Food and Fermentation Industries >

2021 , Vol. 47 >Issue 22: 266 - 272

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027325

Simultaneous determination of six phenols in garlic skin by solid phase extraction-high performance liquid chromatography

  • GAO Ruorong ,
  • WANG Tianlie ,
  • HUANG Xuesong
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  • 1(Food Science and Engineering, Jinan University, Guangzhou 510632, China)
    2(Guangdong Xinyue Food Co.Ltd., Chaozhou 515638, China)

Received date: 2021-03-15

  Revised date: 2021-04-14

  Online published: 2021-12-16

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Abstract

A simple and sensitive solid-phase extraction-high performance liquid chromatography method was established for the analysis of 6 kinds of phenolic substance such as caffeic acid, ferulic acid, p-coumaric acid, N-trans-coumaroyloctopamine (CO) and guaiacylglycerol-β-caffeic acid ether (GCAE), guaiacylglycerol-β-ferulic acid ether (GFAE) from garlic skin. The garlic skin powder was ultrasonically extracted with 80% ethanol aqueous solution. The obtained crude polyphenol extract was loaded in a DPA-6S solid-phase extraction cartridge which was washed with ultra-pure water. The adsorbed components in the cartridge were then eluted with 70% methanol aqueous solution, dried under the rotary evaporator and dissolved in methanol so as to produce the garlic skin polyphenol sample. Using 0.1% formic acid aqueous solution and acetonitrile as the mobile phase, the sample was separated by C18 chromatographic column under gradient elution condition, combined with high-resolution mass spectrometry, standards, etc. for the qualitative detection of phenolic substances in the sample, and quantitative analysis by external standard method. The results showed that the garlic skin contained caffeic acid; under the optimal gradient elution method, the components were well separated with the correlation coefficient 0.999 9. The detection limit was 0.33-0.66 mg/L, and the limit of quantification was 1.26-2.32 mg/L, the recovery of standard addition was 82.79%-100.58%, and the relative standard deviation was less than 1%. The contents of caffeic acid, ferulic acid, p-coumaric acid and CO in garlic skin were: 4.99, 9.15, 2.96, 7.27 mg/kg respectively, the contents of GCAE and GFAE were 32.89 and 8.11 mg/kg (calculated as caffeic acid and ferulic acid respectively). The total content of 6 phenolic substances was 65.37 mg/kg. The method is simple, fast, and highly sensitive, and is suitable for the determination of six phenolic substances in garlic skin.

Key words： garlic skin; solid-phase extraction; high-performance liquid chromatography; caffeic acid; phenols

Cite this article

GAO Ruorong , WANG Tianlie , HUANG Xuesong . Simultaneous determination of six phenols in garlic skin by solid phase extraction-high performance liquid chromatography[J]. Food and Fermentation Industries, 2021 , 47(22) : 266 -272 . DOI: 10.13995/j.cnki.11-1802/ts.027325

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