Food and Fermentation Industries >

2021 , Vol. 47 >Issue 24: 77 - 82

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027163

Screening and identification of selenium-tolerant Lysinibacillus fusiformis D1 and scavenging activity on oxygen free radicals

  • DUAN Yuhua ,
  • YU Yonghe ,
  • YANG Rui ,
  • WANG Yidan ,
  • ZHOU Xin ,
  • WANG Zhangqian
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  • 1(College of Life Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China)
    2(School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430040, China)
    3(National R & D Center for Se-Rich Agricultural Products Processing, Wuhan Polytechnic University, Wuhan 430040, China)

Received date: 2021-03-04

  Revised date: 2021-04-09

  Online published: 2022-01-21

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Abstract

Selenium-contaminated soil may be harmful to the ecological environment and human health. However, there are few studies on the use of microorganisms as a remedy in China. A high selenium tolerance strain was isolated from the soil of the Jianghan Plain. The strain was identified, its growth kinetics were analyzed, its nano-selenium was characterized, and its free radical scavenging activity was evaluated. The results showed that strain D1 was Lysinibacillus fusiformis and the highest tolerance to sodium selenite was 35 mg/mL. The particle size of nano-selenium was (306.20 ±1.64) nm. The secondary metabolites of Lysinibacillus fusiformis D1 had certain DPPH radical scavenging ability (66.38±0.67)% and strong ABTS radical scavenging ability (99.94±0.08)%. The nano-selenium produced by this strain had certain hydroxyl radical scavenging ability, which was (59.81±5.09)%. This strain can provide a reference for the bioremediation of selenium-rich soils, and the scavenging free radicals in its secondary metabolites need to be further studied.

Key words： Lysinibacillus fusiformis; Se-tolerance; SeNPs; biological recovery; free radical

Cite this article

DUAN Yuhua , YU Yonghe , YANG Rui , WANG Yidan , ZHOU Xin , WANG Zhangqian . Screening and identification of selenium-tolerant Lysinibacillus fusiformis D1 and scavenging activity on oxygen free radicals[J]. Food and Fermentation Industries, 2021 , 47(24) : 77 -82 . DOI: 10.13995/j.cnki.11-1802/ts.027163

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