In order to investigate the inhibition of cyclooxygenase-2 (COX-2) and antioxidant activities of Ganoderma lucidum, G. lucidum-Polygonatum odoratum, G. lucidum-Inonotus obliquus, G. lucidum-Panax ginseng and G. lucidum-Panax notoginseng fermentation broth, the contents of total polysaccharides, total saponins and total polypeptides of the fermentation liquid lyophilized powder were determined by spectrophotometry combined with colorimetric method. The optimal inhibition of COX-2 and antioxidant activities of G. lucidum fermentation broth were screened out. Meanwhile, the scavenging ability of DPPH free radical and ABTS cation free radical and inhibition ability of COX-2 were determined to evaluate the antioxidant and COX-2 inhibition activities in vitro. The results showed that compared with G. lucidum fermentation liquid lyophilized powder, G. lucidum-P. odoratum fermentation liquid lyophilized powder had strongest DPPH free radical and ABTS cation free radical scavenging ability and COX-2 inhibition ability, and the contents of total saponins and total peptides were 1.26 and 1.27 times higher than those of G. lucidum fermentation liquid lyophilized powder, respectively (P<0.05). Therefore, this study concluded that G. lucidum-P. odoratum fermentation broth showed the strongest COX-2 inhibition and antioxidant activity, which was related to the significant increase of total saponins and total polypeptides. G. lucidum fermentation by adding P. odoratum is the best combination of inhibiting COX-2 and antioxidant, which provides an idea for the industrial production of bidirectional fermentation of G. lucidum, it also laid a foundation for further study on the relationship between the content of each component of fermentation broth and its activity.
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