The ability of utilizing stachyose was determined for 32 strains of Bifidobacterium and Lactobacillus, 15 strains were found to utilize stachyose, including Bifidobacterium breve, Bifidobacterium dentium, Bifidobacterium longum ssp. longum, Bifidobacterium pseudocatenulatum, Lactobacillus fermentum, Lactobacillus paragasseri, Lactobacillus reuteri, Lactobacillus ruminis and Lactobacillus salivarius. By analyzing the content of stachyose and raffinose in the bacterial culture supernatant, the activity of α-galactosidase and the draft genome, the differences in the utilization of stachyose between Bifidobacterium and Lactobacillus were found. Some Bifidobacterium strains could intake stachyose by msmEFG transporter, which was then hydrolyzed by the enzymes such as α-galactosidase, β-fructofuranosidase, α-glucosidase and oligo-1,6-glucosidase. Some Lactobacillus could intake stachyose by permease or ABC (ATP-binding cassette) transport system and then hydrolyzed in the cell. Some bacteria had extracellular α-galactosidase, which could hydrolyze stachyose outside the cell initially for further utilization, especially B. longum ssp. longum L28 and L. reuteri L17. This study focused on the characteristics of stachyose utilization by Bifidobacterium and Lactobacillus, which will be valuable for the future research on the relationships between stachyose and human health.
WU Jiaying
,
CHEN Minxuan
,
JIN Tianci
,
WANG Shunhe
,
CUI Shumao
,
TANG Xin
,
MAO Bingyong
. Utilization characteristics of stachyose by Bifidobacterium and Lactobacillus[J]. Food and Fermentation Industries, 2021
, 47(24)
: 13
-20
.
DOI: 10.13995/j.cnki.11-1802/ts.027433
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