Food and Fermentation Industries >

2021 , Vol. 47 >Issue 24: 48 - 55

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027169

Identification and in vitro biological activity analysis of polyphenolic from Mohe blue honeysuckle

  • ZHU Guanbing ,
  • QIAO Jinli ,
  • HUYAN Wenjing ,
  • LIU Pei ,
  • ZHANG Yan ,
  • HUO Junwei
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  • 1(National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions, Harbin 150030, China)
    2(College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China)
    3(Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin 150030, China)

Received date: 2021-02-26

  Revised date: 2021-04-14

  Online published: 2022-01-21

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Abstract

In this study, the anthocyanins and non anthocyanin polyphenols of Mohe blue honeysuckle were isolated and identified by high performance liquid chromatography-electrospray tandem mass spectrometry (HPLC-ESI-MS2), and their antioxidant activity, lipase inhibition activity, α-amylase inhibition activity, and α-glucosidase inhibition activity were analyzed. The results showed that blue honeysuckle fruits in Mohe contained 8 kinds of anthocyanins, among which the proportion of cyanidin-3-O-glucoside was significantly high, and the content of 5-caffeoylquinic acid was the highest among 24 kinds of non-anthocyanins. The contents of the total phenolics and the total anthocyanins in crude extracts of blue honeysuckle fruits in Mohe reached (108.52±6.80) mg GAE/100g DW and (23.36±0.54) mg C3G/100g DW, which showed strong antioxidant activity (201.19±9.97) μmol TE/g DW, (5.16±0.16) μmol TE/g DW, (658.18±9.73) μmol TE/g DW, and (414.79±2.08) μmol TE/g DW against DPPH, FRAP, ABTS, and ORAC assays, respectively. The results also showed certain biological activity of the inhibition activity of α-amylase, α-glucosidase and lipase. The semi-inhibitory concentrations of α-amylase, α-glucosidase and lipase were 6.399 mg/mL, 11.73 mg/mL and 1.912 mg/mL, respectively. The results of this study provides scientific references and valuable insights for blue honeysuckle fruit in Mohe.

Key words： blue honeysuckle; polyphenols; antioxidant activity; starch hydrolase inhibition activity; lipase inhibition activity

Cite this article

ZHU Guanbing , QIAO Jinli , HUYAN Wenjing , LIU Pei , ZHANG Yan , HUO Junwei . Identification and in vitro biological activity analysis of polyphenolic from Mohe blue honeysuckle[J]. Food and Fermentation Industries, 2021 , 47(24) : 48 -55 . DOI: 10.13995/j.cnki.11-1802/ts.027169

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