彭浩, 胡方兴, 等.德氏乳杆菌保加利亚亚种发酵产物对常见病原菌的抑制作用[J].国际检验医学杂志, 2020, 41(23):2 899-2 900; 2 907.
CHEN H, PENG H, HU F X, et al.Inhibitory effect of fermentation products of Lactobacillus delbrueckii subsp.bulgaricus on common pathogens[J].International Journal of Laboratory Medicine, 2020, 41(23):2 899-2 900; 2 907.
[14] ABEDI D, FEIZIZADEH S, AKBARI V, et al.In vitro anti-bacterial and anti-adherence effects of Lactobacillus delbrueckii subsp. bulgaricus on Escherichia coli[J].Research in Pharmaceutical Sciences, 2013, 8(4):260-268.
[15] ARENA M P, SILVAIN A, NORMANNO G, et al.Use of Lactobacillus plantarum strains as a bio-control strategy against food-borne pathogenic microorganisms[J].Front Microbiol, 2016, 7:464.
[16] PERAL M C, HUAMAN MARTINEZ M A, VALDEZ J C.Bacteriotherapy with Lactobacillus Plantarum in burns[J].International Wound Journal, 2009, 6(1):73-81.
[17] 孙小涵, 孙洪浩, 陈秀秀, 等.具有抑菌活性植物乳杆菌的筛选及抑菌物质的特性分析[J].养猪, 2020(6):18-20.
SUN X H, SUN H H, CHEN X X, et al.Screening of Lactobacillus plantarum with antimicrobial activity and characterization of antimicrobial substances[J].Swine Production, 2020(6):18-20.
[18] GUO J H, BROSNAN B, FUREY A, et al.Antifungal activity of Lactobacillus against Microsporum canis, Microsporum gypseum and Epidermophyton floccosum[J].Bioengineered, 2012, 3(2):104-113.
