Food and Fermentation Industries >

2021 , Vol. 47 >Issue 24: 124 - 131

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027440

Screening of an extracellular polysaccharides producing Bacillus thuringiensis strain and its fermentation optimization

  • YANG Jing ,
  • GAO Zexin ,
  • ZHU Li ,
  • ZHAN Xiaobei
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  • 1(Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China)
    2(Wuxi Galaxy Biotech Co.Ltd., Wuxi 214125, China)

Received date: 2020-03-24

  Revised date: 2020-04-01

  Online published: 2022-01-21

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Abstract

Microbial exopolysaccharides (EPS) has potential application value due to its short production cycle and other advantages. In this paper, a strain with high yield of EPS was screened from natto and identified as Bacillus thuringiensi. To improve its EPS production, the fermentation medium and fermentation conditions of strain were optimized by single-factor experiments. The Plackett-Burman (PB) design was used to evaluate the importance of nine factors, and three important factors were selected. Box-Benhnken experimental design was used to get the final optimal culture: 22.6 g/L glucose, 9.3 g/L soybean meal, 2.0 g/L corn syrup, 0.5 g/L K2HPO4, 1.5 g/L NaH2PO4, 1.5 g/L MgSO4, 0.05 g/L MnCl2, pH 7.5, loading volume 70 mL, 34 ℃, inoculum size of 6%. Compared with the initial fermentation process, the EPS production increased by 133.33%. Using 7 L fermenter to amplify and verify, the yield of EPS increased to 6.56 g/L. Compared with the shake flask level, the EPS production increased by 123.1% and the EPS had nattokinase activity. It is expected to become a potential source of synthetic drugs.

Key words： exopolysaccharides; Bacillus thuringiensi; fermentation optimization; nattokinase activity

Cite this article

YANG Jing , GAO Zexin , ZHU Li , ZHAN Xiaobei . Screening of an extracellular polysaccharides producing Bacillus thuringiensis strain and its fermentation optimization[J]. Food and Fermentation Industries, 2021 , 47(24) : 124 -131 . DOI: 10.13995/j.cnki.11-1802/ts.027440

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