Food and Fermentation Industries >

2021 , Vol. 47 >Issue 22: 249 - 253

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027296

Rapid detection of amantadine by fluorescence sensing technology based on nucleic acid aptamer-SYBR Green Ⅰ

  • LYU Chen ,
  • LI Yujin ,
  • SONG Mingyan ,
  • WU Shijia ,
  • DUAN Nuo ,
  • WANG Zhouping
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  • 1(School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)
    2(College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China)

Received date: 2021-03-09

  Revised date: 2021-04-13

  Online published: 2021-12-16

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Abstract

Based on the nucleic acid aptamer technology and using the SYBR Green I fluorescent dye as the signal molecule, a new label-free fluorescent sensing method for rapid detection of amantadine was established, which could detect trace amantadine residues in animal-derived foods. Based on the specific recognition between the aptamer and amantadine, the fluorescence intensity of the aptamer-SYBR Green I before and after binding to the target was analyzed to achieve the quantitative detection of amantadine. The results showed that the method had a good linear relationship(R2=0.992 5)in the concentration range of 1 and 100 ng/mL and the detection limit was 0.84 ng/mL. The recovery rate was between 93.22% and 104.86% in the detection of milk samples, which showed good practicability. The method was simple, sensitive and specific, and provided a new idea for the determination of veterinary drug residues in food.

Key words： amantadine; aptamer; SYBR Green I; fluorescence sensing technology; rapid detection

Cite this article

LYU Chen , LI Yujin , SONG Mingyan , WU Shijia , DUAN Nuo , WANG Zhouping . Rapid detection of amantadine by fluorescence sensing technology based on nucleic acid aptamer-SYBR Green Ⅰ[J]. Food and Fermentation Industries, 2021 , 47(22) : 249 -253 . DOI: 10.13995/j.cnki.11-1802/ts.027296

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