Effect of extraction method on the physicochemical properties and antioxidant activity of polysaccharides from Gymnadenia conopsea R. Br.

  • LI Fengwei ,
  • PENG Mengli ,
  • ZHU Yuanping ,
  • GAO Wenbin ,
  • YU Xiaohong
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  • 1(School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, China)
    2(College of Life Science, Cangzhou Normal University, Cangzhou 061001, China)

Received date: 2021-02-25

  Revised date: 2021-04-06

  Online published: 2022-01-21

Abstract

The effect of five extraction methods (hot water, enzyme assisted, ultrasonic assisted, ultrasonic-enzyme assisted and microwave assisted) on the yields, properties and antioxidant activities of Gymnadenia conopsea polysaccharides (GCP) were evaluated. The results showed that the yield of GCP was in the following order: enzyme assisted method (25.6%)>ultrasonic-enzyme assisted method (23.6%)>ultrasonic assisted method (19.25%)>hot water method (12.63%)>microwave assisted method (7.19%). The monosaccharides, composed mainly of mannose and glucose in five GCP samples was the same. GCP extracted by all five methods had similar typical Fourier transform infrared spectroscopy. These GCP possessed different antioxidant activities in terms of measurement of hydroxyl radicals scavenging capacity. The ones extracted by enzyme assisted and ultrasonic assisted extraction showed better antioxidant activity than others. These data showed that enzyme and ultrasonic assisted methods could be used for extracting functional polysaccharides from Gymnadenia conopsea R. Br..

Cite this article

LI Fengwei , PENG Mengli , ZHU Yuanping , GAO Wenbin , YU Xiaohong . Effect of extraction method on the physicochemical properties and antioxidant activity of polysaccharides from Gymnadenia conopsea R. Br.[J]. Food and Fermentation Industries, 2021 , 47(24) : 150 -155 . DOI: 10.13995/j.cnki.11-1802/ts.027159

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