Food and Fermentation Industries >

2021 , Vol. 47 >Issue 23: 61 - 69

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028686

Effects of structural characteristics and antioxidant activity of collagen bioactive peptides from Takifugu obscurus skin during simulated gastrointestinal digestion

  • CAO Zhenhai ,
  • LE Caihong ,
  • TAO Ningping ,
  • DENG Shanggui
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  • 1(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China)
    2(Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation,Shanghai 201306,China)
    3(College of Food and Pharmacy,Zhejiang Ocean University,Zhoushan 316000,China)
    4(Zhejiang Xingye Group Co.Ltd.,Zhoushan 316000,China)

Received date: 2021-07-27

  Revised date: 2021-08-16

  Online published: 2021-12-31

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Abstract

To clarify the structural characteristics of fish collagen bioactive peptides in the gastrointestinal digestion process and its antioxidant activity, the model of INFOGEST static in vitro simulation was used to analyze the collagen peptides of Takifugu obscurus skin collagen peptide (TOSCP) with a molecular weight distribution of less than 1 kDa and commercial fish skin collagen peptide (CFSCP) mainly distributed in the part with molecular weight higher than 1 kDa. The structural characteristics (microstructure, molecular weight, secondary structure and free amino acid release rate) of collagen bioactive peptides and antioxidant activity were analyzed in different digestion stages. The results showed that in the stage of simulated gastric digestion, pepsin was mainly broken from large peptides to small peptides, and had a stronger preference for high molecular weight collagen bioactive peptides (>1 kDa). TOSCP and CFSCP presented a honeycomb network structure after intestinal digestion, in which the ratio of <1 kDa was 85.39% and 52.55%, respectively. The peptides were gradually transformed into the α-helical structure under the induction of gastrointestinal enzymes, and proline and hydroxyproline showed strong resistance to digestion. TOSCP showed stronger antioxidant activity in the stomach, DPPH free radical clearance rate and total reducing power were 86.42% and 0.69%, respectively. Besides, TOSCP with smaller molecular weight was always higher than CFSCP. Therefore, TOSCP of small molecular weight distribution showed better digestibility and biological stability in gastrointestinal digestion, and Takifugu obscurus skin was an ideal raw material for active protein peptides. This research can provide an experimental basis for the research and development of collagen peptide functional products.

Key words： Takifugu obscurus; collagen bioactive peptide; in vitro digestion; structural characteristics; antioxidant activity

Cite this article

CAO Zhenhai , LE Caihong , TAO Ningping , DENG Shanggui . Effects of structural characteristics and antioxidant activity of collagen bioactive peptides from Takifugu obscurus skin during simulated gastrointestinal digestion[J]. Food and Fermentation Industries, 2021 , 47(23) : 61 -69 . DOI: 10.13995/j.cnki.11-1802/ts.028686

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