Screening,identification and fermentation characteristics of yeast with high-yield of ester

  • LIU Wei ,
  • LUAN Chunguang ,
  • WANG Deliang ,
  • JIANG Xin
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  • 1(College of Food Science and Pharmacy,Xinjiang Agricultural University,Urumqi 830052,China)
    2(China National Research Institute of Food and Fermentation Industries Co.Ltd.,Beijing 100015,China)

Received date: 2021-05-24

  Revised date: 2021-08-29

  Online published: 2021-12-31

Abstract

Two high-yield ethyl acetate strains were isolated from vinegar koji and Daqu,named Y10 and Y12,and identified as Wickerhamomyces anomalus by molecular biology identification.Using sorghum juice fermentation preliminary screening,the output of ethyl acetate for Y10 and Y12 reached 6.68 g/L and 4.73 g/L respectively.The fermentation performance of the two yeast strains showed that both of them had good tolerance to glucose,ethanol,pH,and temperature.The conditions for producing ethyl acetate in the two strains were optimized by single factor and three factor three-level orthogonal experiment.The results showed that the optimized conditions for strains Y10 and Y12 were:ethanol concentration 2%,pH 6 and 28 ℃.The highest yield of ethyl acetate reached 13.18 g/L and 12.92 g/L,the content of ethyl acetate had been significantly increased by 4.17 g/L and 5.87 g/L compared with that of before,showing good practical application potential.

Cite this article

LIU Wei , LUAN Chunguang , WANG Deliang , JIANG Xin . Screening,identification and fermentation characteristics of yeast with high-yield of ester[J]. Food and Fermentation Industries, 2021 , 47(23) : 311 -318 . DOI: 10.13995/j.cnki.11-1802/ts.029783

References

[1] 张天震,孙晓琪,刘丹彤,等.山西老陈醋大曲细菌群落结构及多样性研究[J].中国食品学报,2021,21(5):323-330.
ZHANG T Z,SUN X Q,LIU D T,et al.Studies on bacterial community structure and diversity in Daqu from Shanxi aged vinegar[J].Journal of Chinese Institute of Food Science and Technology,2021,21(5):323-330.
[2] 刘丹彤,刘峰,康帅帅,等.传统山西老陈醋熏醅工艺过程风味物质及功能活性成分的变化[J].中国酿造,2021,40(2):163-169.
LIU D T,LIU F,KANG S S,et al.Changes of flavor substances and functional active components in fumigation process of traditional Shanxi aged vinegar[J].China Brewing,2021,40(2):163-169.
[3] WANG Z M,LU Z M,SHI J S,et al.Exploring flavour-producing core microbiota in multispecies solid-state fermentation of traditional Chinese vinegar[J].Scientific Reports,2016,6:26818.
[4] 范梦蝶,王天泽,杜文斌,等.山西老陈醋晾晒前、后挥发性香味物质比较分析[J].中国食品学报,2019,19(12):229-242.
FAN M D,WANG T Z,DU W B,et al.Comparison of volatile flavor compounds in Shanxi aged vinegar before and after aging[J].Journal of Chinese Institute of Food Science and Technology,2019,19(12):229-242.
[5] CHEN J,XING X Y,FENG Z H,et al.Screening and aroma characteristics of ester-producing yeasts as starter cultures for Shanxi aged vinegar[J].Food Science,2019,40(16):144-151.
[6] ZHENG X W,YAN Z,NOUT M J R,et al.Microbiota dynamics related to environmental conditions during the fermentative production of Fen-Daqu,a Chinese industrial fermentation starter[J].International Journal of Food Microbiology,2014,182-183:57-62.
[7] 李婷,张磊,杨宇霞,等.山西老陈醋熏醅阶段风味物质的动态变化[J].中国调味品,2020,45(8):65-68.
LI T,ZHANG L,YANG Y X,et al.Dynamic changes of flavor substances of Shanxi aged vinegar during fumigation process[J].China Condiment,2020,45(8):65-68.
[8] 程伟,彭兵,汪焰胜,等.清香型酒醅中一株产乙酸乙酯酵母菌的筛选及其应用性能分析[J].酿酒,2018,45(5):43-47.
CHENG W,PENG B,WANG Y S,et al.Screening of one yeast strain with high-yield of ethyl acetate from Fen-flavor liquor fermenting-grains and analysis of its application performance[J].Liquor Making,2018,45(5):43-47.
[9] 陈嘉,冯志宏,施俊凤.传统山西老陈醋增香酵母菌的筛选及其特性研究[J].保鲜与加工,2020,20(1):120-126.
CHEN J,FENG Z H,SHI J F.Screening and characteristics study of flavoring yeast from traditional Shanxi aged vinegar[J].Storage and Process,2020,20(1):120-126.
[10] 董凯锋,安娜,李东乐,等.水塔老陈醋大曲酵母的分离鉴定及产乙醇和乙酸乙酯特性[J].食品工业科技,2016,37(10):213-216.
DONG K F,AN N,LI D L,et al.Isolation and identification of yeast in Daqu for aged vinegar production and their capacity for producing ethly alcohol and ethly acetate[J].Science and Technology of Food Industry,2016,37(10):213-216.
[11] 张杰,程伟,巩子路,等.一株高产乙酸乙酯酵母菌的筛选及其鉴定[J].酿酒,2018,45(6):59-62.
ZHANG J,CHENG W,GONG Z L,et al.Screening and identification of a strain with high yield of ethyl acetate yeast[J].Liquor Making,2018,45(6):59-62.
[12] 唐洁.清香型小曲酒微生物群落结构及功能的研究[D].江南大学,2012.
TANG J.Characterization of the structure and function of the microbial community in light aroma type liquor started by Xiaoqu[D].Wuxi:Jiangnan University,2012.
[13] 崔海灏.十里香酒产酯酵母的筛选及应用研究[D].保定:河北大学,2020.
CUI H H.A study on the screening and application of ester-producing yeast from Shilixiang[D].Baoding:Hebei University,2020.
[14] 王媛慧.山西老陈醋大曲中优良酵母菌的分离鉴定及发酵应用研究[D].太谷:山西农业大学,2014.
WANG Y H.Screening and identification of quality yeast strains in daqu of Shanxi-super vinegar and research on fermentation[D].Taigu:Shanxi Agricultural University,2014.
[15] 邢晓莹,刘毅,霍乃蕊,等.山西老陈醋中优良酵母菌株筛选及其在木枣果酒发酵中的应用[J].中国酿造,2021,40(3):64-72.
XING X Y,LIU Y,HUO N R,et al.Screening of excellent yeast strains in Shanxi-aged vinegar and their application in jujube wine fermentation[J].China Brewing,2021,40(3):64-72.
[16] 查枢屏,李兰,董琪,等.古井贡大曲中高产酯酵母的分离及分子学鉴定[J].酿酒,2017,44(4):45-48.
ZHA S P,LI L,DONG Q,et al,Isolation and molecular identification of high ester yeast Gujinggong Daqu[J].Liquor Making,2017,44(4):45-48.
[17] 王鹏昊,关统伟,张习超,等.小曲中优质产酯酵母分离鉴定及其产酯条件优化[J].食品与发酵工业,2018,44(2):62-67.
WANG P H,GUAN T W,ZHANG X C,et al.Isolation and identification of efficient ester-producing yeast in Xiaoqu and optimization of ester production conditions[J].Food and Fermentation Industries,2018,44(2):62-67.
[18] 李志辉,任蓓蕾,朱健辉,等.一株产乙酸乙酯酵母C42的分离与鉴定[J].食品工业科技,2014,35(8):188-191.
LI Z H,REN B L,ZHU J H,et al.Isolation and identification of an ethyl acetate producing yeast strain C-42[J].Science and Technology of Food Industry,2014,35(8):188-191.
[19] 王晓丹,韩超,印丽,等.白酒酿造过程中耐高糖酵母的筛选及发酵产多元醇分析[J].中国酿造,2020,39(8):54-58.
WANG X D,HAN C,YIN L,et al.Screening of high-sugar-tolerant yeast in Baijiu brewing and analysis of polyhydric alcohols production by fermentation[J].China Brewing,2020,39(8):54-58.
[20] 刘建学,刘金科,李璇,等.白酒酒醅高产酯酵母筛选鉴定及其发酵性能研究[J].食品与机械,2020,36(6):42-49.
LIU J X,LIU J K,LI X,et al.Screening,identification and fermentation performance of a high-yield yeast from fermented grains of Dukang wine[J].Food&Machinery,2020,36(6):42-49.
[21] 史雁飞.高产乙酸乙酯且有耐受性酵母的选育及固定化研究[D].临汾:山西师范大学,2019.
SHI Y F.Breeding and immobilization of high-yield ethyl acetate and tolerant Saccharomyces cerevisiae[D].Linfen:Shanxi Normal University,2019.
[22] 刘兴艳,潘军,顾一洪,等.四株野生酵母菌株耐受性的研究[J].食品工业科技,2015,36(1):149-153;165.
LIU X Y,PAN J,GU Y H,et al.Stress tolerances of four wild yeast strains[J].Science and Technology of Food Industry,2015,36(1):149-153;165.
[23] 王朝阳.清香型大曲白酒产酯酵母的筛选及鉴定[D].太原:山西大学,2019.
WANG C Y.Screening and application of ester-producing yeast from Fen-flavor Chinese Baijiu[D].Taiyuan:Shanxi University,2019.
[24] 徐丽萍.泸型大曲中产酯酵母的筛选鉴定及产酯条件优化[J].中国酿造,2016,35(2):74-78.
XU L P.Screening and identification of ester-producing yeast in Luzhou Daqu and optimization of ester production conditions[J].China Brewing,2016,35(2):74-78.
[25] 刘源才,郭圣祥,李锐利,等.高产乙酸乙酯酵母的产酯条件研究[J].食品与发酵科技,2011,47(2):22-24.
LIU Y C,GUO S X,LI R L,et al.Researching the ester production conditions of the high production ethyl acetate yeast[J].Food and Fermentation Technology,2011,47(2):22-24.
[26] CHEN M B,LI R L,PENG N,et al.Screening of higher yield of ethyl acetate yeast and researching of ester-producing conditions[J].African Journal of Microbiology Research,2011,5(12):1 383-1 388.
[27] FU Z L,SUN B G,LI X T,et al.Isolation and characterization of a high ethyl acetate-producing yeast from Laobaigan Daqu and its fermentation conditions for producing high-quality Baijiu[J].Biotechnology & Biotechnological Equipment,2018,32(5):1 218-1 227.
[28] 李锐利.高产乙酸乙酯酵母的选育及在清香型小曲酒中的应用研究[D].武汉:湖北工业大学,2011.
LI R L.Breeding of high-yield ethylacetate yeast and research of application in Fen-flavour Xiaoqu liquor[D].Wuhan:Hubei University of Technology,2011.
[29] 李梦琦,赵一凡,郑飞云,等.耐高糖产香酵母菌的分离鉴定及其应用[J].食品与发酵工业,2019,45(24):45-51.
LI M Q,ZHAO Y F,ZHENG F Y,et al.Isolation,identification and application of sugar-tolerant and aroma-producing yeast[J].Food and Fermentation Industries,2019,45(24):45-51.
[30] 陈雪,孟勤燕,齐欢,等.高产乙酸乙酯酵母菌株的筛选[J].酿酒科技,2020(1):96-102.
CHEN X,MENG Q Y,QI H,et al.Screening of yeast strains with high-yield of ethyl acetate[J].Liquor-Making Science & Technology,2020(1):96-102.
[31] 黄光建,徐学锋,郭梅君,等.豉香型白酒酒饼中高产乙酸乙酯酵母菌的分离鉴定及发酵性能研究[J].食品工业科技,2015,36(11):153-158.
HUANG G J,XU X F,GUO M J,et al.Isolation and identification of a high-yield ethyl acetate yeast from Xiaoqu of soybean-Flavor liquor and its fermentation characterization[J].Science and Technology of Food Industry,2015,36(11):153-158.
[32] 吴嘉.海藻糖对酿酒酵母1912菌株酒精耐受性及发酵影响的研究[D].昆明:云南大学,2012.
WU J.Effects of trehalose on ethanol tolerance and fermentation in Saccharomyces cerevisiae 1912[D].Kunming:Yunnan University,2012.
[33] 匡钰,廖留萍,沈福英,等.克鲁维毕赤酵母S23发酵产香条件优化及发酵液挥发性成分分析[J].食品科技,2019,44(11):23-29.
KUANG Y,LIAO L P,SHEN F Y,et al.Optimization of aroma-producing conditions and analysis of fermentation fluid volatile components of Pichia kluyveri S23[J].Food Science and Technology,2019,44(11):23-29.
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