A method using one-dimensional proton nuclear magnetic resonance (1H NMR) has been established to quantify lactose content in milk products by using citric acid and nicotinamide as the quantitative external and internal standard. The results showed that the detection limit and quantitative limit were 0.05 g/L (S/N=3) and 0.2 g/L(S/N=10) respectively. The linear relationship was good in the range of 0.05-25.60 g/L(R2=0.99). The recovery of external standard method for spiked sample was 95.2%, the intra-day and inter-day precision of this method were 0.58% and 0.56%, respectively. The recovery of internal standard method for sample spiked was 97.6%, the intra-day and inter-day precision of this method were 0.42% and 0.94% respectively. And the intra-day and inter-day precision relative standard deviation (RSD) of 1H NMR method were less than 1%. The error between NMR and HPLC was less than ±5%, which met the requirements of feasibility comparative analysis and validation. Compared with HPLC method, 1H NMR simplified the sample pretreatment procedure, which saved time and had high detection efficiency and sensitivity. It can be widely used in the quality control and adulteration identification of milk products.
FAN Shuangxi
,
LIU Yinuo
,
JI Xin
,
CHANG Di
,
FENG Cuiping
,
WU Zhuying
,
ZHONG Qiding
. Quantitative determination of lactose in milk products by nuclear magnetic resonance[J]. Food and Fermentation Industries, 2021
, 47(23)
: 227
-232
.
DOI: 10.13995/j.cnki.11-1802/ts.025303
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